Skip to main content
SpringerLink
Log in

Protective effects of Radix Astragali injection on multiple organs of rats with obstructive jaundice

  • Original Article
  • Published:
Chinese Journal of Integrative Medicine Aims and scope Submit manuscript

Abstract

Objective

To investigate the protective effects and mechanisms of Radix Astragali Injection on multiple organs of rats with obstructive jaundice (OJ).

Methods

A total of 180 rats were randomly divided into the sham-operated, model control and treated groups (60 in each group). On 7, 14, 21 and 28 days after operation, the serum contents of alanine aminotransferase (ALT), aspartate aminotransferase (AST), r-glutamyl transpeptidase (r-GT), total bilirubin (TBil), direct bilirubin (DBil), blood urine nitrogen (BUN), and creatinine (CREA) were determined. And the pathological changes of livers, kidneys and lungs, and protein expressions of toll-like receptor-4 (TLR-4) of livers, intercellular adhesion molecule-1 (ICAM-1) of lungs, Bax and nuclear factor-kappa B (NF-κB), as well as apoptotic indexes of multiple organs were observed, respectively.

Results

The pathological severity scores of multiple organs (including livers on 7, 14, 21 and 28 days, kidneys on 14 and 28 days, and lungs on 14 days), serum contents of ALT (14 and 21 days), AST (14 days), TBil (7, 14, 21 and 28 days), DBil (14 and 21 days), BUN (28 days), protein expressions of TLR-4 (in livers, 28 days), Bax (in livers and kidneys, 21 days), and apoptotic indexes in livers (7 and 21 days) in the treated group were significantly lower than those in the model control group (P<0.05 or P<0.01).

Conclusion

Radix Astragali Injection exerts protective effects on multiple organs of OJ rats by improving the pathological changes of lung, liver and kidney, decreasing the serum index of hepatic and renal function as well as inhibiting the protein expression of TLR-4 and Bax in the livers and Bax in the kidneys.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Calikülekci M, Pata C, Apa DD, Dirlik M, Tamer L, Yaylak F, et al. The effect of N-acetylcysteine (NAC) on liver and renal tissue inducible nitric oxide synthase (iNOS) and tissue lipid peroxidation in obstructive jaundice stimulated by lipopolysaccharide (LPS). Pharmacol Res 2004;49:227–238.

    Article  Google Scholar 

  2. Penkov N. Pathogenetic mechanisms in biliary obstruction. Khirurgiia (Sofiia) 2003;59:39–45.

    CAS  Google Scholar 

  3. Kennedy JA, Lewis H, Clements WD, Kirk SJ, Campbell G, Halliday MI, et al. Kupffer cell blockade, tumour necrosis factor secretion and survival following endotoxin challenge in experimental biliary obstruction. Br J Surg 1999;86:1410–1414.

    Article  CAS  PubMed  Google Scholar 

  4. Scopa CD, Koureleas S, Tsamandas AC, Spiliopoulou I, Alexandrides T, Filos KS, et al. Beneficial effects of growth hormone and insulin-like growth factor on intestinal bacterial translocation, endotoxemia, and apoptosis in experimentally jaundiced rats. J Am Coll Surg 2000;190:423–431.

    Article  CAS  PubMed  Google Scholar 

  5. Hong JY, F Sato E, Hiramoto K, Nishikawa M, Inoue M. Mechanism of liver injury during obstructive jaundice: role of nitric oxide, splenic cytokines, and intestinal flora. J Clin Biochem Nutr 2007;40:184–193.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Peng B, Qiao AY, Luo YL, Li ZY, He S, Wu YT. The mechanism and significance of cellular signal transduction in kidney glomerular mesangial cells of rat with obstructive jaundice. J Sichuan Univ (Med Sci Ed) 2007;38:116–118.

    CAS  Google Scholar 

  7. Lalisang TJ, Sjamsuhidajat R, Siregar NC, Taher A. Profile of hepatocyte apoptosis and bile lakes before and after bile duct decompression in severe obstructive jaundice patients. Hepatobiliary Pancreat Dis Int 2010;9:520–523.

    PubMed  Google Scholar 

  8. Dirlik M, Canbaz H, Dümez Apa D, Calikülekçi M, Yaylak F, Balli E, et al. The monitoring of progress in apoptosis of liver cells in bile duct-ligated rats. Turk J Gastroenterol 2009;20:247–256.

    Article  PubMed  Google Scholar 

  9. Chen J. Pharmacological action and clinical application of Radix Astragali Injection. J Tianjin Med Univ (Chin) 2005;11:153–157.

    CAS  Google Scholar 

  10. Lin JJ, Jin CN, Zheng ML, Ouyang XN, Zeng JX, Dai XH. Clinical study on treatment of primary hepatocellular carcinoma by Shenqi Mixture combined with microwave coagulation. Chin J Integr Med 2005;11:104–110.

    Article  PubMed  Google Scholar 

  11. Yang QY, Chen KJ, Lu S, Sun HR. Research progress on mechanism of action of Radix Astragalus in the treatment of heart failure. Chin J Integr Med 2012;18:235–240.

    Article  PubMed  Google Scholar 

  12. Lu S, Chen KJ, Yang QY, Sun HR. Progress in the research of Radix Astragali in treating chronic heart failure: effective ingredients, dose-effect relationship and adverse reaction. Chin J Integr Med 2011;17:473–477.

    Article  PubMed  Google Scholar 

  13. Dou H, Fu TJ, Zhang F, Liu ZR, Ding LS. Chemical constituents of Huangqi Injection. Nat Prod Res Devel (Chin) 2002;14:14–17.

    CAS  Google Scholar 

  14. Zhang RP, Zhang XP, Ruan YF, Ye SY, Zhao HC, Cheng QH, et al. Protective effect of Radix Astragali Injection on immune organs of rats with obstructive jaundice and its mechanism. World J Gastroenterol 2009;15:2862–2869.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Zhang XP, Weng K, Yu YP, Zhao HC, Cheng QH. Protective effect and mechanisms of Radix Astragali Injection on the intestinal mucosa of rats with obstructive jaundice. Mediators Inflamm 2010;2010:757191.

    Google Scholar 

  16. Zhang XP, Wu DJ, Liang JF, Cheng QH, Ye J, Jia PH, et al. Effects of Salvia miltiorrhizae on ICAM-1, TLR4, NFkappaB and Bax proteins expression in multiple organs of rats with severe acute pancreatitis or obstructive jaundice. Inflammation 2009;32:218–232.

    Article  CAS  Google Scholar 

  17. Sakrak O, Akpinar M, Bedirli A, Akyurek N, Aritas Y. Short and long-term effects of bacterial translocation due to obstructive jaundice on liver damage. Hepato-Gastroenterology 2003;50:1542–1546.

    PubMed  Google Scholar 

  18. Wait RB, Kahng KU. Renal failure complicating obstructive jaundice. Am J Surg 1989;157:256–263.

    Article  CAS  PubMed  Google Scholar 

  19. Gordon S. Pattern recognition receptors: doubling up for the innate immune response. Cell 2002;111:927–930.

    Article  CAS  PubMed  Google Scholar 

  20. Johnson GB, Brunn GJ, Platt JL. Cutting edge: an endogenous pathway to systemic inflammatory response syndrome (SIRS)-like reactions through toll-like receptor 4. J Immunol 2004;172:20–24.

    Article  CAS  PubMed  Google Scholar 

  21. Wang KN, Xu M. Relationship of toll-like receptor 4, nuclear factor kappa B and acute pancreatitis. World Chin J Digest (Chin) 2007;15:2684–2689.

    CAS  Google Scholar 

  22. Zhou MY, Lo SK, Bergenfeldt M, Tiruppathi C, Jaffe A, Xu N, et al. In vivo expression of neutrophil inhibitory factor via gene transfer prevents lipopolysaccharide-induced lung neutrophil infiltration and injury by a beta2 integrindependent mechanism. J Clin Invest 1998;101:2427–2437.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Miyaso H, Morimoto Y, Ozaki M, Haga S, Shinoura S, Choda Y, et al. Obstructive jaundice increases sensitivity to lipopolysaccharide via TLR4 upregulation: possible involvement in gut-derived hepatocyte growth factor-protection of hepatocytes. J Gastroenterol Hepatol 2005;20:1859–1866.

    Article  CAS  PubMed  Google Scholar 

  24. Thatte U, Dahanukar S. Apoptosis: clinical relevance and pharmacological manipulation. Drugs 1997;54:511–532.

    Article  CAS  PubMed  Google Scholar 

  25. Ortiz A, Justo P, Sanz A, Melero R, Caramelo C, Guerrero MF, et al. Tubular cell apoptosis and cidofovir-induced acute renal failure. Antivir Ther 2005;10:185–190.

    CAS  PubMed  Google Scholar 

  26. Wang JM, Wang H, Xu LN, Zou SQ. Hepatic injury in rats with obstructive jaundice: roles of the protein kinase C signal pathway and cytoprotection of fructose. Hepatobiliary Pancreat Dis Int 2005;4:577–581.

    CAS  PubMed  Google Scholar 

  27. Wolter KG, Hsu YT, Smith CT, Nechushtan A, Xi XG, Youle RJ. Movement of Bax from the cytosol to mitochondria during apoptosis. J Cell Biol 1997;139:1281–1292.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Maurer M, Tsai M, Metz M, Fish S, Korsmeyer SJ, Galli SJ. A role for Bax in the regulation of apoptosis in mouse mast cells. J Invest Dermatol 2000;114:1205–1206.

    Article  CAS  PubMed  Google Scholar 

  29. Hill DB, Barve S, Joshi-Barve S, Mc Clain C. Increased monocyte nuclear factor-kappaB activation and tumor necrosis factor production in alcoholic hepatitis. J Lab Clin Med 2000;135:387–395.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of General Surgery, Xinhua Hospital, Chongming Branch, School of Medicine, Shanghai Jiao Tong University, Shanghai, 202150, China

    Zhe-wei Fei  (费哲为)

  2. Department of Breast Surgery, Zhejiang Cancer Hospital, Hangzhou, 310022, China

    Xi-ping Zhang  (张喜平)

  3. First College of Clinical Medicine, Zhejiang Chinese Medical University, Hangzhou, 310053, China

    Jie Zhang  (张 杰), Xin-mei Huang  (黄鑫梅) & Di-jiong Wu  (吴迪炯)

  4. Department of Genetics, Development, and Cell Biology, Iowa State University, Ames, 50010, USA

    Hong-hao Bi  (毕洪浩)

Authors
  1. Zhe-wei Fei  (费哲为)
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xi-ping Zhang  (张喜平)
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jie Zhang  (张 杰)
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xin-mei Huang  (黄鑫梅)
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Di-jiong Wu  (吴迪炯)
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Hong-hao Bi  (毕洪浩)
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xi-ping Zhang  (张喜平).

Additional information

Supported by the Foundation Project for Technology of Hangzhou, China (No. 2005224)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Fei, Zw., Zhang, Xp., Zhang, J. et al. Protective effects of Radix Astragali injection on multiple organs of rats with obstructive jaundice. Chin. J. Integr. Med. 22, 674–684 (2016). https://doi.org/10.1007/s11655-015-2048-y

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11655-015-2048-y

Keywords

Search

Navigation