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Lipopolysaccharide Induced Upregulation of β-1,4-Galactosyltransferase-I in Schwann cell

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Abstract

β4 Galactosylation of glycoproteins is one of the most important post-translational modifications. Recent studies have demonstrated that aberrant galactosylation associates with some inflammation diseases. β-1,4-galactosyltransferase-I (β-1,4-GalT-I), which transfers galactose to the terminal N-acetylglucosamine of N- and O-linked glycans in a β-1,4- linkage, considered to be the major galactosyltransferse among the seven members of the subfamily responsible for β4 galactosylation. In the present study, we investigated the expression of β-1,4-GalT-I in Schwann cells under Lipopolysaccharide (LPS) treatment. RT-PCR revealed that the β-1,4-GalT-I mRNA was significant increased as early as 2 h after LPS stimulation. Immunofluorescence showed that β-1,4-GalT-I was located in Golgi apparatus and membrane of Schwann cells. With the 1 μg/ml LPS treatment, expression levels of β-1,4-GalT-I was much higher compared with control group. In addition, lectin blot indicated that the β4 galactosylation of glycoproteins such as integrin α5 was enhanced, which may due to the induced β-1,4-GalT-I expression. These results suggested that β-1,4-GalT-I may play an important role in adhesion and migration of Schwann cells during inflammation.

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Authors and Affiliations

  1. Department of Orthopaedics, Affiliated Jiangyin Renmin Hospital of Nantong University, Key Laboratory for Neuroregeneration of JiangSu Province, Nantong University, 19 Qiqiu Road, Nantong, 226001, Jiangsu, People’s Republic of China

    Huiguang Yang, Xiaohong Li & Aiguo Shen

  2. Department of Parasitology, Medical College, Nantong University, Nantong, People’s Republic of China

    Ling Hu, Jianping Chen, Jianchun Zhu, Tao Tao, Fupeng Zhang, Xingxin He & Chun Cheng

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  1. Huiguang Yang
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Correspondence to Aiguo Shen or Chun Cheng.

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Huiguang Yang and Ling Hu contributed equally to this work.

Grant sponsor: National Natural Science Foundation of China (no.30770488 and no. 30870320); Natural Science Foundation of Jiangsu province (no.BK2006547); Health Project of Jiangsu Province (H200632).

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Yang, H., Hu, L., Chen, J. et al. Lipopolysaccharide Induced Upregulation of β-1,4-Galactosyltransferase-I in Schwann cell. Inflammation 32, 279–286 (2009). https://doi.org/10.1007/s10753-009-9131-5

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