Skip to main content
SpringerLink
Log in

New endo-β-1,4-glucanases from the parabasalian symbionts, Pseudotrichonympha grassii and Holomastigotoides mirabile of Coptotermes termites

  • Published:
Cellular and Molecular Life Sciences CMLS Aims and scope Submit manuscript

Abstract.

An endo-β-1,4-glucanase (EG) was purified from the hindgut of an Australian mound-building termite, Coptotermes lacteus. The hindgut extract had a peak separate from those for extracts obtained from the salivary glands and the midgut based on sephacryl S-200 gel chromatography, and also demonstrated an origin different from the endogenous EGs of the termite itself. The recovery was further purified by SDS-PAGE, and its N-terminal amino acid sequence analyzed. This showed high homology to EGs from glycoside hydrolase family (GHF) 7. PCR-based cloning methods were applied to the hindgut contents of C. lacteus and individual protozoan symbionts from C. formosanus. cDNAs encoding putative EGs homologous to GHF7 members were then identified. The functionality of one of the putative proteins was confirmed by its expression in Escherichia coli.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Author information

Authors and Affiliations

  1. National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8634 (Japan), Fax +81 298 38 6108, e-mail: hinabe@affrc.go.jp, , , , , , JP

    H. Watanabe & H. Saito

  2. Division of Environmental Science and Technology, Graduate School of Agricultural Science, Kyoto University, Sakyo-ku, Kyoto 606-8502 (Japan), , , , , , JP

    K. Nakashima

  3. Department of Biochemistry, The University of Sydney, Sydney, New South Wales 2006 (Australia), , , , , , AU

    M. Slaytor

Authors
  1. H. Watanabe
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. Nakashima
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. Saito
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Slaytor
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Received 18 September 2002; accepted 20 September 2002

RID="*"

ID="*"Corresponding author.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Watanabe, H., Nakashima, K., Saito, H. et al. New endo-β-1,4-glucanases from the parabasalian symbionts, Pseudotrichonympha grassii and Holomastigotoides mirabile of Coptotermes termites. CMLS, Cell. Mol. Life Sci. 59, 1983–1992 (2002). https://doi.org/10.1007/PL00012520

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/PL00012520

Search

Navigation