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Arthritis Induced by Adjuvant in Spontaneously Hypertensive and Normotensive Rats: Endogenous Glucocorticoid Effects on Inflammatory Response

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Abstract

The present study investigated arthritis induced by complete Freund adjuvant (AIA) in spontaneously hypertensive and normotensive rats (respectively, SHR and NTR rats). The inflammatory reaction was studied for 28 days by evaluating paw edema and secondary lesions found 10 days after complete Freund adjuvant (CFA) administration. The body weight of the animals and macroscopic alterations of several organs, including spleen, thymus, adrenal glands, and lymph nodes, were also analyzed. The results showed that the AIA manifestations were decreased in SHRs compared with NTRs. Moreover, this altered inflammatory response was not modified by surgical adrenalectomy.

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References

  1. Trippodo, N. C., and E. D. Frohlich. 1981. Similarities of genetic (spontaneous) hypertension: man and rat. Circ Res. 8:309–19.

    Google Scholar 

  2. Aoki, K., H. Tankawa, T. Fujinami, A. Miyazaki, and Y. Hashimoto. 1963. Pathological studies on the endocrine organs of the spontaneously hypertensive rats. Jpn Heart J. 186:426–42.

    PubMed  CAS  Google Scholar 

  3. Hashimoto, K., S. Makino, R. Hirasawa, T. Takao, M. Sugawara, K. Murakami, K. Ono, and Z. Ota. 1989. Abnormalities in the hypothalamo-pituitary-adrenal axis in spontaneously hypertensive rats during development of hypertension. Endocrinology. 125:1161–7.

    Article  PubMed  CAS  Google Scholar 

  4. Dzielak, D. J. 991. Immune mechanisms in experimental and essential hypertension. Am J Physiol. 260:459–67.

    Google Scholar 

  5. Schmid-Schönbein, G. W., D. Seiffge, F. A. DeLano, K. Shen, and B. W. Zweifach. 1991. Leukocyte counts and activation in spontaneously hypertensive and normotensive rats. Hypertension. 17:323–30.

    PubMed  Google Scholar 

  6. Sirén, A. L., E. Heldman, D. Doron, P. G. Lysko, T. L. Yue, Y. Liu, G. Feuerstein, and J. M. Hallenbeck. 1992. Release of proinflammatory and prothrombotic mediators in the brain and peripheral circulation in spontaneously hypertensive and normotensive Wistar-Kyoto rats. Stroke. 23:1643–51.

    PubMed  Google Scholar 

  7. McCarron, R. M., L. Wang, A. L. Siren, M. Spatz, and J. M. Hallenbeck. 1994. Adhesion molecules on normotensive and hypertensive rat brain endothelial cells. Proc Soc Exp Biol Med. 205:257–62.

    PubMed  CAS  Google Scholar 

  8. Kwasniewski, F. H., W. Tavares de Lima, Y. S. Bakhle, and S. Jancar. 1998. Impairment in connective tissue mast cells degranulation in spontaneously hypertensive rats: stimulus dependent resistance. Br J Pharmacol. 124:772–8. doi:10.1038/sj.bjp.0701875.

    Article  PubMed  CAS  Google Scholar 

  9. Ferreira, A. A., F. H. Kwasniewski, T. C. Delani, M. G. Torres, M. A. Silva, S. M. Caparroz-Assef, R. K. Cuman, and C. A. Bersani-Amado. 2007. Acute immune and non-immune inflammatory response in spontaneously hypertensive rats and normotensive rats: role of endogenous nitric oxide. Inflammation. 30:198–204. doi:10.1007/s10753-007-9037-z.

    Article  PubMed  CAS  Google Scholar 

  10. Takeichi, N., K. Suzuki, T. Okayasu, and H. Kobayashi. 1980. Immunological depression in spontaneously hypertensive rats. Clin Exp Immunol. 40:120–6.

    PubMed  CAS  Google Scholar 

  11. Pearson, C. M. 1956. Development of arthritis, periarthritis and periostitis in rats given adjuvants. Proc Soc Exp Biol Med. 91:95–101.

    PubMed  CAS  Google Scholar 

  12. Owen, R. T. 1980. Adjuvant induced polyarthritis: an overview. Methods Find Exp Clin Pharmacol. 2:199–204.

    PubMed  CAS  Google Scholar 

  13. Caparroz-Assef, S. M., C. A. Bersani-Amado, A. M. Kelmer-Bracht, A. Bracht, and E. L. Ishii-Iwamoto. 2007. The metabolic changes caused by dexamethasone in the adjuvant-induced arthritic rat. Mol Cell Biochem. 302:87–98. doi:10.1007/s11010-007-9430-9.

    Article  PubMed  CAS  Google Scholar 

  14. Pearson, C. M., and F. D. Wood. 1963. Studies of arthritis and other lesions induced in rats by the injection of mycobacterial adjuvant: VII. Pathologic details of the arthritis and spondylitis. Am J Pathol. 42:73–95.

    PubMed  CAS  Google Scholar 

  15. Rosenthale, M. E. 1970. A comparative study of the Lewis and Sprague Dawley rat in adjuvant arthritis. Arch Int Pharmacodyn Ther. 188:14–22.

    PubMed  CAS  Google Scholar 

  16. Kwasniewski, F. H., W. Tavares de Lima, Y. S. Bakhle, and S. Jancar. 2003. Endogenous nitric oxide does not modulate mesenteric mast cell degranulation in rats. Biochem Pharmacol. 65:2073–80. doi:10.1016/S0006-2952(03)00191-6.

    Article  PubMed  CAS  Google Scholar 

  17. Kwasniewski, F. H., R. G. Landgraf, Y. S. Bakhle, and S. Jancar. 2004. Bronchoconstriction and endogenous nitric oxide in isolated lungs of spontaneously hypertensive rats. Eur J Pharmacol. 488:181–9. doi:10.1016/j.ejphar.2004.02.005.

    Article  PubMed  CAS  Google Scholar 

  18. Muir, V. Y., and D. C. Dumonde. 1982. Different strains of rats develop different clinical forms of adjuvant disease. Ann Rheum Dis. 41:538–43. doi:10.1136/ard.41.5.538.

    Article  PubMed  CAS  Google Scholar 

  19. Sternberg, E. M., J. M. Hill, G. P. Chrousos, T. Kamilaris, S. J. Listwak, P. W. Gold, and R. L. Wilder. 1989. Inflammatory mediator-induced hypothalamic-pituitary-adrenal axis activation is defective in streptococcal cell wall arthritis-susceptible Lewis rats. Proc Natl Acad Sci USA. 86:2374–8. doi:10.1073/pnas.86.7.2374.

    Article  PubMed  CAS  Google Scholar 

  20. Kobayashi, N., and G. W. Schmid-Schönbein. 2006. Intrinsic disturbance of cellular redox balance enhances blood lymphocyte apoptosis in the spontaneously hypertensive rat. Free Radic Biol Med. 41:484–92. doi:10.1016/j.freeradbiomed.2006.04.016.

    Article  PubMed  CAS  Google Scholar 

  21. van Loveren, H., R. Meade, and P. W. Askenase. 1983. An early component of delayed-type hypersensitivity mediated by T cells and mast cells. J Exp Med. 157:1604–17. doi:10.1084/jem.157.5.1604.

    Article  PubMed  Google Scholar 

  22. Biedermann, T., M. Kneilling, R. Mailhammer, K. Maier, C. A. Sander, G. Kollias, S. L. Kunkel, L. Hültner, and M. Röcken. 2000. Mast cells control neutrophil recruitment during T cell-mediated delayed-type hypersensitivity reactions through tumor necrosis factor and macrophage inflammatory protein 2. J Exp Med. 192:1441–52. doi:10.1084/jem.192.10.1441.

    Article  PubMed  CAS  Google Scholar 

  23. Schmid-Schönbein, G. W., D. Seiffge, F. A. Delano, K. Shen, and B. W. Zweifach. 1991. Leukocyte counts and activation in spontaneously hypertensive and normotensive rats. Hypertension. 17:323–30.

    PubMed  Google Scholar 

  24. Guglielmotti, A., D’Onofrio, E., Coletta, I., Aquilini, L., Milanese, C., Pinza, M. 2002. Amelioration of rat adjuvant arthritis by therapeutic treatment with bindarit, an inhibitor of MCP-1 and TNF-α production. Inflamm Res. 51:252–8. doi:10.1007/PL00000301.

    Article  PubMed  CAS  Google Scholar 

  25. Eklund, K. K. 2007. Mast cells in the pathogenesis of rheumatic diseases and as potential targets for anti-rheumatic therapy. Immunol Rev. 217:38–52. doi:10.1111/j.1600-065X.2007.00504.x.

    Article  PubMed  CAS  Google Scholar 

  26. Gryfe, A., P. M. Sanders, and D. L. Gardner. 1971. The mast cell in early rat adjuvant arthritis. Ann Rheum Dis. 30:24–30. doi:10.1136/ard.30.1.24.

    Article  PubMed  CAS  Google Scholar 

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Acknowledgements

We gratefully acknowledge financial support received from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). We also thank Mr. Jailson Araújo Dantas and Mrs. Célia Regina Miranda for technical assistance.

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Authors and Affiliations

  1. Laboratory of Inflammation, Department of Pharmacy and Pharmacology, University of Maringá, Maringá, Paraná, Brazil

    Micheli G. Torres, Luís G. Scaliante, Silvana M. Caparroz-Assef, Roberto K.N. Cuman & Ciomar A. Bersani-Amado

  2. Núcleo Integrado de Biotecnologia, University of Mogi das Cruzes, Mogi das Cruzes, São Paulo, Brazil

    Fabio H. Kwasniewski

  3. Department of Biochemistry, University of Maringá, Maringá, Paraná, Brazil

    Emy L. Ishii-Iwamoto

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  1. Micheli G. Torres
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  2. Fabio H. Kwasniewski
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  3. Luís G. Scaliante
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Correspondence to Ciomar A. Bersani-Amado.

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Torres, M.G., Kwasniewski, F.H., Scaliante, L.G. et al. Arthritis Induced by Adjuvant in Spontaneously Hypertensive and Normotensive Rats: Endogenous Glucocorticoid Effects on Inflammatory Response. Inflammation 32, 20–26 (2009). https://doi.org/10.1007/s10753-008-9098-7

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