Abstract
Understanding how local populations respond to specific changes in the environment can help us better predict how populations respond to such change. With this topic in mind, we followed up on a previous study by exploring the capabilities of a Geophagini cichlid, known for its unique feeding strategy, to mount a plastic response. We exposed Satanoperca daemon, a winnowing cichlid, to three different substrate types, two of which encouraged winnowing behaviors and a third that prevented winnowing entirely. Using geometric morphometrics, we quantified aspects of craniofacial anatomy to test for morphological differences between the treatments and to test for the integration of different traits across the head. We found significant differences across our experimental populations in both shape and disparity. We report evidence in support of wide-spread integration across craniofacial traits. A notable exception to this pattern was the epibranchial lobe, a structure unique to the Geophagini, which exhibited more modular variation. Since anthropogenic alterations such as the damming of rivers can impact substrate type, these data offer insights into how Geophagini cichlids may respond to environmental change. In addition, this work further illuminates the functional morphology of winnowing foraging behaviors.
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Introduction
The Cichlidae contain an enormous diversity of fishes that have exploited different niches, environments, and behaviors. Cichlids are distributed across Africa, South America, India, and Madagascar and have been the focus of study for decades, becoming a modern model for understanding complex evolutionary and ecological processes and for digging into evo-devo concepts (Muschick et al., 2012; Arbour & López-Fernández, 2013; Feilich ,2016; Mcgee et al., 2016; Powder & Albertson, 2016; Navon et al., 2020). Much of the work regarding adaptive radiations has focused on African cichlids (Turner, 2007), undoubtedly due to the nature of the rift lake system. However, it has been shown that South American cichlids have also gone through rapid radiation events, most notably within the Geophagini clade (López-Fernández et al., 2013; Burress et al., 2018, 2022).
Plasticity in cichlids has been extensively noted and includes numerous aspects of craniofacial morphology (Chapman et al., 2000; Muschick et al., 2011). Because of this, plasticity has become incorporated as a key concept of how the diversity of cichlids has arisen, particularly through the feeding apparatus representing a flexible stem (West-Eberhard, 1989, 2003). This extends to the South American cichlids where plasticity has been demonstrated in the Geophagini cichlid genus Geophagus (Wimberger, 1991, 1992, 1993). Here, through a series of experiments, Wimberger was able to induce a plastic response in two species (Geophagus steindachneri and G. brasiliensis) by changing the prey available to experimental populations, thereby altering the predatory approach of the different populations. The Geophagini are so named (i.e., “earth eating”) due to their unique behavior of winnowing, a process of taking in mouthfuls of sediment and sifting out detritus and invertebrates. Distinctive to this group is a highly modified epibranchial lobe on the first gill arch that is speculated to have evolved to facilitate winnowing (López-Fernández et al., 2012; Weller et al., 2022), although its exact kinematic role remains elusive.
The epibranchial lobe possesses some degree of variation tied to diet and ecomorphology (López-Fernández et al., 2012). However, it is unknown how specific aspects of the morphology of the epibranchial lobe associate with different substrates and/or prey items. Further, the degree to which the epibranchial lobe is plastic remains largely unknown, as winnowing was not explored in early experiments by Wimberger (1991, 1992, 1993). Previously published work detailed significant morphological change in the winnowing cichlid species Satanoperca jurupari (Heckel 1840) and Geophagus neambi (Lucinda, Lucena and Assis 2010) following the damming of a major river system in Brazil, suggesting that radical environmental alterations had a substantial effect on the (external) cranial morphology of this species (Gilbert et al., 2020).
Because the alteration of a riverine system to a lacustrine system can substantially alter sediment type (Trautman, 1939; Allan & Flecker ,1993; Lau et al., 2006; Helfman, 2007), we sought to understand if different substrates (i.e., rock, sand, or substrate-free) could induce a plastic morphological change in a winnowing cichlid. Specifically, we exposed the winnowing species, Satanoperca daemon (Heckel 1840), to alternate substrate types and quantified morphological variation across a suite of foraging-related traits. We predicted that the population exposed to larger sediments would develop deeper heads, larger muscles, and altered oral cavities, including the epibranchial lobes, to sift through the heavy, coarse substrate. Next, we tested for correlations between various craniofacial traits to identify sets of traits that exhibited an integrated plastic response. We predicted that functionally related traits, such as the oral jaws and adductor muscles as well as the oral cavity and epibranchial lobes, would covary. Together, these results will aid in discerning how winnowing species may adapt their feeding architecture in response to rapid environmental change, such as damming. In addition, levels of phenotypic integration across various aspects of foraging anatomy, may provide further insights into the kinematics of winnowing behavior including roles for the novel epibranchial lobe.
Methods
Experimental design
We obtained wild caught Satanoperca daemon juveniles from local importers who specialized in acquiring fishes from the Central and South Americas. Upon arrival, we parsed fish into one of three different 40 gallon aquaria. To evaluate the effect of substrate composition on winnowing morphology, we modified the substrate in each tank. Tank one (n = 15) contained no substrate and fish were fed twice daily with bloodworms provided on the surface of the water. The lack of substrate in this tank prevented winnowing from occurring at all, even when food was absent. Tank two (n = 17) possessed sand substrate, fish were fed twice daily with bloodworms that had been covered with sand in a 2″ deep Pyrex dish. Tank three (n = 11) possessed a substrate comprised of aquarium gravel and fish were fed twice daily with bloodworms that had been covered with identical gravel in a 2″ deep Pyrex dish. Since Satanoperca had been previously observed to winnow casually, even outside of feeding hours, tanks two and three had substrate in their tanks continuously to reinforce winnowing behaviors even while food was absent. However, tank one never possessed substrate of any kind to prevent the mechanical process of winnowing entirely. This experiment was kept active for 27 weeks and were fixed following MS-222 (Tricaine methane sulfonate) overdose coupled with ice shock. The body size (standard length) ranged from 5 cm to about 9 cm.
Specimen preparation and data collection
Fixed fish were stained using an alizarin solution in 75% EtOH to visualiz osteological elements but preserve muscle integrity and morphology for data acquisition. We then carefully dissected all specimens to reveal the adductor mandibularis Pars malaris (A1) and Par rictalis (A2), and subsequent layers of muscles and bone were systematically removed, and collected, to ensure imagery of the dentary, angulo-articular, lachrymal, first epibranchial, and buccal cavity (including parasphenoid and hyoglossal—pharyngeal jaws; Fig. 1; Davoto & Vari, 2013).
We collected two-dimensional geometric morphometric data for the structures using Stereomorph v1.6.7 (Olsen & Westneat, 2015) in R (R Core Team, 2018). These two-dimensional morphometrics allowed us to encapsulate relevant shape data in a planar fashion. This collection method enabled us to analyze the craniofacial region in descending levels and keep the planes of analysis consistent to create an overarching view of this region of interest. In total, we collected morphometric data across 7 unique image sets. For adductor mandibularis and the orbit, five fixed and 24 semi-landmarks, 12 of which formed a continuous curve. Lachrymal, 10 fixed and six semi-landmarks. Anguloarticular, four fixed and nine semi-landmarks. Quadrate, four fixed landmarks and eight semi-landmarks. Dentary, six fixed and 12 semi-landmarks. For the first gill arch, including the epibranchial, four fixed and 10 semi-landmarks. For the oral cavity, six fixed and 10 semi-landmarks. See Fig. 2.
Geometric morphometric analyses
Unless otherwise stated, all analyses here were conducted using geomorph v3.3.6 (Adams et al., 2014, 2018). All geometric morphometric data were initially put through a generalized Procrustes analysis (GPA; Goodall, 1991) utilizing bending energy in geomorph (gpagen). Using a Procrustes ANOVA, we tested for differences in mean shape between treatment groups within each GM dataset [e.g., (shape ~ centroid size + treatment)] using an RRPP procedure (Collyer & Adams .2018) and 9999 + 1 iterations. We then regressed PC1 values against centroid size to visualize differences in allometry across the seven unique image sets. Branching from the initial Procrustes ANOVA, we conducted post-hoc pairwise comparisons using the function advanced.procD.lm in geomorph. Tests of morphological disparity were conducted using the function morphol.disparity in geomorph. Lastly, using the geomorph function two.b.pls (Rohlf & Corti, 2000; Adams & Collyer, 2016, 2019), we tested for correlations between each of the different anatomical elements included in this study [e.g., (two.b.pls (shape 1, shape 2, iter = 9999)]. All post-hoc tests were adjusted by calculating a Bonferroni p-value using the function p.adjust in R (R Core Team ,2018).
Results
Foraging environment induces mean shape differences for numerous traits
Our feeding treatments had a significant effect on 5/7 anatomical components investigated (Table 1), the two exceptions being the anguloarticular (p = 0.138) and quadrate (p = 0.986). When compared to the z-score of centroid size, the effect of substrate had the greatest influence on the oral cavity (z = 3.245 compared to CS, z = 1.957), whereas it had the lowest effect on the quadrate (z = − 2.089 compared to CS, z = 2.946). Centroid size was found to be significant in all treatments, but the interaction of centroid size and treatment was significant only in the orbit plus adductor mandibulae (Table 1). A plot of allometry (Fig. 3) shows a combination of differences, suggesting that the allometries of various aspects of craniofacial anatomy differ in shape depending on the treatment. For example, allometries of the three different treatments appear parallel to one another in both the lachrymal and dentary (relatively so for the quadrate), but the rock treated population diverges from this in the articular. Conversely, the pelagic treated fish diverge from the parallel allometries in the orbit plus adductor mandibulae and none of treatments appear to have parallel allometries for the oral cavity and first gill arch plus epibranchials.
Post-hoc pairwise comparisons (Table 2) showed pelagic treated fish differed significantly from both sand and rock treated populations (p = 0.013, 0.001 respectively; rock only following adjustment, p = 0.0077) in orbit plus adductor mandibulae shapes. Pelagic treated fish also differed in both dentary and oral cavity shapes when compared to rock treated fish (p = 0.018, 0.002; oral cavity only following adjustment, p = 0.0112). Fish from the rock and sand treatment differed in oral cavity and gill arch shapes (p = 0.0012, 0.0426; oral cavity only following adjustment, p = 0.0084).
Deformation grids of substrate groups showed that our sand winnowing population often expressed an intermediate phenotype compared to pelagic feeding and rock winnowing fish (Fig. 4). Overall, the rock winnowing population was characterized by larger adductor muscles relative to the orbit, a gracile coronoid process, truncated but stout epibranchial lobes, and a steeply curved parasphenoid and ventrally shifted hyoid series creating a relatively large oral cavity. The pelagic feeding population is characterized by reduced adductor muscles relative to the orbit, a somewhat robust coronoid process, elongated epibranchial lobes, and a substantially smaller oral cavity.
Foraging environment induces few differences in morphological disparity between populations
Across all seven anatomical components, only two pairwise comparisons (both involving the lachrymal) showed significantly different levels of disparity (Table 3). The sand winnowing population had significantly more variance (σ2 = 0.00467) in lachrymal shape than both the pelagic feeding population (σ2 = 0.00261, p = 0.0161) and rock winnowing population (σ2 = 0.00285, p = 0.0418). However, following a Bonferroni adjustment, no comparisons were found to be statistically significant with α = 0.05.
Orbit plus adductor shape is correlated with numerous other traits whereas the epibranchial lobe is largely modular
We implemented numerous partial least squares (PLS) tests to assess the degrees of integration in plasticity between each aspect of anatomy (Table 4, Fig. 5). Because quadrate shape was statistically the same across all three test populations, this component was not included in the PLS assay. Of the 15 PLS tests, 7 revealed a significant correlation. The orbit/adductor mandibulae had the greatest number of significant shape correlations (4/5 tests being significant before and after Bonferroni adjustment). Of these, the strongest correlation, and the strongest correlation of all trait comparisons, was with the lachrymal (R2 = 0.6727, p < 0.0001). The trait that had the fewest significant shape correlations was the first gill arch including the epibranchial lobe, which only correlated with oral cavity shape (R2 = 0.2972, p = 0.022; no significant correlation post Bonferroni adjustment).
Discussion
Substrate size induces plasticity in winnowing cichlid anatomy
The results of morphological analyses on multiple elements of craniofacial anatomy suggest that different substrate composition alone is sufficient to induce plastic responses in various aspects of feeding morphology in the winnowing cichlid Satanoperca daemon. Winnowing, a behavior that utilizes suction feeding to bring large amounts of sediment into the oral cavity, parsing of sediments and food items, and subsequent ejection of sediment (Weller et al., 2017) is an underexplored feeding strategy. While common among the Geophagini cichlids, it is rare across the Cichlidae family. Comparisons of mean morphological shape across three different foraging environments show that winnowing in rocky sediments induces the most drastic changes. Of six significant pairwise differences in mean shape, five involved the rock treatment. Only a single pairwise comparison, orbit plus muscles, was significant for sand vs pelagic treatments. Thus, if we consider plasticity to be a proxy for kinematic demands, rock-winnowing appears to be the most challenging treatment, whereas sand-winnowing and pelagic foraging seem to impose similar demands on the feeding apparatus.
Lab-based results are similar to patterns of morphologic change following a major anthropogenic disturbance
The construction of the Tucurui Hydroelectric Dam in 1984 introduced numerous changes in the system (Araújo-Lima et al., 1995; Eletrobrás/Dnaee ,1997; Fearnside, 2001), altering it from a riverine to lacustrine that included shifts in sediment composition (Trautman, 1939; Allan & Flecker, 1993; Lau et al., 2006; Helfman, 2007. Our previous work in this system documented statistically significant differences in the gross morphology, pre- and post-dam construction, within two Geophagini cichlids, Geophagus neambi and Satanoperca jurupari (Gilbert et al., 2020). We speculated that changes in sediment, from rocky to silty, had influenced the morphology of the Geophagini, possibly through phenotypic plasticity. It is also possible that a deeper reservoir habitat would provide greater opportunity for pelagic modes of feeding (e.g., ram/suction). Here, we show that substrate type alone (including substrate-free habitat) is indeed sufficient to induce morphological change in a closely related winnowing cichlid. It is therefore possible that the changes in morphology that we reported in 2020 were, at least in part, due to alterations of the substrate. However, we did not previously report on specific elements of craniofacial anatomy and next steps should work to directly compare anatomical elements of specimens from the two studies. The trend of effectively shifting mean shape values in phenotypic space but keeping interpopulation variation stable was also consistent with our previous paper (Gilbert et al., 2020), where we reported differences in whole body shape means, but not disparity, among cichlid species.
Functional implications of plastic changes
Morphological plasticity in fishes, especially cichlids, has been heavily documented (Chapman et al., 2000; Alexander & Adams, 2004; Lema & Nevitt, 2006; Muschick et al., 2011) and others have reported plasticity in related taxa, such as Geophagus brasiliensis and steindachneri (Wimberger, 1991, 1992). To our knowledge, this is the first report of sediment-induced morphological plasticity in a winnowing cichlid. Understanding the specific mechanical forces that have precipitated these changes will be an important topic for future study. However, the patterns of variation (and covariation) observed here lead to a number of predictions concerning the functional morphology of winnowing.
The differences observed in morphology are likely related to the effort required to suction and process substrates of radically different size. Satanoperca that winnowed rock, compared to the non-winnowers (pelagic), had substantially larger adductor muscles, larger buccal cavities, and parasphenoids with a higher arch. These traits could be associated with the cycling motions of the jaw when winnowing is occurring and room to generate increased water flow and turbulence to parse food items out of substrate (Van Wassenbergh et al., 2015; Brooks et al., 2018; Weller et al., 2022). Further, fish from both the sand and pelagic treatments had relatively wider coronoid processes of the dentary, compared to fish that winnowed rock. Since this process is where the ligament that connects the upper and lower jaws inserts, we speculate that a wider insertion point is due to forces being propagated to the coronoid during jaw protrusion/suction feeding, consistent with suction being an important first step in winnowing fine (but not coarse) substrate (Weller et al., 2017). Winnowers had shorter epibranchial lobes, with more room between the lobe itself and the rest of the gill arch, compared to non-winnowing fish. This is intuitive, given that this is the area where food items are being parsed from substrate. Anecdotal evidence in support of direct contact between substrate and the epibranchial lobe includes the observation that rock winnowing cichlids possessed gill rakers that were generally more mineralized than those from other treatments (gill raker number did not vary between treatments—data not shown). Finally, we found no correlation in morphology between the first gill arch/epibranchial lobe with the other traits, consistent with a modular plastic response relative to other aspects of the head (Cheverud, 1996; Cheverud et al., 1997; Klingenberg, 2008; Navon et al., 2020).
Modular vs. integrated plasticity and paths of least evolutionary resistance
This work demonstrates how plasticity can facilitate studies of morphological integration, concepts that are not normally considered together (but see Matesanz et al., 2021; Earley et al., 2012). For instance, since the study of co-variation (e.g., integration) relies on there being sufficient levels of variation (Gonzalez et al., 2011a,b; Hallgrímmson et al., 2002), and plasticity can produce novel sources of variation (West-Eberhard, 1989, 2003), it can be an effective tool in this realm. Moreover, since craniofacial/skeletal plasticity generally occurs during later life-history stages, its application can help to decouple the forces that may affect morphological integration, developmental versus functional, in particular. Of course, it is important to acknowledge that given the hierarchical nature of organismal development and growth, factors that influence morphological integration during early life-history will “carry-over” into later stages. Hallgrímmson has used a palimpsest metaphor to describe this (Hallgrímmson, 2009), whereby information is repeatedly written and erased from the same tablet but traces remain each time. Thus, anatomical units that participate in the same function are likely to become morphologically integrated, owing to a common load regime, but the strength of integration may also be influenced by a shared developmental history.
Regardless of the source, the subdivision of different biological systems into different modules is hypothesized to facilitate the evolvability of various traits (Wagner et al., 2007; Larouche et al., 2018; Zelditch & Goswami, 2021). Modular traits are often evolutionarily decoupled (less integrated), allowing the two or more traits to evolve independently to serve multiple, even disparate, functions (Cheverud, 1996). The independence of traits can be explained by the weakening of evolutionary constraints between them (Jacob, 1977; Wagner & Misof ,1993; Klingenberg, 2008; McGhee, 2007; Hallgrimsson et al., 2009; Sheftel et al., 2013). Indeed, it has been hypothesized that the evolution of novel traits is, in part, the result of an increasingly modular system developing (Prum, 2005a; Kuratani, 2009; Niwa et al., 2010; Gilbert et al., 2021). Thus, it is plausible that increasing modularity can lead to increased rates of evolution in lineages, thereby promoting speciation in diverse, complex environments (West-Eberhard, 1989, 2003; Foote, 1997).
Challenging fishes with various foraging environments provides an excellent opportunity to quantify magnitudes of both shape variation and covariation, thereby enabling assessments of phenotypic integration (Conith and Albertson ,2021; Navon et al., 2020). We document various levels of integration across craniofacial traits. On one end of the spectrum, orbit-adductor mandibulae shape is integrated with most other traits, suggesting that these traits are more likely to co-evolve along an evolutionary path of least resistance (Schluter, 1996; Foote, 1997; Conith & Albertson, 2021). Alternatively, the first gill arch including the epibranchial lobe is less integrated with the other aspects of anatomy measured, only covarying with the oral cavity. We hypothesize that the morphological decoupling of the first gill arch from other craniofacial elements and its plasticity have allowed, in part, for the Geophagini to take advantage of a novel niche environment. Given phenotypic plasticity can lead to the evolution of phenotypic novelties (Moczek, 2008), it is possible that this mechanism facilitated the evolution of the epibranchial lobe (a novelty specific to Geophagini cichlids), and that its decoupled, seemingly modular nature allowed it to be modified without influencing other aspects of the head. Further, we speculate that the retention of plasticity in the epibranchial lobe paved the way for multifunctionality to evolve in certain lineages, including the co-option of this functional complex for mouthbrooding, without altering other aspects of prey processing, a hypothesis recently posed by Weller et al., (2022).
Conclusion
All in all, we show that a simple, but ecologically relevant, shift in foraging habitat can lead to subtle but widespread shifts in anatomy. These lab-based experiments have the potential to inform patterns observed in nature, both in terms of how species may adapt (or not) to rapid, human-induced, environmental change, as well as the evolutionary origin and diversification of complex phenotypes. Further, we demonstrate that the quantification of phenotypic (co)variation following the introduction of foraging challenges is a useful paradigm for understanding the kinematics of feeding. Finally, we suggest that while winnowing represents a strange and somewhat enigmatic foraging strategy, it holds excellent potential as a study system to draw connections between ecology, development, anatomy, function and evolution.
Data availability
Data publicly available on GitHub at https://github.com/DrMermaid-MichelleGilbert/Satanoperca-daemon-2022.
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The authors would like to thank the Natural History Collections at the University of Massachusetts Amherst for funding. Albertson lab members for support and feedback. Two anonymous reviewers who provided helpful comments that improved the quality of this manuscript.
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Gilbert, M.C., Piggott, S.N. & Albertson, R.C. Substrate type induces plastic responses in the craniofacial morphology of a winnowing cichlid. Hydrobiologia 850, 2241–2255 (2023). https://doi.org/10.1007/s10750-022-05066-6
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DOI: https://doi.org/10.1007/s10750-022-05066-6