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Bacterial community composition and carbon metabolism in a subtropical riverscape

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Abstract

To better understand the interaction between the abundance and composition of bacterial communities, physicochemical conditions, and bacterial metabolic function, we studied the patterns of bacterial community composition and metabolic function in relation to nutrients, land use, and network position in a large subtropical river. Composition varied across the watershed, and with variation in nutrients in particular. Although these changes were partially driven by overall abundance, bacterial groups responded to nutrients differently across the watershed. Most groups were correlated with suspended particulate materials, yet, abundance of β-proteobacteria was highest with elevated nitrate, and abundance of Actinobacteria was highest with elevated soluble reactive phosphate. Land use weakly influenced composition. Landscape position influenced composition, with downstream mainstem riverine sites differing from more upstream and tributary sites. Both production and respiration were influenced by temperature and organic carbon, but we found that production and growth efficiency were not directly related. Production peaked at intermediate levels of abundance, but no groups were correlated with production. Respiration by free-floating bacteria was positively correlated with proportional abundance of α-proteobacteria, γ-proteobacteria, and Actinobacteria. Finally, we found that land use was a relatively weak influence on community composition, but that variation in nutrients was relatively important for both composition and function.

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Acknowledgements

We thank Alexandra Smith, Alisa Abuzeineh, Ben Labay, Chad Thomas, Cori Schwartz, David Hambright, Frances Lash, Josh Perkin, Katheryn Gilson, Kristen Epp, Matthew Chumchal, Mario Sullivan, Robert Maxwell, Timothy Bonner, and Yixin Zhang for their help and support for this project. We thank the Bernot labs at Ball State University for an early review of this manuscript. We thank three anonymous reviewers for their time and input that helped improve the manuscript. The Nature Conservancy, the Houston Endowment Inc., and the Brazos River Authority provided funding for this project. Additional support was provided by National Science Foundation grant DGE-0742306 to W. Nowlin, T. Bonner, and J. Becker, as well as the Fred & Yetta Richan Aquatic Biology Award and H.D. Schulze scholarships to J. Becker.

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  1. Jesse C. Becker

    Present address: Division of Fish and Wildlife, New York State Department of Environmental Conservation, Albany, NY, 12233-4756, USA

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  1. Department of Biology, Texas State University, San Marcos, TX, 78666, USA

    Jesse C. Becker, Kelly J. Rodibaugh, Dittmar Hahn & Weston H. Nowlin

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Becker, J.C., Rodibaugh, K.J., Hahn, D. et al. Bacterial community composition and carbon metabolism in a subtropical riverscape. Hydrobiologia 792, 209–226 (2017). https://doi.org/10.1007/s10750-016-3058-2

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