Abstract
Histone lactylation on its lysine (K) residues has been reported to have indispensable roles in lung fibrosis, embryogenesis, neural development, inflammation, and tumors. However, little is known about the lactylation activity towards histone lysine residue during tooth development. We investigated the dynamic patterns of lactate-derived histone lysine lactylation (Kla) using a pan-Kla antibody during murine tooth development, including lower first molar and lower incisor. The results showed that pan-Kla exhibited temporo-spatial patterns in both dental epithelium and mesenchyme cells during development. Notably, pan-Kla was identified in primary enamel knot (PEK), stratum intermedium (SI), stellate reticulum (SR), dental follicle cells, odontoblasts, ameloblasts, proliferating cells in dental mesenchyme, as well as osteoblasts around the tooth germ. More importantly, pan-Kla was also identified to be co-localized with neurofilament during tooth development, suggesting histone lysine lactylation may be involved in neural invasion during tooth development. These findings suggest that histone lysine lactylation may play important roles in regulating tooth development.
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Abbreviations
- Kla:
-
histone lysine lactylation
- K:
-
lysine
- IEE:
-
inner enamel epithelium
- SIRT1-3:
-
silent information regulator1-3
- HDAC1-3:
-
histone deacetylase 1–3
- EDTA:
-
ethylenediamine tetra-acetic acid
- PBS:
-
phosphate buffered saline
- BSA:
-
bovine serum albumin
- OEE:
-
outer enamel epithelium
- SI:
-
stratum intermedium
- SR:
-
stellate reticulum
- PEK:
-
primary enamel knot
- LaCL:
-
labial cervical loop
- LiCL:
-
lingual cervical loop
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Acknowledgements
This work was supported by the grants from the National Natural Science Foundation of China (82170915 and 31500788) to Dr. Jingwen Yang, the Key project of National Natural Science Foundation of China (82230029) to Dr. Zhi Chen.
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Conceptualization: Y.M., J.Y.; methodology: M.L. and X.Z.; formal analysis: M.L., Q.Y., and J.Y.; resources: Z.C., J.Y.; funding acquisition: Z.C., J.Y.; original manuscript draft preparation: Q.Y., M.L.; writing, review and editing: Q.Y., Y.M., Z.C., J.Y.; supervision: Z.C., J.Y. All authors have read and agreed to the published version of the manuscript.
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Liu, M., Yang, Q., Zuo, H. et al. Dynamic patterns of histone lactylation during early tooth development in mice. J Mol Histol 54, 665–673 (2023). https://doi.org/10.1007/s10735-023-10154-5
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DOI: https://doi.org/10.1007/s10735-023-10154-5