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Developmental changes and regional localization of Dspp, Mepe, Mimecan and Versican in postnatal developing mouse teeth

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Abstract

It has been implicated noncollagenous proteins act as important regulators during odontogenesis. To test the hypothesis that the roles of Dspp, Mepe, Versican and Mimecan in the regulation of odontogenesis may be complementary, comparative investigations on the localization of four proteins were performed by immunohistochemical staining using mouse first molar at different developmental stages as a model. In postnatal 1- day-old mice, all the proteins, excluding Mepe, showed co-expression in young odontoblasts. At postnatal 3, strong immunoreactions for all proteins were detected in odontoblasts. Interestingly, Mepe was present within both cytoplasm and nucleus in odontoblasts. In mice older than 5 days, the expression of Dspp, Mimecan and Versican accumulated in subodontoblastic layer of the coronal pulp at high levels while the co-expression of Mepe and Mimecan significantly existed in predentin. The temporal-spatial specific pattern and unique co-localization of Dspp, Mepe, Mimecan and Versican suggest they play complementary roles during odontogenesis.

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Acknowledgments

We are grateful to Dr. Larry Fisher (National Institute of Dental and Craniofacial Research, Maryland, USA) for providing the antiserums of Dspp, Mepe, Mimecan and Versican. This study was supported by Natural Science Foundation of Shandong Province of China (ZR2010HM050). The authors report no conflicts of interest related to this study.

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Author notes

  1. Z. X. Liu

    Present address: Weifang Medical College, 7166# West Baotong Street, Weifang, 261053, Shandong, People’s Republic of China

  2. K. L. Tang

    Present address: Department of Endodontics, Jinan Stomatological Hospital, 101# Jingliu Road, Jinan, 250001, Shandong, People’s Republic of China

  3. J. Wang

    Present address: Department of Endodontics, Weifang Stomatological Hospital, 325# West Shengli Street, Weifang, 261011, Shandong, People’s Republic of China

Authors and Affiliations

  1. Shandong Provincial Key Laboratory of Oral Biomedicine, Department of Periodontology, School of Stomatology Shandong University, 44-1# West Wenhua Road, Jinan, 250012, Shandong, People’s Republic of China

    C. Hou, Z. X. Liu, K. L. Tang, J. Sun, J. Wang & S. Li

  2. Department of Stomatology, Jinan Central Hospital, 105# Jiefang Road, Jinan, 250013, Shandong, People’s Republic of China

    M. G. Wang

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  1. C. Hou
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Correspondence to S. Li.

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Hou, C., Liu, Z.X., Tang, K.L. et al. Developmental changes and regional localization of Dspp, Mepe, Mimecan and Versican in postnatal developing mouse teeth. J Mol Hist 43, 9–16 (2012). https://doi.org/10.1007/s10735-011-9368-9

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  • DOI: https://doi.org/10.1007/s10735-011-9368-9

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