Abstract
Inpp5f-v3 is a transcriptional variant of Inpp5f (inositol polyphosphate-5-phosphatase F) and locates in distal mouse chromosome 7. It is a paternally expressed imprinted gene in mouse. In this study, we examined the spatiotemporal patterns of Inpp5f-v3 gene during the mouse development. The northern blotting analysis revealed that only one transcript approx 2.7 kb of Inpp5f-v3 was detected in brain. The signals were only observed in brain by the whole-mount in situ hybridization at embryonic day 11.5 (E11.5). The results of quantitative real-time PCR (QRT-PCR) showed that the expression of Inpp5f-v3 increased gradually from the E11.5 to E17.5 and reached the highest at E17.5, then decreased at E18.5 during the brain development. Inpp5f-v3 gene was strongly expressed in the cerebral cortex, olfactory bulb, external germinal layer of cerebellum and ventricular zone (Vz) during the embryonic development (E15.5–E19.5), whereas the expression increased in the olfactory bulb and the cerebellum after birth by using in situ hybridization. The results also demonstrated that the expression of Inpp5f-v3 gene mainly located in olfactory bulb and hippocampus at postnatal day 7 (P7) and adulthood. These results suggest that Inpp5f-v3 is specifically expressed in mouse brain, and may function in the development of mouse brain.
Similar content being viewed by others
References
Arai Y, Ijuin T, Itoh M, Takenawa T, Takashima S, Becker LE (2001) Developmental changes of synaptojanin expression in the human cerebrum and cerebellum. Brain Res Dev Brain Res 129(1):1–9. doi:S0165380601001407
Barlow DP (1995) Gametic imprinting in mammals. Science 270(5242):1610–1613
Choi JD, Underkoffler LA, Wood AJ, Collins JN, Williams PT, Golden JA, Schuster EF Jr, Loomes KM, Oakey RJ (2005) A novel variant of Inpp5f is imprinted in brain, and its expression is correlated with differential methylation of an internal CpG island. Mol Cell Biol 25(13):5514–5522. doi:10.1128/MCB.25.13.5514-5522.2005
Cremona O, Di Paolo G, Wenk MR, Luthi A, Kim WT, Takei K, Daniell L, Nemoto Y, Shears SB, Flavell RA, McCormick DA, De Camilli P (1999) Essential role of phosphoinositide metabolism in synaptic vesicle recycling. Cell 99(2):179–188. doi:S0092-8674(00)81649-9
Dorn GW 2nd, Force T (2005) Protein kinase cascades in the regulation of cardiac hypertrophy. J Clin Invest 115(3):527–537. doi:10.1172/JCI24178
Fishell G, Hatten ME (1991) Astrotactin provides a receptor system for CNS neuronal migration. Development 113(3):755–765
Fowden AL, Sibley C, Reik W, Constancia M (2006) Imprinted genes, placental development and fetal growth. Horm Res 65(Suppl 3):50–58. doi:10.1159/000091506
Goldowitz D, Hamre K (1998) The cells and molecules that make a cerebellum. Trends Neurosci 21(9):375–382. doi:S0166-2236(98)01313-7
Guillemot F, Caspary T, Tilghman SM, Copeland NG, Gilbert DJ, Jenkins NA, Anderson DJ, Joyner AL, Rossant J, Nagy A (1995) Genomic imprinting of Mash2, a mouse gene required for trophoblast development. Nat Genet 9(3):235–242. doi:10.1038/ng0395-235
Hughes WE, Cooke FT, Parker PJ (2000) Sac phosphatase domain proteins. Biochem J 350(Pt 2):337–352
Komuro H, Rakic P (1998) Distinct modes of neuronal migration in different domains of developing cerebellar cortex. J Neurosci 18(4):1478–1490
Minagawa T, Ijuin T, Mochizuki Y, Takenawa T (2001) Identification and characterization of a sac domain-containing phosphoinositide 5-phosphatase. J Biol Chem 276(25):22011–22015. doi:10.1074/jbc.M101579200M101579200
Moorman AF, Houweling AC, de Boer PA, Christoffels VM (2001) Sensitive nonradioactive detection of mRNA in tissue sections: novel application of the whole-mount in situ hybridization protocol. J Histochem Cytochem 49(1):1–8
Ooms LM, Horan KA, Rahman P, Seaton G, Gurung R, Kethesparan DS, Mitchell CA (2009) The role of the inositol polyphosphate 5-phosphatases in cellular function and human disease. Biochem J 419(1):29–49. doi:10.1042/BJ20081673
Piette D, Hendrickx M, Willems E, Kemp CR, Leyns L (2008) An optimized procedure for whole-mount in situ hybridization on mouse embryos and embryoid bodies. Nat Protoc 3(7):1194–1201. doi:10.1038/nprot.2008.103
Reik W, Walter J (2001) Genomic imprinting: parental influence on the genome. Nat Rev Genet 2(1):21–32. doi:10.1038/35047554
Solecki DJ, Govek EE, Tomoda T, Hatten ME (2006) Neuronal polarity in CNS development. Genes Dev 20(19):2639–2647. doi:10.1101/gad.1462506
Sritanaudomchai H, Ma H, Clepper L, Gokhale S, Bogan R, Hennebold J, Wolf D, Mitalipov S (2010) Discovery of a novel imprinted gene by transcriptional analysis of parthenogenetic embryonic stem cells. Hum Reprod 25(8):1927–1941. doi:10.1093/humrep/deq144
Wan YS, Wang ZQ, Shao Y, Voorhees JJ, Fisher GJ (2001) Ultraviolet irradiation activates PI 3-kinase/AKT survival pathway via EGF receptors in human skin in vivo. Int J Oncol 18(3):461–466
Wang VY, Zoghbi HY (2001) Genetic regulation of cerebellar development. Nat Rev Neurosci 2(7):484–491. doi:10.1038/3508155835081558
Wilkinson DG, Nieto MA (1993) Detection of messenger RNA by in situ hybridization to tissue sections and whole mounts. Methods Enzymol 225:361–373
Wood AJ, Bourc’his D, Bestor TH, Oakey RJ (2007) Allele-specific demethylation at an imprinted mammalian promoter. Nucleic Acids Res 35(20):7031–7039. doi:10.1093/nar/gkm742
Zhou QY, Huang JN, Zhu MJ, Zhao SH (2009) Molecular characterization and association analysis with production traits of the porcine INPP5F gene. Mol Biol Rep 36(5):1095–1098. doi:10.1007/s11033-008-9283-7
Zhu W, Trivedi CM, Zhou D, Yuan L, Lu MM, Epstein JA (2009) Inpp5f is a polyphosphoinositide phosphatase that regulates cardiac hypertrophic responsiveness. Circ Res 105(12):1240–1247. doi:10.1161/CIRCRESAHA.109.208785
Acknowledgments
This work was supported from the National Natural Science Foundation of China (No. 30971645) and Heilongjiang Province Technological Project Program Returning Foundation (No. LC08C05).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Yan, C., Hongjuan, H., Yanjiang, X. et al. Expression patterns of imprinted gene Inpp5f-v3 during mouse brain development. J Mol Hist 42, 167–173 (2011). https://doi.org/10.1007/s10735-011-9321-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10735-011-9321-y