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Rat forming incisor requires a rigorous ECM remodeling modulated by MMP/RECK balance

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Abstract

Reversion-inducing-cysteine-rich protein with Kazal motifs (RECK) is a single membrane-anchored MMP-regulator and regulates matrix metalloproteinases (MMP) 2, 9 and 14. In turn, MMPs are endopeptidases that play a pivotal role in remodeling ECM. In this work, we decided to evaluate expression pattern of RECK in growing rat incisor during, specifically focusing out amelogenesis process. Based on different kinds of ameloblasts, our results showed that RECK expression was conducted by secretory and post-secretory ameloblasts. At the secretory phase, RECK was localized in the infra-nuclear region of the ameloblast, outer epithelium, near blood vessels, and in the stellate reticulum. From the transition to the maturation phases, RECK was strongly expressed by non-epithelial immuno-competent cells (macrophages and/or dendritic-like cells) in the papillary layer. From the transition to the maturation stage, RECK expression was increased. RECK mRNA was amplified by RT-PCR from whole enamel organ. Here, we verified the presence of RECK mRNA during all stages of amelogenesis. These events were governed by ameloblasts and by non-epithelial cells residents in the enamel organ. Concluding, we found differential expression of MMPs-2, -9 and RECK in the different phases of amelogenesis, suggesting that the tissue remodeling is rigorously controlled during dental mineralization.

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Acknowledgments

The authors are grateful to the technical assistance of Daniele Ceolin, Gustavo N Zanelatto and Tânia M. Cestari. Special thanks to Dr. Professor Victor Arana-Chavez (Sao Paulo Dental School-USP) for helpful assistance in dissection of enamel organ. This work was supported by grants from the FAPESP (08/53003-9, 07/04148-1, 07/00216-2, 99/10655-5; 01/10707-7 and 07/04148-1), CAPES—(BEX 2415/04-6), and CNPq (475721/2003-9).

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Authors and Affiliations

  1. Laboratory of Molecular Pathology, Department of Oral Pathology, Dental School, University of São Paulo, Sao Paulo, SP, Brazil

    Katiucia Batista Silva Paiva & Fabio Daumas Nunes

  2. Laboratory of Signaling Transduction, Department of Biochemistry, Institute of Biology, State University of Campinas, Campinas, SP, Brazil

    Willian Fernando Zambuzzi

  3. Department of Restorative Dentistry, Piracicaba Dental School, Campinas University, Piracicaba, SP, Brazil

    Thais Accorsi-Mendonça

  4. Department of Biological Sciences, Bauru Dental School, University of São Paulo, Bauru, Brazil

    Rumio Taga

  5. Department of Biochemistry, Institute of Chemistry, University of São Paulo, Sao Paulo, SP, Brazil

    Mari Cleide Sogayar

  6. Cell and Molecular Therapy Center, University of São Paulo, Sao Paulo, SP, Brazil

    Mari Cleide Sogayar & José Mauro Granjeiro

  7. Department of Cell and Molecular Biology, Institute of Biology, Fluminense Federal University, Outeiro de São João Baptista s/n, Campus do Valonguinho, Centro/Niterói, RJ, 24020-150, Brazil

    José Mauro Granjeiro

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  1. Katiucia Batista Silva Paiva
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Correspondence to José Mauro Granjeiro.

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Paiva, K.B.S., Zambuzzi, W.F., Accorsi-Mendonça, T. et al. Rat forming incisor requires a rigorous ECM remodeling modulated by MMP/RECK balance. J Mol Hist 40, 201–207 (2009). https://doi.org/10.1007/s10735-009-9231-4

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  • DOI: https://doi.org/10.1007/s10735-009-9231-4

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