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The MMP activity in developing rat molar roots and incisors demonstrated by in situ zymography

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Abstract

Matrix metalloproteinases (MMPs) have been expressed during root development and periodontal tissue formation, whereas it is not known if these MMP molecules are enzymatically active to degrade the extracellular matrices (ECMs). The present study was designed to investigate the gelatinolytic and collagenolytic activity in rat molar root and incisor development. Three-week old rat mandibles were frozen and cut without fixation or decalcification and processed for in situ zymography using substrates gelatin and collagen. The enzymatic activity was assessed according to the intensity of fluorescence due to the lysis of the substrates. Odontoblasts, predentin, cementum, bone and the enamel matrix showed the high activity. The present study demonstrated MMP activity in calcified tissues using in situ zymography for the first time and the possible involvement of the MMP activity in molar root and incisor development and periodontal tissue formation.

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Acknowledgements

This work was supported in part by grants-in-aid (15390550, 16500285, 17659573, 17791296, 17791508) from the Ministry of Education, Science, Sports and Culture of Japan.

We wish to thank Mr. Masami Eguchi and Mr. Yasuto Mikami, Division of Craniofacial Development and Regeneration, Tohoku University Graduate School of Dentistry, for their excellent assistance in this study.

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Authors and Affiliations

  1. Division of Pediatric Dentistry, Tohoku University Graduate School of Dentistry, Sendai, 980-8575, Japan

    Ichiro Sakuraba, Yuji Hatakeyama & Hideaki Mayanagi

  2. Division of Craniofacial Development and Regeneration, Tohoku University Graduate School of Dentistry, Sendai, Japan

    Ichiro Sakuraba, Junko Hatakeyama & Yasuyuki Sasano

  3. Division of Orthodontic and Dentofacial Orthopedics, Tohoku University Graduate School of Dentistry, Sendai, 980-8575, Japan

    Ichiro Takahashi

Authors
  1. Ichiro Sakuraba
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  2. Junko Hatakeyama
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  3. Yuji Hatakeyama
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  4. Ichiro Takahashi
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  5. Hideaki Mayanagi
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  6. Yasuyuki Sasano
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Correspondence to Yasuyuki Sasano.

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Sakuraba, I., Hatakeyama, J., Hatakeyama, Y. et al. The MMP activity in developing rat molar roots and incisors demonstrated by in situ zymography. J Mol Hist 37, 87–93 (2006). https://doi.org/10.1007/s10735-006-9037-6

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  • DOI: https://doi.org/10.1007/s10735-006-9037-6

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