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The influence of plectin deficiency on stability of cytokeratin18 in hepatocellular carcinoma

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Abstract

Intermediate filaments are important in building the cellular architecture. Previously we found cytokeratin18 was modulated in human hepatocellular carcinoma. Plectin is a cross-linking protein that organizes the cytoskeleton into a stable meshwork, which can maintain the uniform size and shape of hepatocytes. Because the cells of hepatocellular carcinoma were morphologically different from the hepatocytes, we speculated that expression of plectin and organization of intermediate filament might play roles in the pleomorphism of hepatocellular carcinoma cells. In this paper, we studied the plectin expression of hepatocellular carcinoma and liver tissues by immunohistochemistry and immunoblot. The results revealed that plectin was deficient and cytokeratin18 was modulated in hepatocellular carcinoma. Furthermore, we knockdown the plectin mRNA in Chang cells, the result revealed the plectin was deficient and the organization of cytokeratin18 was altered. Conclusively, this study offers a hypothesis that plectin deficient might play an important role in the tumorigenesis of hepatocellular carcinoma.

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Acknowledgments

This work was partially supported by a National Science Council grant NSC94-2320-B-0400-015.

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Authors and Affiliations

  1. Institute of Medicine of Chung Shan Medical University, Taichung, Taiwan

    Chiung-Chi Cheng

  2. Department of pathology, Jen Ai Hospital, Taichung, Taiwan

    Yi-Hsiang Liu & Ren-Jeng Pei

  3. Department of Pathology, Tzu Chi Hospital and University, Hualien, Taiwan

    Yi-Hsiang Liu & Yung-Hsiang Hsu

  4. Department of Pathology, Hospital and Medical College of Chung Shan Medical University, 110, Section 1, Chien-Kuo North Road, Taichung, 408, Taiwan

    Yi-Hsiang Liu, Ren-Jeng Pei, Ming-Chuang Tsai & Yih-Shyong Lai

  5. Department of Nursing, Central Taiwan University of Science and Technology, Taichung, Taiwan

    Chin-Chin Ho

  6. Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX, 77054, USA

    Wei-Ting Chao

  7. Department of Pathology, Changhua Christian Hospital, Changhua, Taiwan

    Kun-Tu Yeh

  8. Department of Surgery, Hospital and Medical College of Chung Shan Medical University, Taichung, Taiwan

    Lu-Chang Ho

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  1. Chiung-Chi Cheng
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  7. Kun-Tu Yeh
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  10. Yih-Shyong Lai
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Corresponding author

Correspondence to Yih-Shyong Lai.

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Dr. Yi-Hsiang Liu contributed equally as the first author.

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Cheng, CC., Liu, YH., Ho, CC. et al. The influence of plectin deficiency on stability of cytokeratin18 in hepatocellular carcinoma. J Mol Hist 39, 209–216 (2008). https://doi.org/10.1007/s10735-007-9155-9

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  • DOI: https://doi.org/10.1007/s10735-007-9155-9

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