Skip to main content
SpringerLink
Log in

Identification and expression analysis of Jr4CLs gene family based on transcriptome and physiological data in walnut (Juglans regia)

  • Original Paper
  • Published:
Plant Growth Regulation Aims and scope Submit manuscript

Abstract

Shoot shriveling severely threaten growth and development of deciduous trees in northern hemisphere, its essence is imbalance of water absorption and evaporation in the branches. In this study, the physiological characteristics of ‘Xiangling’ and ‘Liaohe (Liaoning No. 4)’ during the overwintering process were studied, and key overwintering periods were selected for transcriptome analysis. The results showed that plant hormone metabolism, wax metabolism and lignin metabolism were significantly enriched during the overwintering process. The expression of 4CL, a gene related to lignin metabolism, was significantly up-regulated. We used bioinformatics to analyze the 4CL family and study its expression under drought stress, and finally screened out a highly expressed gene (Jr4CL44) for subsequent functional verification. Overexpression of Jr4CL44 can effectively remove the reactive oxygen species produced by drought stress, increase lignin content and up-regulate the expression of related genes to improve the drought resistance of Arabidopsis thaliana. These results indicate that Jr4CL44 plays an important role in plant resistance to drought stress, which lays a foundation for further study on the functions in practice.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10

Similar content being viewed by others

References

  • Ahmed AAM, Mohamed EA, Hussein MY, Sallam A (2021) Genomic regions associated with leaf wilting traits under drought stress in spring wheat at the seedling stage revealed by GWAS. Environ Exp Bot 184:104393

    Article  CAS  Google Scholar 

  • Banzouzi BVJ, Chong PF, Jia XY, Lu WT, Tin YL (2022) Effects of foliar-spraying nitric oxide on the carbon metabolism enzymes activities and nutrients in leaves and roots of Reaumuria soongorica (pall.) maxim seedlings under NaCl stress. Plant Stress 5:80–92

    Google Scholar 

  • Böttner L, Grabe V, Gablenz S, Böhme N, Appenroth KJ, Gershenzon J, Huber M (2020) Differential localization of flavonoid glucosides in an aquatic plant implicates different functions under abiotic stress. Plant Cell Environ 44:900–914

    Article  PubMed  Google Scholar 

  • Chen RZ, Deng YW, Ding YL, Guo JX, Qiu J, Wang B, Li JY (2022) Rice functional genomics: decades’ efforts and roads ahead. Sci China (life Sciences) 65:33–92

    Article  PubMed  Google Scholar 

  • Choimaa D, Gurbazar YB, Shagjjav O (2023) Non-structural carbohydrate concentrations in contrasting dry and wet years in early- and late-successional boreal forest trees. Trees 37:1315–1332

    Article  Google Scholar 

  • Chun HJ, Lim LH, Cheong MS, Baek D, Park MS, Mi CH, Kim MC (2021) Arabidopsis CCoAOMT1 plays a role in drought stress response via ROS-and ABA-dependent manners. Plants 10:831

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Dampanaboina L, Yuan N, Mendu V (2021) Estimation of plant biomass lignin content using thioglycolic acid (TGA). J o V E. https://doi.org/10.3791/62055

    Article  Google Scholar 

  • Daniel A, Matthew S, Corina N (2023) Epigenomic and transcriptomic alterations in deep-layer pyramidal neurons of individuals who died by suicide with histories of early-life adversity. Biol Psychiat 93(9S):S38–S39

    Google Scholar 

  • Dong XC, Jiang CC, Wei SW, Jiao HJ, Ran K, Dong R, Wang SM (2022) The regulation of plant lignin biosynthesis under boron deficiency conditions. Physiol Plant 174:e13815

    Article  CAS  PubMed  Google Scholar 

  • Doron L, Xu LX, Rachmilevitch S, Stern DB (2020) Transgenic overexpression of rubisco subunits and the assembly factor RAF1 are beneficial to recovery from drought stress in maize. Environ Exp Bot. https://doi.org/10.1016/j.envexpbot.2020.104126

    Article  Google Scholar 

  • Erturk U, Solak AO, Yucel S, Borkoyev B (2020) Determination of nut properties and chemical compositions of walunt (Juglans regia L.) genotypes grown in genotypes grown in walnut-fruit forests of Kyrgyzstan. Fresenius Environ Bull 29:2809–2817

    CAS  Google Scholar 

  • Gao YL, Zhang ZX, Wang SC, Ma NY, Wang YX (2023) Transcriptome analysis reveals wax and phytohormone metabolism potentially involved in shooting shrivelling of apple branches overwinter. Funct Plant Biol. https://doi.org/10.1071/FP22283

    Article  PubMed  Google Scholar 

  • Gao HZ, Jia ZG, Zhang L (2024) Comprehensive evaluation of the resistance to shoot shriveling before and after sprouting in different varieties of Ping’ou hybrid hazelnut. Front for Glob Chang. https://doi.org/10.3389/ffgc.2023.1304271

    Article  Google Scholar 

  • Geng JJ, Liu JH (2019) Supplementary data for the transcription factor Csbhlh18 of sweet orange (Citrus sinensis) functions in modulation of cold tolerance and reactive oxygen species homeostasis by regulating the antioxidant gene. J Exp Bot 69:2677–2692

    Article  Google Scholar 

  • Gholami M, Tijburg MJ, Schuur B (2024) Continuous liquid–liquid extraction to recover lignin and furanics from lactic acid: choline chloride deep eutectic solvent after cooking of spruce. Sep Purif Technol. https://doi.org/10.1016/j.seppur.2024.126526

    Article  Google Scholar 

  • Gu HL, Wang Y, Xie H, Qiu C, Zhang SN, Xiao J, Ding ZT (2020) Drought stress triggers proteomic changes involving lignin, flavonoids and fatty acids in tea plants. Sci Rep 10:15504

    Article  PubMed  PubMed Central  Google Scholar 

  • Guan SX, Xu Q, Ma DR, Zhang WZ, Xu ZJ, Zhao MH, Guo ZF (2019) Transcriptomics profiling in response to cold stress in cultivated rice and weedy rice. Gene 685:96–105

    Article  CAS  PubMed  Google Scholar 

  • He S, Li B, Wang H, Liang SC, Ding ZT, Wang Y, Qian WJ (2023) Characterization of invertase inhibitors (InvInhs) in tea plant, and their potential roles in participating in growth, development and cold response. Sci Hortic 308:23–40

    Article  Google Scholar 

  • Ji XY, Tang JL, Zheng X, Zhang JP (2024) The regulating mechanism of salt tolerance of black walnut seedlings was revealed by the physiological and biochemical integration analysis. Plant Physiol Biochem 210:108548

    Article  CAS  PubMed  Google Scholar 

  • Kaur J, Tiwari N, Asif HM (2024) Integrated genome-transcriptome analysis unveiled the mechanism of Debaryomyces hansenii-mediated arsenic stress amelioration in rice. J Hazard Mater. https://doi.org/10.1016/j.jhazmat.2024.133954

    Article  PubMed  Google Scholar 

  • Khan H, Ullah I, Zeb U, Zhao P (2021) Identification, characterization and expression profiles of dof transcription factors in common walnut (Juglans regia L.). Pol J Environ Stud 30:1–12

    Article  Google Scholar 

  • Kim SE, Lee CJ, Ji CY, Kwak SS (2019) Transgenic sweet `potato plants overexpressing tocopherol cyclase display enhanced α-tocopherol content and abiotic stress tolerance. Plant Physiol Biochem 144:436–444

    Article  CAS  PubMed  Google Scholar 

  • Kwon HJ, Nam YE, Seok k, (2022) Comparative carbohydrate metabolism in the shoots of a cold-hardy and a cold-sensitive peach (Prunus persica) cultivar during cold acclimation and deacclimation. Hortic Environ Biotechnol 63:39–53

    Article  CAS  Google Scholar 

  • Li CN, Dong FX, Wang GX (2010) Study on the tolerance and critical water capacity of shoot shriveling in hybrid hazelnuts. J Res for Sci 23:330–335

    Google Scholar 

  • Li HB, Li N, Yang SZ, Peng HZ, Wang LL, Wang Y, Gao ZH (2017) Transcriptomic analysis of Casuarina equisetifolia L. in responses to cold stress. Tree Genet Genomes 13:7–7

    Article  Google Scholar 

  • Li JJ, Lin ZC, Zhang YL, Gao HN, Wang HB, Jiang H, Li YY (2022) An apple long-chain acyl-CoA synthase, MdLACS1, enhances biotic and abiotic stress resistance in plants. Plant Physiol Biochem 297:110597

    Google Scholar 

  • Li XL, Meng D, Li MJ, Zhou J, Yang YZ (2023) Transcription factors MhDREB2A/MhZAT10 play a role in drought and cold stress response crosstalk in apple. Plant Physiol 192(3):2203–2220

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Liang GP, Li YM, Wang P, Jiao SZ, Wang H, Mao J, Chen BH (2022) VaAPL1 promotes starch synthesis to constantly contribute to soluble sugar accumulation, improving low temperature tolerance in Arabidopsis and tomato. Front Plant Sci 13:920424

    Article  PubMed  PubMed Central  Google Scholar 

  • Lin SZ, Zhang ZY, Lin YZ, Zhang L, Guo H (2014) The role of calcium and calmodulin in freezing-induced freezing resistance of Populus tomentosa cuttings. J Plant Physiol Mol Biol 30:59–68

    Google Scholar 

  • Liu WP, Su SC, Liu X, Hou ZX (2012) Comparison of different cultivars of blueberry overwintering ability in Qingdao of China. Am J Plant Sci 3:391–396

    Article  CAS  Google Scholar 

  • Liu WH, Cheng CZ, Chen FL, Ni SS, Lin YL, Lai ZX (2018) High-throughput sequencing of small RNAs revealed the diversified cold-responsive pathways during cold stress in the wild banana (Musa itinerans). BMC Plant Biol 18:308–308

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Melicher P, Dvorak P, Samaj J, To T (2022) Protein-protein interactions in plant antioxidant defense. Front Plant Sci. https://doi.org/10.3389/fpls.2022.1035573

    Article  PubMed  PubMed Central  Google Scholar 

  • Mondal SK, Roy S (2018) Genome-wide sequential, evolutionary, organizational and expression analyses of phenylpropanoid biosynthesis associated MYB domain transcription factors in Arabidopsis. J Biomol Struct Dyn 6:1577–1601

    Article  Google Scholar 

  • Qu DH, Wu FL, Yang J, Li MJ, Yang LN, Xie RX, Su HY (2023) Transcription factor PtNAC101 negatively regulates the lignin synthesis and salt tolerance in Populus trichocarpa. Environ Exp Bot 205:105149

    Article  CAS  Google Scholar 

  • Samia F, Shah AA, Naveed NH, Nijabat A, Yasin NA, Batool AI, Ali A (2021) Synergistic application of silver nanoparticles and indole acetic acid alleviate cadmium induced stress and improve growth of Daucus carota L. Chemosphere 290:133200

    Google Scholar 

  • Tomotaka S, Kazunori H, Shinichi O, Akiyoshi K (2014) Transcript level analysis of lignin and flavonoid biosynthesis related genes in Eucalyptus globulus. Am J Plant Sci 5:2764–2772

    Article  Google Scholar 

  • Waadt R, Seller CA, Hsu P, Takahashi Y, Munemasa S, Schroeder JI (2022) Publisher correction: plant hormone regulation of abiotic stress responses. Nat Rev Mol Cell Biol 7:23

    Google Scholar 

  • Wadhwa N, Singh D, Yadav R (2023) Role of TRDMT1/DNMT2 in stress adaptation and its influence on transcriptome and proteome dynamics under osmotic stress in Physcomitrium patens. Physiol Plant 175(5):e14014

    Article  CAS  PubMed  Google Scholar 

  • Wang LH, Zhai YN, Wu JX, Stevanato P, Sha SS, Geng G, Wang YG (2022a) Low night temperature-induced feedback inhibition of photosynthesis through sucrose accumulation in sugar beet (Beta vulgaris L.) leaves. Environ Exp Bot 204:55–60

    Article  Google Scholar 

  • Wang L, Qian BL, Zhao L, Liang MH, Zhan XQ, Zhu JH (2022b) Two triacylglycerol lipases are negative regulators of chilling stress tolerance in Arabidopsis. Int J Mol Sci. https://doi.org/10.3390/ijms23063380

    Article  PubMed  PubMed Central  Google Scholar 

  • Wang L, Chen YL, Wu MT, Dai FW, Ye MQ, Chen FP, Huang H (2023) Involvement of lignin deposition and cell wall degradation in stem senescence of Chinese flowering cabbage during storage. Postharvest Biol Technol 198:112256–112256

    Article  CAS  Google Scholar 

  • Waqar K, Manzoor H, Kishwar A, Murad A, Mohammad N (2020) Distribution and phenotypic variation in Juglans regia L. growing in Hindu Kush ranges of Pakistan. Acta Ecol Sin 40:363–372

    Article  Google Scholar 

  • Yang J, Zhang X, Wang D (2024) The deterioration of starch physiochemical and minerals in high-quality indica rice under low-temperature stress during grain filling. Front Plant Sci. https://doi.org/10.3389/fpls.2023.1295003

    Article  PubMed  PubMed Central  Google Scholar 

  • Zhanassova K, Kurmanbayeva A, Gadilgereyeva B, Yermukhambetova R, Iksat N, Amanbayeva U, Masalimov Z (2021) ROS status and antioxidant enzyme activities in response to combined temperature and drought stresses in barley. Acta Physiol Plant 43:1–12

    Article  Google Scholar 

Download references

Funding

This work was supported by Gansu Provincial Forestry ang grassland Bureau from the list of projects (Project Number 2022kj057).

Author information

Authors and Affiliations

  1. College of Horticulture, Gansu Agricultural University, Lanzhou, 730070, China

    Xiao-Lan Ma, Yan-Long Gao, Zhong-Xing Zhang, Xiao-Ya Wang & Yan-Xiu Wang

Authors
  1. Xiao-Lan Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yan-Long Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zhong-Xing Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xiao-Ya Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yan-Xiu Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

XLM and XYW designed the research. XLM and YLG performed the experiments. YLG and ZXZ performed the data analysis and interpretation. XLM and ZXZ prepared the figures and tables. XLM wrote the manuscript. All authors read, commented on and approved the manuscript.

Corresponding author

Correspondence to Yan-Xiu Wang.

Ethics declarations

Competing interest

The authors declare that they have no competing interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ma, XL., Gao, YL., Zhang, ZX. et al. Identification and expression analysis of Jr4CLs gene family based on transcriptome and physiological data in walnut (Juglans regia). Plant Growth Regul (2024). https://doi.org/10.1007/s10725-024-01153-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s10725-024-01153-x

Keywords

Search

Navigation