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ROS status and antioxidant enzyme activities in response to combined temperature and drought stresses in barley

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Abstract

The influence of drought stress on barley (Hordeum vulgare) has been investigated. The experiments were conducted with seedlings of barley—by the application of combined drought and temperature stresses. Here we showed that combination of drought and high temperature inflicted more severe damage to plants than the drought and low temperature stress. The temperature stress triggered more drastic changes in plant morphology/physiology and biochemistry than the drought stress. Interestingly, plants exposed to high temperature exhibited significant reduction of shoot and root aldehyde oxidase (AO) activity. Moreover, increased temperature resulted in lower levels of reactive oxygen species (ROS), while drought stress had an opposite effect on ROS accumulation and AO activity. This is the first demonstration of inhibition of plant AO and ROS in response to heat stress. The combined heat and drought stresses resulted in increased activity of catalase (CAT) and superoxide dismutase (SOD) in roots but not in shoots. Our findings indicate that heat and drought stresses may induce activation of different antioxidant enzymatic defense mechanisms and heat stress significantly affects enzymes responsible for the ROS accumulation in plants.

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Abbreviations

AO:

Aldehyde oxidase

ROS:

Reactive oxygen species

CAT:

Catalase

SOD:

Superoxide dismutase

POD:

Peroxidase

HSP:

Heat shock proteins

ALDH:

Aldehyde dehydrogenases

H2O2 :

Hydrogen peroxide

NBT:

Nitroblue tetrazolium

RWC:

Relative water content

MDA:

Malondialdehyde

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Funding

This work was supported by the Science Committee of the Ministry of Education and Science of the Republic of Kazakhstan under Grant nos. BR05236574, AP05135485.

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Author notes

  1. Kuralay Zhanassova and Assylay Kurmanbayeva contributed equally to this work as first authors.

Authors and Affiliations

  1. Department of Biotechnology and Microbiology, L.N. Gumilyov Eurasian National University, Nur-Sultan, Kazakhstan

    Kuralay Zhanassova, Assylay Kurmanbayeva, Bakhytgul Gadilgereyeva, Roza Yermukhambetova, Nurgul Iksat, Ulbike Amanbayeva, Assemgul Bekturova, Zhanerke Tleukulova, Rustem Omarov & Zhaksylyk Masalimov

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  1. Kuralay Zhanassova
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  2. Assylay Kurmanbayeva
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Correspondence to Assylay Kurmanbayeva.

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Zhanassova, K., Kurmanbayeva, A., Gadilgereyeva, B. et al. ROS status and antioxidant enzyme activities in response to combined temperature and drought stresses in barley. Acta Physiol Plant 43, 114 (2021). https://doi.org/10.1007/s11738-021-03281-7

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