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The LasmiR858-MYB111 module enhances the antioxidant capacity of green leaf lettuce by promoting flavonoid biosynthesis

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Abstract

Flavonoids are secondary metabolites found in plants that possess antioxidant properties. MicroRNAs are small non-coding RNAs that play a crucial role in regulating gene expression and are involved in both developmental and metabolic pathways. The study analysed the differences in indices of lettuce with different leaf colours, a STTM-miR858 silencing vector was constructed using molecular biology techniques, and genetically modified lettuce plants with reduced expression of Las-miR858 were obtained. The interaction mechanism between miR858 and MYB111 was predicted and verified. The study found that reduced expression of miR858 regulated its target MYB111, which subsequently stimulated the expression of key enzyme genes in the flavonoid biosynthesis pathway. This resulted in the accumulation of flavonoids in lettuce, enhancing its antioxidant capacity. This study is the first to explain the regulation of flavonoids by the LasmiR858 network, providing a theoretical and empirical basis and genetic resources for the study of lettuce flavonoid mechanisms.

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Acknowledgements

The authors acknowledge to the Northwest A&F University for providing experimental facilities and the Shaanxi Provincial Department of Agriculture for research funding.

Funding

This work was supported by “Agricultural Science and Technology Innovation Integration Promotion Project of Shaanxi Provincial Department of Agriculture” (NYKJ-2018-YL22 and 2017—TS2).

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Authors and Affiliations

  1. College of Life Sciences, Northwest A&F University, Yangling, Shaan Xi, China

    Xiurong Lv, Yuhui Zhao, Qiuyu Zhao, Li Zhao & Yongjun Wu

  2. College of Horticulture, Northwest A&F University, Shaan Xi, Yangling, China

    Zhenchao Yang

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  1. Xiurong Lv
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  2. Yuhui Zhao
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  4. Li Zhao
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Contributions

The experiment was designed by Yongjun Wu and Zhenchao Yang; the experiments and data analysis were conducted by Yuhui Zhao, Xiurong Lv, and Li Zhao; The manuscript was drafted by Xiurong Lv and Yuhui Zhao, the data was reviewed and edited by Yongjun Wu and Qiuyu Zhao, the manuscript was edited by Yongjun Wu and Zhenchao Yang. All authors have read and approved the final manuscript.

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Correspondence to Yongjun Wu.

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Communicated by Xingfeng Shao.

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Lv, X., Zhao, Y., Zhao, Q. et al. The LasmiR858-MYB111 module enhances the antioxidant capacity of green leaf lettuce by promoting flavonoid biosynthesis. Plant Growth Regul (2024). https://doi.org/10.1007/s10725-024-01136-y

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