Abstract
Short cutting is the main method of tea propagation. The callus formation at the base of cuttings has a certain absorption function. The early callus emergence is conducive to the resistance of cuttings to water and nutrient stress, thus promoting rooting and survival. In order to investigate the effect of light quality on the callus formation at the base of cuttings, this study used white (WL), red (660 nm, RL), and blue (430 nm, BL) light emitting diodes (LEDs) to treat tea cuttings. The results showed that compared with WL, BL promoted callus formation and RL inhibited callus formation. In order to explore the internal molecular mechanism of light quality affecting the difference of cutting callus formation, phytohormone determination and full-length transcriptome sequencing were conducted on mature leaves under different light quality treatments. The analysis of phytohormone showed that the contents of abscisic acid (ABA), indole-3-carboxylic acid (ICA), trans-zeatin (tZ), gibberellin 9 (GA9) and 5-deoxystrigol (5DS) were consistent with the callus formation trend, which showed BL > WL > RL. Full-length transcriptome sequencing was performed on mature leaves of cuttings under darkness (DL, CK), WL, RL and BL. Kyoto encyclopedia of genes and genomes (KEGG) analysis of genes, many genes participated in the “plant hormone signal transduction (ko04075)”, such as auxin early response protein AUX/IAA, auxin response factor ARF, two-component response regulator B-ARR, and DELLA genes had highest expression under BL. Meanwhile, auxin synthesis and transport related genes were analyzed,, including L-tryptophan–pyruvate aminotransferase 1-like TAA1, flavin monooxygenase gene YUCCA, PIN1,3,4 and PIN-LIKES6 and 7, which also had the highest expression under BL. In conclusion, this study revealed the molecular mechanism of light quality regulating the callus formation of tea cutting, and provided new insights for the application of light quality in tea breeding.
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Data availability
The raw data for RNA-seq have been uploaded to the NCBI SRA with accession number PRJNA928604.
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This research was funded by the Technology System of Modern Agricultural Industry in Shandong Province (SDAIT19-01) and the Special Foundation for Distinguished Taishan Scholar of Shandong Province (ts201712057), the Project of Agricultural Science and Technology Fund in Shandong Province (2019LY002, 2019YQ010, and 2019TSLH0802), Shandong Agricultural Seed Improvement Project (2020LZGC010), the Project of Rizhao Natural Science Foundation Youth Fund (RZ2021ZR48). The Livelihood Project of Qingdao City (22-3-7-xdny-5-nsh). The Agricultural Science and Technology Innovation Project of Shandong Academy of Agricultural Sciences (CXGC2023F18, CXGC2023A11). Natural Science Foundation of Shandong Province (ZR2023QC086).
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Yaozong Shen conducted the experiment, analyzed the data, and wrote a manuscript. Xiao Han participated in the manuscript writing and picture production. Zhaotang Ding, Kai Fan and Yu Wang participated in the experimental design and revised the manuscript. Hui Wang conducted the experiment, collected samples and statistical data. Jiazhi Shen revised the manuscript. He Li, Shibo Ding and Dapeng Song collected samples. All authors contributed to the article and approved the submission.
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Shen, Y., Han, X., Wang, H. et al. Full-length transcriptome and metabolism revealed the difference of callus formation of tea cutting under white, red and blue light. Plant Growth Regul 101, 715–726 (2023). https://doi.org/10.1007/s10725-023-01052-7
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DOI: https://doi.org/10.1007/s10725-023-01052-7