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ZmTIFY16, a novel maize TIFY transcription factor gene, promotes root growth and development and enhances drought and salt tolerance in Arabidopsis and Zea mays

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Abstract

The TIFY (a conserved core motif TIF[F/Y]XG) protein is a plant-specific transcription factor that regulates various plant growth/development and stress-related processes. Although many TIFY proteins have been reported in Arabidopsis thaliana, rice (Oryza sativa L.), and other plants, few studies have investigated the role of TIFY proteins in maize (Zea mays L.). In this study, we cloned and performed a preliminary structural and functional analysis of ZmTIFY16, a transcription factor gene that we previously identified in the genome of maize. This gene showed high expression in young and mature leaves and was strongly induced by low temperature (4ºC), dehydration, drought, salt, and abscisic acid. Its heterologous expression in Arabidopsis markedly improved drought and salt tolerance, increased chlorophyll and proline contents, enhanced superoxide dismutase activity, and decreased malondialdehyde content. Moreover, ZmTIFY16 interacted with ZmMYC2, an essential regulator of the jasmonic acid signaling pathway, promoted root growth, and improved salt tolerance in overexpressing maize lines. The results suggest that ZmTIFY16 may be critical for regulating maize growth and abiotic stress responses. ZmTIFY16 may be a novel candidate gene for promoting root growth and development and regulating drought and salt tolerance in maize.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (NSFC) 32001430, the Beijing Natural Science Foundation (No.6222009), the Foundation of Beijing Academy of Agriculture and Forestry Sciences KJCX20200205, KJCX20200407, and KJCX20220402.

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Author notes

  1. Chun Zhang and Ruijia Yang contributed equally to this article.

Authors and Affiliations

  1. Institute of Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, 100097, Beijing, China

    Chun Zhang, Ruijia Yang, Tongtong Zhang, Dengyu Zheng, Xianglong Li, Zhongbao B. Zhang & Zhongyi Y. Wu

  2. College of Life Sciences, Capital Normal University, 100048, Beijing, China

    Ruijia Yang, Tongtong Zhang & Legong G. Li

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  1. Chun Zhang
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Contributions

Chun Zhang: Conceptualization, Methodology, Formal Analysis, Funding Acquisition, Validation, Writing-Review & Editing; Ruijia Yang: Data Curation, Formal Analysis, Visualization, Writing-Original Draft; Tongtong Zhang, Dengyu Zheng and Xianglong Li: Data Curation; Zhongbao Zhang: Conceptualization, Funding Acquisition, Writing-Review & Editing; Legong Li: Writing-Review & Editing; Zhongyi Wu: Conceptualization, Project Administration, Writing-Review & Editing. These authors contributed equally: Chun Zhang, Ruijia Yang

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Correspondence to Zhongbao B. Zhang, Legong G. Li or Zhongyi Y. Wu.

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Zhang, C., Yang, R., Zhang, T. et al. ZmTIFY16, a novel maize TIFY transcription factor gene, promotes root growth and development and enhances drought and salt tolerance in Arabidopsis and Zea mays. Plant Growth Regul 100, 149–160 (2023). https://doi.org/10.1007/s10725-022-00946-2

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