Abstract
To explore the role of jasmonic acid (JA) in seed germination of japonica rice under Cadmium (Cd) stress, the growth indices of japonica rice varieties under the bud stage were measured for identifying the variety difference in Cd tolerance. Then the tolerant variety JJ818 and the sensitive one NJ9108 were treated under 150 μmol L−1 Cd concentration, 0.5 μmol L−1 methyl jasmonate (MeJA), and 10 μmol L−1 Ibuprofen (IBU) alone or in combination to study the molecular mechanism. In addition to the germination phenotype (germination rate, root length and bud length), soluble protein content, soluble sugar content, and sugar components, α-amylase activity, and its related gene expression were also measured. The results showed that Cd treatment restricted the root length of rice seed (P < 0.05) but had little effect on germination rate and shoot length. The combined application of MeJA with Cd treatment further reduced the seed germination rate and shoot length, while its inhibitor (Ibuprofen) treatment showed an opposite trend. Cd treatment also decreased the expression of the αAmy and RL1, and the accompanying increases of the gene expression of JIOsPR10, OsLOX1, and OsLOX4 related to synthesizing JA. The results indicated that JA negatively regulates the of root growth of japonica rice seeds under cadmium treatment via the genes related to the synthesis at the bud stage. Compared with the NJ9108, JJ818 was more tolerant to Cd stress and was related to its insensitivity to JA.
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Acknowledgements
The authors thank the anonymous reviewers and editorial staff for their time and attention. We also apologize if some important references were not cited. This study was supported by grants from National Natural Science Foundation of China international (regional) Cooperation and exchange program (31861143011), National Natural Science Foundation of China (No.31571585), Key RESEARCH and Development Program of Jiangsu Province (Modern Agriculture) (BE2021359), “elect the best candidates to undertake key research projects (Jie Bang Gua Shuai)” for seed industry revitalization of Jiangsu Province [JBGS(2021)038], [JBGS(2021)039].
Funding
This study was supported by grants from National Natural Science Foundation of China international (regional) Cooperation and exchange program (31861143011), National Natural Science Foundation of China (No. 31571585), Key RESEARCH and Development Program of Jiangsu Province (Modern Agriculture) (BE2021359), “elect the best candidates to undertake key research projects (Jie Bang Gua Shuai)” for seed industry revitalization of Jiangsu Province [JBGS(2021)038], [JBGS(2021)039].
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Dr. XL designed research and drafted the entire manuscript. Mr. BW performed research and analyzed data. Material preparation were performed by Dr. XL, Mr. FW and Dr. JY. Data collection were performed by Mr. BW, Miss ZZ and Miss YL. Analysis were performed by Mr. BW and Dr. XL. The first draft of the manuscript was written by BW and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Wu, B., Zeng, Z., Wu, X. et al. Jasmonic acid negatively regulation of root growth in Japonica rice (Oryza sativa L.) under cadmium treatment. Plant Growth Regul 98, 651–667 (2022). https://doi.org/10.1007/s10725-022-00897-8
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DOI: https://doi.org/10.1007/s10725-022-00897-8