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Genome-wide analysis of invertase gene family in wheat (Triticum aestivum L.) indicates involvement of TaCWINVs in pollen development

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A Correction to this article was published on 16 June 2022

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Abstract

Invertase (INV, EC3.2.1.26) is involved in carbohydrate partitioning by irreversibly hydrolyzing sucrose into fructose and glucose. INV genes that are specifically expressed in anthers are closely related to male fertility but they have not been identified in wheat. In this study, we identified 130 INV genes in wheat with uneven distributions on 21 chromosomes. According to their physical and chemical properties, subcellular locations, and phylogenetic analysis, the INV genes were divided into two acidic INV subtypes and two neutral/alkaline INV subtypes. Polyploidy and segmental duplications were found to be mainly responsible for expansion of the wheat INV gene family. Expression profiles in wheat showed that the INV genes had specific temporal and spatial characteristics. Subsequently, TaCWINV40 encoding a cell wall invertase (CWINV) was subjected to further study. Subcellular localization analysis showed that TaCWINV40 was localized in the cell wall. Further phenotypic and cytological analyses of BSMV:TaCWINV40 silenced wheat plants indicated that TaCWINV40 and/or its highly similar duplicates are involved in anther and pollen development. These findings are useful for improving our understanding of INV family members in wheat and they suggest important roles for TaCWINVs in male sterile wheat lines.

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All data generated or analyzed during this study are included in this published article and its supplementary information files.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China [grant number 32072060].

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  1. Jiali Ye and Yaning Bu contributed equally to this work.

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  1. College of Agronomy, Northwest A&F University, Yangling, Shaanxi, China

    Jiali Ye, Yaning Bu, Mengting He, Yongfeng Wu, Xuetong Yang, Lingli Zhang & Xiyue Song

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  1. Jiali Ye
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Jiali Ye, Yaning Bu, Mengting He, Yongfeng Wu, and Xuetong Yang. The first draft of the manuscript was written by Jiali Ye and Yaning Bu, and all authors commented on subsequent versions of the manuscript. Xiyue Song and Lingli Zhang critically revised the manuscript. All authors have read and approved the manuscript.

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Correspondence to Lingli Zhang or Xiyue Song.

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Communicated by Zsófia Bánfalvi.

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Ye, J., Bu, Y., He, M. et al. Genome-wide analysis of invertase gene family in wheat (Triticum aestivum L.) indicates involvement of TaCWINVs in pollen development. Plant Growth Regul 98, 77–89 (2022). https://doi.org/10.1007/s10725-022-00834-9

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