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Transcriptome analysis revealed glucose application affects plant hormone signal transduction pathway in “Red Globe” grape plantlets

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Abstract

Sugars play important roles as signaling molecules in plant growth, development and photosynthetic modulation. The key mechanisms of sugar metabolism and the expression levels of genes involved in these processes are, however, poorly understood. In the present study, we applied different concentrations (0, 1, 2, and 4%) of glucose to “Red Globe” grape plantlets grown in vitro and constructed four libraries designated as G0, G10, G20, and G40 through transcriptomic analyses. We found that the exogenous glucose significantly enhanced photosynthesis and promoted growth and development of the grape plantlets compared with G0. Hormone levels of the plantlets, particularly those of ZT, IAA, ABA, and JA, significantly increased at G20 and G40 in contrast to G0. Through RNA sequencing, 4397 differentially expressed genes, including 875 up-regulated and 3522 down-regulated genes, were identified. Functional analysis revealed that these genes were significantly enriched in plant hormone signal transduction pathway. It is revealed here that AUX1, AUX/IAA, TIR1, ARF, GH3 and SAUR, key genes displayed identical differential expression profiles to auxin transduction pathway, and PYL/PYR, SnRK2s, PP2Cs to abscisic acid, JAZ, MYC2 to jasmonic acid. Furthermore, the grape plantlets had the ability to grow photoautotrophically in G0. LHCAs, PSAs, NDHH and PSBs were associated with photoautotrophic. ACO1 and ACO3 were confirmed to be associated with glucose metabolism. The highest concentration of glucose (G40) reduced photosynthesis of “Red Globe” plantlets due to down-regulation of Rubisco activity, and RH39 was a key gene involved in the regulation of Rubisco activity. This study has revealed that the exogenous glucose enhances photosynthesis and improves plant growth and development of “Red Globe” plantlets by regulating the expression of genes related to photosynthesis and plant hormone signal transduction pathway.

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Acknowledgements

This research was financially supported by National Natural Science Foundation of China (31460499 and 31460500).

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  1. Juan Mao and Wenfang Li have contributed equally to the work.

Authors and Affiliations

  1. College of Horticulture, Gansu Agricultural University, Lanzhou, 730070, People’s Republic of China

    Juan Mao, Wenfang Li, Baoqin Mi, Zonghuan Ma, Mohammed Mujitaba Dawuda, Cunwu Zuo, Xuefeng Jiang & Baihong Chen

  2. Department of Horticulture, FoA, University for Development Studies, P. O. Box TL 1882, Tamale, Ghana

    Mohammed Mujitaba Dawuda

  3. Instrumental Researches and Analysis Center, Gansu Agricultural University, Lanzhou, 730070, Gansu, People’s Republic of China

    Yongmei Zhang

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  1. Juan Mao
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Correspondence to Baihong Chen.

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Mao, J., Li, W., Mi, B. et al. Transcriptome analysis revealed glucose application affects plant hormone signal transduction pathway in “Red Globe” grape plantlets. Plant Growth Regul 84, 45–56 (2018). https://doi.org/10.1007/s10725-017-0320-1

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