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Diverse antioxidant enzyme levels in different sweetpotato root types during storage root formation

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Abstract

Sweetpotato forms different types of root during storage root production, including fibrous roots (FR), thick roots (TR), and storage roots (SR). To understand the functions that antioxidant enzymes play in the development of these different roots, we investigated the activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT), peroxidase (POD), and polyphenol oxidase (PPO). Significantly higher activity levels were observed in FR than in both TR and SR for SOD, APX and CAT. Both POD and PPO activity were significantly higher in FR and TR than in SR. Quantitative RT-PCR analysis was used to investigate antioxidant gene expression patterns in the different stage and root types of sweetpotato. Some genes displayed root-type or stage specific responses depending on the root part. CuZnSOD, APX, swpa7, swpa8, swb6, and swpb7 were expressed at significantly higher levels in the FR and SR than in the TR. CAT, swpa9, swpa4, and swpb7 exhibited significantly higher expression levels in the FR and TR than in the SR. In addition, they also showed higher levels of expression in the lower parts of the FR and TR at the early stage, whereas SR was expressed in the upper part. These results indicate that antioxidant enzymes are differentially regulated during the early stages of sweetpotato root development.

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Abbreviations

APX:

Ascorbate peroxidase

CAT:

Catalase

FR:

Fibrous roots

GPX:

Glutathione peroxidase

GST:

Glutathione-S-transferase

POD:

Peroxidase

PPO:

Polyphenol oxidase

ROS:

Reactive oxygen species

SR:

Storage roots

TR:

Thick roots

TRX:

Thioredoxin

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Acknowledgments

This work was supported by Academy-oriented Research Funds of Development Fund Foundation, Gyeongsang National University, 2013 (GNUDFF-2013-12), Grants from the National Center for GM Crops (PJ008097) and the Systems and Synthetic Agrobiotech Center (PJ009506), the Biogreen21 Project for Next Generation, Rural Development Administration, Korea, and the KRIBB Initiate Program.

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Authors and Affiliations

  1. Department of Biology Education, College of Education, IALS, PMBBRC, Gyeongsang National University, 501 Jinju-Daero, Jinju, 660-701, Korea

    Yun-Hee Kim

  2. Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 111 Gwahangno, Yusong-gu, Daejeon, 305-806, Korea

    Sung-Chul Park, Chang Yoon Ji, Jae Cheol Jeong, Haeng-Soon Lee & Sang-Soo Kwak

  3. Department of Green Chemistry and Environmental Biotechnology, Korea University of Science and Technology (UST), Daejeon, Korea

    Sung-Chul Park, Chang Yoon Ji, Haeng-Soon Lee & Sang-Soo Kwak

  4. Department of Applied Biology, IALS, Gyeongsang National University, Jinju, 660-701, Korea

    Jeung Joo Lee

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  1. Yun-Hee Kim
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Correspondence to Yun-Hee Kim or Sang-Soo Kwak.

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Kim, YH., Park, SC., Ji, C.Y. et al. Diverse antioxidant enzyme levels in different sweetpotato root types during storage root formation. Plant Growth Regul 75, 155–164 (2015). https://doi.org/10.1007/s10725-014-9940-x

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  • DOI: https://doi.org/10.1007/s10725-014-9940-x

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