Abstract
Plant-specific NAC transcription factors comprise a large family with diverse roles in plant development and stress regulation. In this study, 73 NAC genes from cotton (Gossypium hirsutum) EST database were identified by bioinformatic approach. Analysis of conserved amino acid residues and phylogeny reconstruction using the NAC conserved domain suggested that the Arabidopsis classification into four major groups is applicable to cotton NAC family. Among them, seven NAC genes, named as GhNAC7–GhNAC13, were characterized to encode NAC proteins that share high similarity with those plant abiotic stress-related NACs. Quantitative RT-PCR analysis indicated that the seven GhNAC genes were preferentially expressed in roots, and regulated in cotton plants under cold, abscisic acid, drought and/or high salt conditions. Our results in this comprehensive study of cotton NAC gene family provide valuable information for further exploring the roles of the NAC genes in cotton development and in response to abiotic stress.
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Acknowledgments
This work was supported by the National Natural Sciences Foundation of China (Grant No. 30900073), the project from the Ministry of Agriculture of China for transgenic research (Grant No. 2011ZX08009-003), and the Scientific Research Foundation of Hubei Province (Grant No. 2011CDA140).
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Geng-Qing Huang and Wen Li contributed equally to this work.
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Huang, GQ., Li, W., Zhou, W. et al. Seven cotton genes encoding putative NAC domain proteins are preferentially expressed in roots and in responses to abiotic stress during root development. Plant Growth Regul 71, 101–112 (2013). https://doi.org/10.1007/s10725-013-9811-x
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DOI: https://doi.org/10.1007/s10725-013-9811-x