Saponin production is not qualitatively changed upon callus regeneration in the medicinal shrub Maesa perlarius
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Maesa perlarius is a medicinal plant that produces maesabalides, which possess selective and strong anti-leishmania activity. In this study, M. perlarius plants were regenerated from leaf-derived calli. Shoots were induced in Murashige and Skoog medium in the presence of thidiazuron in combination with α-naphthalene acetic acid. In contrast to seed-derived plants, callus-derived regenerants were tetraploid showing typical characteristics of higher ploidy phenotypes. We assessed the impact of indirect plant regeneration and associated increase in ploidy on the production of saponin by means of LC–MS analysis. Tetraploid M. perlarius produce a saponin profile, which was not significantly different from seed grown plants. Based on this study, we concluded that saponin production in M. perlarius is not qualitatively changed by a genome-doubling event.
KeywordsCallus regeneration Maesa perlarius Medicinal secondary metabolites Saponin Polyploidy
We thank the BOF (special research fund No. 01WI0111, Ghent University) for the A.F. pre-doctoral fellowship. This research is supported by a research grant G.0014.08 offered by the Flemish Funding Agency for Scientific Research (FWO).
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