Abstract
Recent findings have suggested that H2O2 is an important signaling molecule for regulating plant responses to abiotic stress. H2O2 plays a critical role in NaCl stress. Heme oxygenase (HO) is known to play a protective role against oxidative stress. In this study, we examined the possible involvement of H2O2 in regulating NaCl-promoted HO activity in rice roots. Treatment with NaCl increased HO activity and H2O2 content in rice roots. As well, NaCl could induce OsHO1 mRNA expression. NaCl (150 mM) and NaNO3 (150 mM) were equally effective in inducing HO activity. However, mannitol at the concentration (276 mM) iso-osmotic with 150 mM NaCl had no effect on HO activity. NaCl-promoted HO activity and OsHO1 expression in rice roots was reduced by NADPH oxidase inhibitors i.e. dipehnyleneiodonium and imidazole. Moreover, exogenous application of H2O2 enhanced the activity of HO and the mRNA level of OsHO1. Our data suggest that H2O2 production plays a positive role in NaCl- induced HO activity by enhancing its mRNA level in rice roots.
Similar content being viewed by others
Abbreviations
- CAT:
-
Catalase
- DPI:
-
Diphenyleneiodonium
- DW:
-
Dry weight
- HO:
-
Heme oxygenase
- IMD:
-
Imidazole
- Man:
-
Mannitol
- ROS:
-
Reactive oxygen species
- SOD:
-
Superoxide dismutase
References
Balestrsse KB, Noriega GO, Battle A, Tomaro ML (2005) Involvement of heme oxygenase as antioxidant defense in soybean nodules. Free Radic Res 39:145–151
Balestrsse KB, Noriega GO, Battle A, Tomaro ML (2006) Heme oxygenase activity and oxidative stress signaling in soybean leaves. Plant Sci 170:339–346
Chen X-Y, Ding X, Xu S, Wang R, Xuan W, Cao Z-Y, Chen J, Wu H-H, Ye M-B, Shen W-B (2009) Endogenous hydrogen peroxide plays a positive role in the upregulation of heme oxygenase and acclimation to oxidative stress in wheat seedling leaves. J Integr Plant Biol 51:951–960
Dat J, Vandenabeele S, Vranova E, Van Montagu M, Inze D, Van Breusegem F (2000) Dual action of the active oxygen species during plant stress responses. Cell Mol Life Sci 57:779–795
Davis SJ, Kurepa J, Vierstra RD (1999) The Arabidopsis thaliana HY1 locus, requird for phytochrome-chromophore biosynthesis, encodes a protein related to heme oxygenases. Proc Natl Acad Sci USA 96:6541–6546
Han B, Xu S, Xie Y-J, Huang J-J, Wang L-J, Yang Z, Zhang C-H, Sun Y, Shen WB, Xie G-S (2012) ZmHO-1, a maize haem oxygenase-1gene, plays a role in determining lateral root development. Plant Sci 184:63–74
Hong C-Y, Chao Y-Y, Yang M-Y, Cheng S-Y, Cho S-C, Kao CH (2009) NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid. Plant Soil 320:103–115
Izawa T, Oikawa T, Tokutomi S, Okuno K, Shimamoto K (2000) Phytochromes confer the photoperiodic control of flowering in rice (a short-day plant). Plant J 22:391–399
Jana S, Choudhuri MA (1981) Glycolate metabolism of three submerged aquatic angiosperms: effect of heavy metals. Aquat Bot 11:67–77
Lee DH, Kim YS, Lee CB (2001) The inductive responses of the antioxidant enzymes by salt stress in the rice (Oryza sativa L.). J Plant Physiol 158:734–774
Li MY, Cao ZY, Shen WB, Cui J (2011) Molecular cloning and expression of a cucumber (Cucumis sativus L.) heme oxygenase 1 gene, CsHO1, which is involved in adventitious root formation. Gene 486:47–55
Lin CC, Kao CH (1999) NaCl induced changes in ionically bound peroxidase activity in roots of rice seedlings. Plant Soil 216:147–153
Lin CC, Kao CH (2001) Cell wall peroxidase activity, hydrogen peroxide level and NaCl-inhibited root growth of rice seedlings. Plant Soil 230:135–143
Linley PJ, Landsberger M, Kohchi T, Cooper JB, Terry MJ (2006) The molecular basis of heme oxygenase deficiency in the pcd1 mutant of pea. FEBS J 273:2594–2606
Muramoto T, Kohchi T, Yokota A, Hwang I, Goodman HM (1999) The Arabidopsis photmorphogenic mutant hy1 is deficient in phytocherome chromophore biosynthesis as a result of a mutation in a plastid heme oxygenase. Plant Cell Physiol 11:335–348
Noriega GO, Balestrasse KB, Battle A, Tomaro ML (2004) Heme oxygenase exerts a protective role against oxidative stress in soybean leaves. Biochem Biophys Res Commun 323:1003–1008
Orozo-Cardenas ML, Narvez-Vaaquez J, Ryan CA (2001) Hydrogen peroxide acts as a second messenger for the induction of defense genes in tomato plants in response to wounding, systemin, and methyl jasmonate. Plant Cell 13:179–191
Shen Z, Jiang M, Li H, Che LL, Yang ZM (2011) Expression of a Brassica napus heme oxygenase confers plant tolerance to mercury toxicity. Plant, Cell Environ 34:752–763
Synder XH, Baranano D (2001) Heme oxygenase: a font of multiple messengers. Neuropsychopharmacology 25:294–298
Terry MJ, Kendrick RE (1996) The aurea and yellow-green-2 mutants of tomato are deficient in phytochrome chromophore synthesis. J Biol Chem 271:21681–21686
Tsai Y-C, Hong C-Y, Liu L-F, Kao CH (2004) Relative importance of Na+ and Cl− in NaCl-induced antioxidant systems in roots of rice seedlings. Physiol Plant 122:86–94
Tsai Y-C, Hong CY, Liu L-F, Kao CH (2005) Expression of ascorbate peroxidase and glutathione reductase in roots of rice seedlings in response to NaCl to H2O2. J Plant Physiol 162:291–299
Xuan W, Zhu F-Y, Xu S, Huang B-K, Ling T-F, Zi J-Y, Ye M-B, Shen W-B (2008) The heme oxygenase/carbon monoxide system is involved in the auxin-induced cucumber adventitious process. Plant Physiol 148:881–893
Yannarelli GG, Noriega GO, Battle A, Tomaro ML (2006) Heme oxygenase up-regulation in ultraviolet-B irradiated soybean plants involves reactive species. Planta 224:1154–1162
Zilli CG, Balestrasse KB, Yannarelli GG, Polizio AH, Santa-Cruz EM, Tomaro ML (2008) Heme oxygenase up regulation under salt stress protects nitrogen metabolism in nodules of soybean plants. Environ Exp Bot 64:83–89
Acknowledgments
This work was supported by a research Grant from the National Science Council of the Republic of China (NSC 100-2313-B002-002).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Wei, MY., Chao, YY. & Kao, C.H. NaCl-induced heme oxygenase in roots of rice seedlings is mediated through hydrogen peroxide. Plant Growth Regul 69, 209–214 (2013). https://doi.org/10.1007/s10725-012-9762-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10725-012-9762-7