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UV-induced differential gene expression in rice cultivars analyzed by SSH

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Abstract

The differences in gene expression between two rice genotypes—the ultraviolet-B (UV-B)-resistant cultivar Lemont and the UV-B-sensitive cultivar Dular—in response to irradiation with UV-B were studied. Suppression subtractive hybridization and real-time quantitative reverse transcriptase polymerase chain reaction were used in the analysis. The results showed that enhanced UV-B radiation could induce differential expression of the genes related to defense response. The expression of genes encoding HECT domain-containing protein, thylakoid-bound ascorbate peroxidase, receptor-like protein kinase, Rad6 (ubiquitin carrier protein), phenylalanine ammonia-lyase, 4-coumarate: CoA ligase and chalcone synthase was up-regulated to different extents in the two rice cultivars: The cultivar Lemont exhibited a higher increase in the gene expression compared with the cultivar Dular. A large difference was found in the expression pattern of the universal stress protein (USP): showing down-regulation in Dular but up-regulation in Lemont. Hence exposure to UV-B radiation has a significant effect on gene expression in rice. In response to UV exposure, a molecular response is initially induced in different UV-B-resistant rice cultivars. This response may activate secondary metabolic pathways, in turn increase the synthesis of UV-protective substances (flavonoids) to prevent UV-B damage and induce the transcription of the relevant mRNAs for repairing the damaged DNA.

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Abbreviations

SSH:

Suppression subtractive hybridization

qRT-PCR:

Real-time quantitative reverse transcriptase polymerase chain reaction

UV-B:

Ultraviolet-B

PAL:

Phenylalanine ammonia-lyase

PKD:

Protein kinase domain containing protein

HECT:

HECT domain containing protein

USP:

Universal stress protein

tAPX:

Thylakoid-bound ascorbate peroxidase

4CL:

4-Coumarate: CoA ligase

CHS:

Chalcone synthase

RLK:

Receptor-like protein kinase

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Acknowledgments

The project was financially supported by National Natural Science Foundation of China (No.30571103, 30971737) and National Research Foundation for the Doctoral Program of Higher Education of China (No.20050389004).

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Authors and Affiliations

  1. Agroecological Institute/School of Life Sciences, Fujian Agriculture and Forestry University (FAFU), 350002, Fuzhou, People’s Republic of China

    Chang-Xun Fang, Xing-Chun Wu, Hai-Li Zhang, Xiong Jun, Wen-Xiang Wu & Wen-Xiong Lin

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  1. Chang-Xun Fang
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Correspondence to Wen-Xiong Lin.

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Fang, CX., Wu, XC., Zhang, HL. et al. UV-induced differential gene expression in rice cultivars analyzed by SSH. Plant Growth Regul 59, 245–253 (2009). https://doi.org/10.1007/s10725-009-9411-y

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