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Spectral composition of irradiation regulates the cell growth and flavonoids biosynthesis in callus cultures of Saussurea medusa Maxim

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Abstract

Cell growth, flavonoids biosynthesis and L-phenylalanine ammonia-lyase (PAL) activity were studied in callus cultures of Saussurea medusa Maxim. under different types of spectral radiance. After 21 days, red light significantly improved the callus growth, but inhibited the biosynthesis of flavonoids in callus cultures. However, blue light was found to enhance the biosynthesis of flavonoids, although callus growth under this spectrum was comparable with that under white and other coloured spectra, such as green and yellow. The accumulation of flavonoids in callus cultures was related to the PAL activity, which was found to be stimulated by the spectral composition of irradiation.

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Abbreviations

BA:

N6-benzyladenine

MS:

Murashige and Skoog (1962)

NAA:

α-Naphthaleneacetic acid

PAL:

L-phenylalanine ammonia-lyase

PFD:

Photosynthetic fluence density

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Authors and Affiliations

  1. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100080, P. R. China

    Bin Guo, Qiong Yan & Chun-Zhao Liu

  2. College of Food Science & Nutritional Engineering, China Agricultural University, Beijing, 100093, P. R. China

    Yong-Gang Liu

  3. Arkansas Biosciences Institute, Arkansas State University, Jonesboro, AR, 72401, USA

    Chun-Zhao Liu

  4. Graduate School of the Chinese Academy of Sciences, Beijing, 100049, P. R. China

    Bin Guo

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  1. Bin Guo
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  2. Yong-Gang Liu
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  3. Qiong Yan
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  4. Chun-Zhao Liu
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Correspondence to Chun-Zhao Liu.

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Guo, B., Liu, YG., Yan, Q. et al. Spectral composition of irradiation regulates the cell growth and flavonoids biosynthesis in callus cultures of Saussurea medusa Maxim. Plant Growth Regul 52, 259–263 (2007). https://doi.org/10.1007/s10725-007-9192-0

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  • DOI: https://doi.org/10.1007/s10725-007-9192-0

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