Abstract
Rehmannia glutinosa seedlings were pretreated with choline chloride (CC) in concentrations of 0, 0.7, 2.1 and 3.5 mM, and then subjected to drought and rewatering treatment to study the effects of CC on the generation of reactive oxygen species (O −2 , H2O2), lipid peroxidation, proline accumulation, water status and photosynthesis. The results showed that pretreatment with CC alleviated the inhibition of SOD and APX activity caused by drought stress, and therefore, the rate of O −2 production and H2O2 concentration were reduced and lipid peroxidation decreased in pretreated plants. CC pretreatment also accelerated accumulation of proline, maintained higher Ψw and RWC, deferred leaf water loss during drought stress and retarded the drop in proline concentration after rewatering. Consequently, drought-induced decreases in Fm/F0, Fv/Fm, ΦPS2, qP, and A and increase in qNP were inhibited and the recovery of photosynthesis after rewatering was quicker in pretreated plants. Although differences in Fv/Fm, ΦPS2 and qP between treatments were not significant, there was a general trend that the effects of CC increased with the rise of its concentrations. The data suggested that 2.1 mM of CC be suitable for alleviating lipid peroxidation, promoting proline accumulation, retarding leaf water loss and improving photosynthesis of R. glutinosa seedlings under drought stress.
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References
Asada K (1994) Production and action of active oxygen species in photosynthetic tissues. In: Foyer C, Mullineaux PM (eds) Causes of photooxidative stress and amelioration of defense systems in plants. CRC Press, Boca Raton, London, pp 77–100
Bates LS, Waldren SP, Teare ID (1973) Rapid determination of free proline for water-stressed studies. Plant Soil 39:205–207
Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and assay for acrylamide gels. Ann Biochem 44:267–278
Bowler C, Van Montagu M, Inze D (1992) Superoxide dismutase and stress tolerance. Annu Rev Plant Physiol Plant Mol Biol 43:83–116
Bray E (1993) Molecular responses to water deficit. Plant Physiol 103:1035–1040
Diaz P, Borsani O, Marquez A, Monza J (2005) Osmotically induced proline accumulation in Lotus corniculatus leaves is affected by light and nitrogen source. Plant Growth Regul 46:223–232
Elstner EF, Heupel A (1976) Inhibition of nitrite formation from hydroxylammonium chloride: a simple assay for superoxide dismutase. Anal Biochem 70:616–620
Genty B, Briantais JM, Baker NR (1989) The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochimica et Biophysica Acta 990:87–92
Jana S, Chaudhuri A (1981) Glycolate metabolism of three submerged aquatic angiosperms during aging. Aquat Bot 12:345–354
Kreslavski VD, Balakhnina TI, Khristin MS, Bukhov NG (2001) Pre-treatment of bean seedlings with choline compounds increases the resistance of photosynthetic apparatus to UV-B radiation and elevated temperature. Photosynthetica 39(3):363–368
Kreslavski V, Kobzar E, Ivanova E, Kuznetsov E (2005) Effects of short-term red radiation and choline compounds on cytokinin content, chlorophyll accumulation and photomorphogenesis. Plant Growth Regul 47:9–15
Larkindale J, Knight MR (2002) Protection against heat stress-induced oxidative damage in Arabidopsis involves calcium, abscisic acid, ethylene, and salicylic acid. Plant Physiol 128:682–693
Li L, Van Staden J, Jager AK (1998) Effects of plant growth regulators on the antioxidant system in seedlings of two maize cultivars subjected to water stress. Plant Growth Regul 25:81–87
Maggio A, Miyazaki S, Veronese P, Fujita T, Ibeas JI, Damsz B, Narasimhan ML, Hasegawa PM, Joly RJ, Bressan RA (2002) Does proline accumulation play an active role in stress-induced growth reduction? Plant J 31:699–712
Nakano Y, Asada K (1981) Hydrogen peroxide scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880
Novitskaya GV, Kocheshkova TK, Feofilaktova TV, Novitskii Yu I (2004) Effects of choline chloride on the lipid content and composition in the leaves of principal magnetically-oriented radish types. Russian J Plant Physiol 51(3):361–371
Schreiber U, Schliwa U, Bilger W (1986) Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. Photosynth Res 10:51–62
Sharma P, Dubey RS (2005) Drought induces oxidative stress and enhances the activities of antioxidant enzymes in growing rice seedlings. Plant Growth Regul 46:209–221
Singh B, Usha K (2003) Salicylic acid induced physiological and biochemical changes in wheat seedlings under water stress. Plant Growth Regul 39:137–141
Smirnoff N (1993) The role of active oxygen in the response of plants to water deficit and desiccation. New Phytol 125:27–58
Xing H, Tan L, An L, Zhao Z, Wang S, Zhang C (2004) Evidence for the involvement of nitric oxide and reactive oxygen species in osmotic stress tolerance of wheat seedlings: inverse correlation between leaf abscisic acid accumulation leaf water loss. Plant Growth Regul 42:61–68
Zhao HJ, Zou Q (2002) Protective effects of exogenous antioxidants and phenolic compounds on photosynthesis of wheat leaves under high irradiance and oxidative stress. Photosynthetica 40(4):523–527
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This research was financially supported by Science and Technology Fund of Henan Province, P. R. China (0524420042).
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Zhao, H., Tan, J. & Qi, C. Photosynthesis of Rehmannia glutinosa subjected to drought stress is enhanced by choline chloride through alleviating lipid peroxidation and increasing proline accumulation . Plant Growth Regul 51, 255–262 (2007). https://doi.org/10.1007/s10725-007-9167-1
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DOI: https://doi.org/10.1007/s10725-007-9167-1