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Use of AFLPs to detect hybrids that confuse species boundaries between the lesser known African taxa in Solanum Merolloids section (Solanaceae)

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Abstract

This study was designed to assess whether or not existed interspecific hybrids between the two lesser known African taxa in Solanum Merolloids section Solanum; S. tardremotum and S. florulentum that confused their taxonomy. Hybridization is one of the factors that blur boundary between closely related species. It is an indication that there is semi-permeable boundary through which genes are flowing across. In the African species of section Solanum it has been hypothesized that interspecific hybridization is one of the factors complicating the delimitation of species though this claim has never been examined systematically. Solanum tarderemotum and S. florulentum were all described by Bitter from Tanzania. Through history the two taxa have been considered separate species, different species mixed with hybrids and recently the boundary of S. tarderemotum has been expanded to include S. florulentum s. str. and materials thought to be hybrids. DNA was isolated from 63 individuals belonging to 22 accessions and two amplified fragment length polymorphism (AFLP) primer combinations; EcoRI + AAC/MseI + CAC and EcoRI + ACC/MseI + CAT used. Clustering used PAST software. Five clusters were generated, S. tarderemotum s. str. and S. florulentum s. str., fell in different clusters. The two taxa shared only 41% of their bands which was less that what was shared between them and their supposed hybrids in two of the three remaining clusters. Some individuals from certain accessions segregated in different clusters. Intermediary and segregation indicates exists hybrids in midst of good species S. tarderemotum and S. florulentum and this could be the reason of the confused species delimitation.

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Dataset generated and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

My sincere thanks to ENVIRON Project conducted between University of Dar es Salaam and Radboud University Nijmegen for supporting the study. Richard Feron a Techncian at Experiemntal Botany Laboratory asisited with AFLP analysis.

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The author Declare that data for this publication was generated during a PhD study that was supported by a project conducted between the University of Dar es Salaam and Radboud University Nijmegen.

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  1. Department of Crop Sciences and Beekeeping Technology, University of Dar es Salaam, Box 35314, Dar es Salaam, Tanzania

    Mkabwa L. K. Manoko

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I am the sole author of the publication. A person who assited with PCR  is thanked.

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Manoko, M.L.K. Use of AFLPs to detect hybrids that confuse species boundaries between the lesser known African taxa in Solanum Merolloids section (Solanaceae). Genet Resour Crop Evol 71, 1113–1123 (2024). https://doi.org/10.1007/s10722-023-01680-y

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