Abstract
The locally cultivated creole varieties of Phaseolus lunatus are adapted to specific climatic and environmental conditions. Family farmers and local communities preserve and multiply their seeds over generations, promoting genetic diversity, food and nutritional security, and agricultural sustainability. This species has great geno-phenotypic diversity, which can be harnessed in breeding programs if accurately characterized. We evaluated the phenotypic variations of P. lunatus seeds from 13 varieties in three states (Sergipe, Bahia, and Alagoas) using image analysis. We estimated the weight of 100 seeds using a precision analytical balance and obtained morphometric measurements, including area, maximum diameter, and minimum diameter, using Groundeye (TBit®) imaging equipment and software. We also recorded dominant color and RGB color system descriptors. The morphometric variables underwent variance analysis using the F-test, and the means were clustered using the Scott-Knott test at 5% significance level. The data underwent Pearson Correlation Analysis (t-Student at 5%), were grouped based on dissimilarity using the UPGMA method, and were represented in a dendrogram. We also performed Principal Component Analysis on the evaluated characteristics. The dominant color of the seeds was predominantly orange in nine varieties. Morphometry showed a positive and significant association. The dendrogram revealed two homogeneous and distinct groups, and the first two principal components accounted for 86.80% of the genotypic variation. Therefore, high-resolution images for phenotypic characterization of creole lima bean seeds are a promising non-destructive tool for selection purposes.
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Data availability
The datasets produced and/or analyzed during the current study are available upon reasonable request from the corresponding author.
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Acknowledgements
We would like to acknowledge the financial support provided by the Coordination for the Improvement of Higher Education Personnel – Brazil (CAPES- 001). We also thank the Federal University of Sergipe and the Research Group on Conservation, Breeding, and Management of Genetic Resources (GENAPLANT) for their valuable contribution to this study.
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Coordination for the Improvement of Higher Education Personnel – Brazil (CAPES-001).
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SJD conducted the research and wrote the manuscript, MFOT contributed to data acquisition. RSM and PFV supervised the research, revised the manuscript, and contributed to writing, translating, and revising the text. All authors contributed to the article and approved the submitted version.
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de Jesus Dantas, S., Torres, M.F.O., Silva-Mann, R. et al. Phaseolus lunatus L.: pulse seeds phenotype image analysis. Genet Resour Crop Evol 70, 2555–2565 (2023). https://doi.org/10.1007/s10722-023-01583-y
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DOI: https://doi.org/10.1007/s10722-023-01583-y