Abstract
Tunisia, other countries in northwestern Africa, and the Iberian Peninsula, represent a center of diversity of Daucus. The genus traditionally has included about 20–25 species worldwide, but a recent molecular study redefined and expanded Daucus to include representatives from nine other genera. By this classification, Daucus now contains about 40 species, with some of them having winged fruits in addition to its traditionally recognized spiny fruits. The taxonomy of Daucus in Tunisia has recently been studied with morphological data, concluding that D. carota subsp. capillifolius is a subspecies that co-occurs and crosses with subsp. carota. The present study extends these findings with additional data from Genotyping-by-Sequencing (GBS) from 33 Tunisian accessions of D. carota (93 individuals with two to three replicates per accession), including the first collections of subsp. gummifer from Tunisia. Placed in the context of additional GBS data, D. carota subsp. gummifer has separate origins from other collections of subsp. carota in Tunisia and/or immediately adjacent areas to the north in Italy or its surrounding islands. Our results add support to the utility of large SNP datasets for species-level phylogenetic studies in Daucus.
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Acknowledgements
The authors thank Doctors Zeineb Grabi from the National Institute of Agronomy of Tunisia, Houda Kourda from the National Gene Bank of Tunisia, Slim Rouz from the High School of Agriculture of Mogran, Tunisia, and Mohamed Neffati from the Institute of Arid Regions-Medenine, Tunisia for their contribution in collecting Tunisian accessions and the University of Wisconsin Biotechnology Center DNA Sequencing Facility for providing GBS facilities and services.
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This work was supported by a grant from the Fulbright Visiting Scholar Program.
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Supplementary Table
1: The 183 accessions of Daucus L. characterized in this study, improvement status, specific and generalized localities, and geographic coordinates (DOC 312 kb)
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Mezghani, N., Ruess, H., Tarchoun, N. et al. Genotyping-by-sequencing reveals the origin of the Tunisian relatives of cultivated carrot (Daucus carota). Genet Resour Crop Evol 65, 1359–1368 (2018). https://doi.org/10.1007/s10722-018-0619-4
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DOI: https://doi.org/10.1007/s10722-018-0619-4