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Genetic diversity analysis in Phaseolus vulgaris L. using morphological traits

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Abstract

Common bean (Phaseolus vulgaris) is an important export crop in Kyrgyzstan since the end of the twentieth century. Genetic diversity analysis of common bean populations is useful for breeding programs, as it helps to select genetic material to be used for further crossing. Twenty-seven common bean accessions were analyzed using 13 qualitative morphological traits. In some cases, obtained morphological data were combined with previously published results based on microsatellite markers. The similarity matrices generated from the molecular and morphological data were significantly correlated (r = 0.49, P < 0.01). Cluster analyses based on Dice’s similarity coefficient were constructed based on morphological data and the combined data set of morphology and microsatellite, and both grouped the 27 accessions according to their origin: 15 belonged to the Andean and 12 to the Mesoamerican gene pool. On average, the Andean accessions were less diverse than the Mesoamerican accessions. The average diversity based on the Shannon diversity index for the 13 qualitative morphological traits was 0.05. Overall, this study revealed that qualitative morphological markers are efficient in assigning modern cultivars to their gene pools of origin.

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Acknowledgments

This research was undertaken as part of the PhD study of the first author. We are thankful for the funding provided by the Swedish International Development Agency (Sida) and and Einar and Inga Nilsson fund. We thank Michigan State University (East Lansing) and United States Department of Agriculture (Pullman) for providing the common bean germplasm used in this study. Special thanks to Dr. Elie Marx (INRA, France) for guidance regarding phenotyping of accessions. We are grateful to Anna Zborowska (SLU) for her laboratory assistance.

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Authors and Affiliations

  1. Department of Plant Breeding, Swedish University of Agricultural Sciences, Box 101, 23053, Alnarp, Sweden

    Sergey Hegay, Mulatu Geleta, Tomas Bryngelsson, Larisa Garkava-Gustavsson, Helena Persson Hovmalm & Rodomiro Ortiz

  2. Department of Agronomy, Kyrgyz National Agrarian University, Mederova 68, 720005, Bishkek, Kyrgyz Republic

    Sergey Hegay & Abdybek Asanaliev

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  1. Sergey Hegay
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Correspondence to Sergey Hegay.

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Hegay, S., Geleta, M., Bryngelsson, T. et al. Genetic diversity analysis in Phaseolus vulgaris L. using morphological traits. Genet Resour Crop Evol 61, 555–566 (2014). https://doi.org/10.1007/s10722-013-0056-3

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