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Analysis of genetic diversity and population structure of 135 dill (Anethum graveolens L.) accessions using RAPD markers

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Abstract

Dill (Anethum graveolens L.) leaf, seed and their essential oil are rich source of antioxidants. The plant is native in Southwest Asia and is cultivated in Europe, India and the United States. This study evaluated the genetic diversity structure of 135 accessions of A. graveolens from different continents, based on random amplified polymorphic DNA (RAPD) markers. The selected 10 RAPD primers generated a total of 142 highly reproducible bands, among which 89 were polymorphic. Percentage of polymorphism varied from 41.17 % (OPB20) to 92.85 % (OPB15) with an average of 77.74 %. A relatively high genetic diversity was detected among all the accessions with the Nei’s genetic diversity (H) values ranged from 0.346 (OPB07) to 0.444 (OPB18) with a mean of 0.401. When estimated for Shannon’s information index (I), it has ranged from 0.530 (OPB12) to 0.652 (OPB18), the mean was observed as 0.581. The respective values of H and I were found to be the highest value for primer OPB18. Cluster analysis of RAPD data using UPGMA algorithm based on Nei’s genetic similarity matrix placed the 135 accessions into two main clusters. Although a number of groups can be identified, the clusters show little to no association with the geographic origin of the material. The implication of the results of this study in developing a strategy for the conservation and breeding of dill germplasm are discussed.

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References

  • Chaubey MK (2008) Fumigant toxicity of essential oils from some common spices against pulse beetle, Callosobruchus chinensis (Coleoptera: Bruchidae). J Oleo Sci 57:171–179

    Article  PubMed  CAS  Google Scholar 

  • Dangi RS, Lagu MD, Choudhary LB, Ranjekar PK, Gupta VS (2004) Assessment of genetic diversity in Trigonella foenum-graecum and Trigonella caerulea using ISSR and RAPD markers. BMC Plant Biol 4:13. doi:10.1186/1471-2229-4-13

    Article  PubMed  Google Scholar 

  • Delaquis PJ, Stanich K, Girard B, Mazza G (2002) Antimicrobial activity of individual and mixed fractions of dill, cilantro, coriander and eucalyptus essential oils. Int J Food Microbiol 74:101–109. doi:10.1016/j.foodchem.2005.01.051

    Article  PubMed  CAS  Google Scholar 

  • Dellaporta S, Wood J, Hicks JB (1983) A plant DNA minipreparation: version l. Plant Mol Biol Rep 1:19–21

    Article  CAS  Google Scholar 

  • Guo HB, Li SM, Peng J, Ke WD (2007) Genetic diversity of Nelumbo accessions revealed by RAPD. Genet Resour Crop Evol 54:741–748. doi:10.1007/s10722-006-0025-1

    Article  Google Scholar 

  • Heywood VH (1971) Biology and chemistry of the Umbelliferae. Academic Press, London

    Google Scholar 

  • Jana S, Shekhawat GS (2012) In vitro regeneration of Anethum graveolens, antioxidative enzymes during organogenesis and RAPD analysis for clonal fidelity. Biol Plant 56(1):9–14. doi:10.1007/s10535-011-0223-3

    Article  CAS  Google Scholar 

  • Just T. (2008) “Dill” Encyclopedia Americana. Grolier online, http://ea.grolier.com/cgi-bin/article?assetid=0127540-00. 30 June 2008

  • Khalaf AF (2004) Toxicological efficacy of some indigenous dill compounds against the flesh fly, Parasarcophaga dux Thomson. J Egypt Soc Parasitol 34:227–237

    PubMed  Google Scholar 

  • Kmiecik W, Gebczynski P, Jaworska G (2001) Effect of the cultivar, usable part and growing period on the content of selected antioxidative components in dill (Anethum graveolens L.). Acta Agraria et Silvestria Agraria 39:35–48

    Google Scholar 

  • Lanky PS, Schilcher H, Phillipson JD, Loew D (1993) Plants that lower cholesterol. Acta Hortic 332:131–136

    Google Scholar 

  • Lazutka JR, Mierauskiene J, Slapsyte G, Dedonyte V (2001) Genotoxicity of dill (Anethum graveolens L.), peppermint (Mentha piperita L.) and pine (Pinus sylvestris L.) essential oils in human lymphocytes and Drosophila melanogaster. Food Chem Toxicol 39:485–492. doi:10.1016/S0278-6915(00)00157-5

    Article  PubMed  CAS  Google Scholar 

  • Loveless MD, Hamrick JL (1984) Ecological determinants of genetic structure in plant populations. Ann Rev Ecol Syst 15:65–69. doi:10.1146/annurev.es.15.110184.000433

    Article  Google Scholar 

  • Ma XH, Qin RL, Xing WB (1984) Chromosome observations of some medical plants in Xinjiang. Acta Phytotax Sin 22:243–249

    Google Scholar 

  • Mahran GH, Kadry HA, Isaac ZG, Thabet CK, Al-Azizi MM, El-Olemy MM (1992) Investigation of diureic drug plats. 1. Phytochemical investigation and pharmacological evaluation of Anethum graveolens L., Apium graveolens L., Daucus carota L. and Eruca sativa Mill. Phytother Res 5:169–172. doi:10.1002/ptr.2650050406

    Article  Google Scholar 

  • Martins SR, Vences FJ, Saenz de Miera LE, Barrosa MR, Carnide V (2006) RAPD analysis of genetic diversity among and within Portuguese landraces of common white bean (Phaseolus vulgaris L.). Sci Hort 108:133–142

    Article  CAS  Google Scholar 

  • Mazyad SA, El-Serougi AO, Morsy TA (1999) The efficacy of the volatile oils of three plants for controlling Lucilia sericata. J Egypt Soc Parasitol 29:91–100

    PubMed  CAS  Google Scholar 

  • Mohammad Al-Ismail K, Aburjai T (2004) Antioxidant activity of water and alcohol extracts of chamomile flowers, anise seeds and dill seeds. J Sci Food Agric 84:173–178. doi:10.1002/ptr.2650050406

    Article  Google Scholar 

  • Monsefi M, Ghasemi M, Bahaoddini A (2006) The effects of Anethum graveolens L. on female reproductive system. Phytother Res 20:865–868. doi:10.1002/ptr.1959

    Article  PubMed  CAS  Google Scholar 

  • Naseri MKG, Heidari A (2007) Antispasmodic effect of Anethum graveolens fruit extract on rat ileum. Int J Pharmacol 3:260–264. doi:ijp.2007.260264

    Article  Google Scholar 

  • Nybom H (2004) Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants. Mol Ecol 13(5):1143–1155. doi:10.1111/j.1365-294X.2004.02141.x

    Article  PubMed  CAS  Google Scholar 

  • Nybom H, Bartish V (2000) Effects of life history traits and sampling strategies on genetic diversities estimates obtained with RAPD markers in plants. Perspect Plant Ecol Evol Syst 3:93–114. doi:10.1078/1433-8319-00006

    Article  Google Scholar 

  • O’Neill R, Snowdon RJ, Kohler W (2003) Population genetics aspects of biodiversity. Prog Bot 64:115–137

    Article  Google Scholar 

  • Paterson AH, Damon S, Hewitt JD, Zamir D, Rabinowitch HD (1991) Mendelian factors underlying quantitative traits in tomato: comparison across species, generations, environments. Genetics 127:181–197

    PubMed  CAS  Google Scholar 

  • Rossetto M, Weaver PK, Dixon KW (1995) Use of RAPD analysis in devising conservation strategies for the rare and endangered Grevillea scapigera (Proteaceae). Mol Ecol 4:321–329. doi:10.1111/j.1365-294X.1995.tb00225.x

    Article  PubMed  CAS  Google Scholar 

  • Seo SM, Kim J, Lee SG, Shin CH, Shin SC, Park IK (2009) Fumigant antitermitic activity of plant essential oils and components from Ajowan (Trachyspermum ammi), Allspice (Pimenta dioica), Caraway (Carum carvi), Dill (Anethum graveolens), Geranium (Pelargonium graveolens), and Litsea (Litsea cubeba) oils against Japanese termite (Reticulitermes speratus Kolbe). J Agric Food Chem 57:6596–6602. doi:10.1021/jf9015416

    Article  PubMed  CAS  Google Scholar 

  • Singh G, Maurya S, Lampasona MP, de Catalan C (2005) Chemical constituents, antimicrobial investigations, and antioxidative potentials of Anethum graveolens L. essential oil and acetone extract: Part 52. J Food Sci 70(4):M208–M215. doi:10.1111/j.1365-2621.2005.tb07190.x

    Article  CAS  Google Scholar 

  • Snell R, Aarssen LW (2005) Life history traits in selfing versus outcrossing annuals: exploring the ‘time-limitation’ hypothesis for the fitness benefit of self-pollination. BMC Ecol 5:2. doi:10.1186/1472-6785-5-2

    Article  PubMed  Google Scholar 

  • Solouki M, Mehdikhani H, Zeinali H, Emamjomeh AA (2008) Study of genetic diversity in Chamomile (Matricaria chamomilla) based on morphological and molecular markers. Sci Hortic 117:281–287. doi:10.1016/j.scienta.2008.03.029

    Article  CAS  Google Scholar 

  • Solouki M, Hoseini SB, Siahsar BA, Tavassoli A (2012) Genetic diversity in dill (Anethum graveolens L.) populations on the basis of morphological traits and molecular markers. Afr J Biotechnol 11(15):3649–3655. doi:10.5897/AJB11.2169

    Article  CAS  Google Scholar 

  • Tucker AO (2008) “Dill” Grolier multimedia encyclopedia. Grolier online. http://gme.grolier.com/cgi-bin/article?assetid=0085590-0. 30 June 2008

  • Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535. doi:10.1093/nar/18.22.6531

    Article  PubMed  CAS  Google Scholar 

  • Wu HF, Li ZZ, Huang H (2006) Genetic differentiation among natural populations of Gastrodia elata (Orchidaceae)in Hubei and germplasm assessment of the cultivated populations. Biodivers Sci 14:315–326

    Article  Google Scholar 

  • Yan ZF, Zhang BG, Zhang Z, Yu JL (2006) Genetic diversity in wild populations of Phellodendron amurense a rare and endangered medicinal plant, detected by AFLP. Biodivers Sci 14:488–497

    Article  CAS  Google Scholar 

  • Yang Y, Huang CY, Peng SS, Li J (1996) Carotenoid analysis of several dark green leafy vegetables associated with a lower risk of cancers. Biomed Environ Sci 9:386–392

    PubMed  CAS  Google Scholar 

  • Yazdanparast R, Alavi M (2001) Antihyperlipidaemic and antihypercholesterolaemic effects of Anethum graveolens leaves after the removal of furocoumarins. Cytobios 105(410):185–191

    PubMed  CAS  Google Scholar 

  • Yazdanparast R, Bahramikia S (2008) Evaluation of the effect of Anethum graveolens L. crude extracts on serum lipids and lipoproteins profiles in hypercholesterolaemic rats. DARU 16(2):88–94

    CAS  Google Scholar 

  • Zhao QF, Li QX, Ma SR, Cui Y, Wang G (2006) RAPD analysis of genetic diversity of Kobresia humilis along the eastern of Qinghai-Tibet plateau of China. Acta Ecol Sin 26:2494–2501 (in Chinese with English abstract)

    CAS  Google Scholar 

  • Zohary D, Hopf M (2000) Domestication of plants in the Old World. The origin and spread of cultivated plants in West Asia, Europe and the Nile Valley, 3rd edn. Clarendon Press, Oxford

    Google Scholar 

Download references

Acknowledgments

This study was supported by the Agenda project (#PJ0066562010) of the Rural Development Administration (RDA), Republic of Korea.

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Authors and Affiliations

  1. National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Suwon, 441-853, Republic of Korea

    Sundan Suresh, Jong-Wook Chung, Jung-Sook Sung, Gyu-Taek Cho, Jong-Hyun Park, Mun Sup Yoon, Chung-Kon Kim & Hyung-Jin Baek

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  1. Sundan Suresh
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  2. Jong-Wook Chung
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  3. Jung-Sook Sung
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  4. Gyu-Taek Cho
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  6. Mun Sup Yoon
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  7. Chung-Kon Kim
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  8. Hyung-Jin Baek
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Correspondence to Hyung-Jin Baek.

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S. Suresh and J.-W. Chung equally contributed to this work.

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10722_2012_9886_MOESM1_ESM.xlsx

Table S1 Nei’s unbiased measure of genetic distance across 135 accessions of A. graveolens. Supplementary material 1 (XLSX 106 kb)

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Suresh, S., Chung, JW., Sung, JS. et al. Analysis of genetic diversity and population structure of 135 dill (Anethum graveolens L.) accessions using RAPD markers. Genet Resour Crop Evol 60, 893–903 (2013). https://doi.org/10.1007/s10722-012-9886-7

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