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Development and identification of a wheat-Psathyrostachys huashanica addition line carrying HMW-GS, LMW-GS and gliadin genes

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Abstract

Psathyrostachys huashanica Keng, in the family Poaceae, subtribe Triticeae is an endangered and endemic species of China. It is characterized as Shaanxi, drought tolerant and fungus and disease resistant. In this paper, a wheat (Triticum aestivum L.)—P. huashanica disomic addition line “H9021-28-5” was developed using cytogenetics methods including SDS–PAGE, A-PAGE, and GISH. These investigations revealed that the chromosome number and configuration of “H9021-28-5” were 2n = 44 = 22 II. The mitotic and meiotic GISH analysis indicated that “H9021-28-5” contained 42 wheat chromosomes and a pair of P. huashanica chromosomes. The SDS–PAGE and A-PAGE analysis showed that “H9021-28-5” carried the HMW-GS, LMW-GS and gliadin genes of P. huashanica. The results suggest that these storage protein genes of P. huashanica had been transferred into common wheat. Development of this new germplasm is significant for enriching and improving wheat resources of storage proteins genes, and for continuing to exploit the advantageous genes of P. huashanica.

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Abbreviations

A-PAGE:

Acid polyacrylamide gel electrophoresis

GISH:

Genome in situ hybridization

HMW-GS:

High molecular weight glutenin subunit

LMW-GS:

Low molecular weight glutenin subunit

SDS–PAGE:

Sodium dodecyl sulphate polyacrylamide gel electrophoresis

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Acknowledgments

The authors thank Prof. Song Wei-ning and Dr. Zhang Xiao-ke at Northwest A & F University for critically reviewing the manuscript. This research was supported by the National Natural Science Foundation of China (No. 30771345), ‘New Century Talents Support Scheme’ project from Ministry of Education (No. NCET-06-0862), and ‘13115’ Major Innovation Project for Science and Technology of Shaanxi Province (No. 2008ZDKG-07, 2008ZDGC-02).

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Authors and Affiliations

  1. College of Agronomy, Northwest A & F University, 712100, Yangling, Shaanxi, China

    Ji-Xin Zhao, Wan-Quan Ji, Jun Wu, Xin-Hong Chen, Jian-Wei Wang, Yu-Hui Pang, Shu-Hui Liu & Qun-Hui Yang

  2. College of Life Sciences, Northwest A & F University, 712100, Yangling, Shaanxi, China

    Xue-Ni Cheng

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  1. Ji-Xin Zhao
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Correspondence to Xin-Hong Chen.

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Zhao, JX., Ji, WQ., Wu, J. et al. Development and identification of a wheat-Psathyrostachys huashanica addition line carrying HMW-GS, LMW-GS and gliadin genes. Genet Resour Crop Evol 57, 387–394 (2010). https://doi.org/10.1007/s10722-009-9477-4

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