Abstract
For the selection of donors with valuable characteristics for breeding, 39 thyme accessions were evaluated in three years according to a staggered schedule. The criteria investigated were: winter hardiness, beginning of flowering, growth height, yield of the dry herb, content of essential oil, composition of the essential oil, DNA content of cell nuclei and number of chromosomes. The most strongly varying traits between the populations were the yield of dry herb, the content of essential oil and the content of volatile phenols with coefficients of variation (CV) between CV 40% and 50%. The largest variation within a population was detected for the yield of dry herb (CV 25–46%) and the content of essential oil (CV 17–48%). The homogeneity of the populations was different. The minimal average coefficient of variation of all traits (CV 19%) was determined in the population of the cultivar ‘Varico II’ and in a population from Lithuania. The ploidy level of T. vulgaris was diploid (2n = 30).
Similar content being viewed by others
References
Bauer F, Luf W (2002) Antioxidative effects of herbs in meat products II – slow down in fat changes. Institute for Applied Botany, University of Veterinary Medicine, Vienna. Funktionelle Pflanzenstoffe in der Veterinärmedizin, Vienna
Chizzola R (2005) Variabilität im ätherischen Öl von südfranzösischen und spanischen Wildpopulationen des Thymians (Thymus vulgaris L.) und daraus erstellten Feldkulturen. Zeitschrift für Arznei- & Gewürzpflanzen 10(2):82–90
Dachler M, Pelzmann H (1999) Heil- und Gewürzpflanzen - Anbau - Ernte - Aufbereitung. Österreichischer Agrarverlag. Wien
Dommée B, Assouad MW, Valdeyron G (1978) Natural selection and gynodioecy in T. vulgaris L. Bot J Linn Soc 77(1):17–28
Elena-Rosello JA (1981) Cytotaxonomic and evolutionary studies in Thymus (Labiate); relationships of the members of section T. Jalas [Spain, includes chromosome numbers]. Anales – Instituto Botanico AJ Cavarilles 38(1):51–59
Gigord L, Lavigne C, Shykoff JA, Atlan A (1999) Evidence for effects of restorer genes on male and female reproductive functions of hermaphrodites in the gynodioecious species T. vulgaris L. J Evol Biol 12(3):596–604
Granger R, Passet J (1973) T. vulgaris L. spontané de France: races chimiques et chemotaxonomie. Phytochemistry 12:1683–1691
Granger R, Passet J, Teulade-Arbousset G (1963) Diversité des essences de T. vulgaris L. La France et ses Parfums 6:225–230
Hoven R, Zappe H, Zitterl-Eglseer K, Jugl M, Franz C (2003) Study of the effect of Bronchipret on the lung function of five Austrian saddle horses suffering recurrent airway obstruction (heaves). Veterinary 152(18):555–557
Hudaib M, Speroni E, Di Pietra AM, Cavrini V (2002) GC/MS evaluation of thyme (Thymus vulgaris L.) oil composition and variations during the vegetative cycle. J Pharm Biomed Anal 29:691–700
Jalas J (1948) Chromosome studies in Thymus. I. Somatic chromosome numbers with special reference to the fennoscandian forms. Hereditas 34:414–434
Jugl M, Hagmüller W, Zitterl-Eglseer K, Spergser J, Schilcher F, Novak J, Gabler C, Bucher A, Schuh M (2002) Thyme (Thymus vulgaris L.) as feed additive in the rearing period of piglets. 1st European symposium on bioactive secondary plant products in veterinary medicine. October 4–5, 2002, Vienna, Austria, p 21
Kivanc M, Akgul A (1986) Antibacterial activities of essential oils from Turkish spices and citrus. Flavour Frag J 1(4/5):175–179
McClintock B (1929) A method for making acetocarmine smears permanent. Stain Technol 4:53–56
McGimpsey JA, Douglas MH, Van Klinik JW, Beauregard DA, Perry NB (1994) Seasonal variation in essential oil yield and composition from naturalized T. vulgaris L. in New Zealand. Flavour Frag J 9:347–352
Nascimento GGF, Locatelli J, Freitas PC, Silva GL (2000) Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Braz J Microbiol 21(4):247–256
N.N. Thymian Thymi herba (2002) Europäisches Arzneibuch, 4. Ausgabe, 1. Nachtrag. Deutscher Apotheker Verlag, Govi, Stuttgart, Eschborn, pp 3390–3392
Pank F, Krüger H (2003) Sources of variability of thyme populations (Thymus vulgaris L.) and conclusions for breeding. Zeitschrift für Arznei- & Gewürzpflanzen 8:117–124
Pank F, Pfefferkorn A (2005) Präzision und Treffgenauigkeit von Ätherischöl-Gehaltsbestimmungen mit einer modifizierten Destillations-Apparatur für die Arznei- und Gewürzpflanzenzüchtung. Zeitschrift für Arznei- & Gewürzpflanzen 3:146–150
Passet J (1971) Thymus vulgaris L.: Chémotaxonomie et biogenèse monoterpénique. Ph.D. Thesis, Université de Montpellier, Montepellier
Rasch D (1983) Einführung in die Biostatistik. VEB Deutscher Landwirtschaftsverlag Berlin, p 52
Rey C (1995) Comparaison du semis direct et du plant motté pour la mise en place de la sauge officinale (Salvia officinalis L.). Revue suisse Vitic. Arboric. Hortic. 27:375–381
Smith-Palmer A, Stewart J, Fyfe L (1998) Antimicrobial properties of plant essential oils and essences against five important food-borne pathogens. Lett Appl Microbiol 26:118–122
Stahl-Biskup E (2002) Thyme: the genus Thymus. Hamburg. Taylor & Francis, London, UK
Thompson JD (2002) Population structure and the spatial dynamics of genetic polymorphism in thyme. In: Stahl-Biskup E, Sáez F (eds) Thyme the genus Thymus. Medicinal and aromatic plants—industrial profiles, vol 24. Taylor & Francis, New York, pp 44–74
Venskutonis PR (1997) Effect of drying on the volatile constituents of thyme (Thymus vulgaris L.) and sage (Salvia officinalis L.). Food Chem 59(2):219–227
Venskutonis R, Poll L, Larsen M (1996) Influence of drying and irradiation on the composition of volatile compounds of thyme (Thymus vulgaris L.). Flavour Frag J 11(2):123–128
Vernet P, Gouyon PH, Valdeyron G (1986) Genetic control of the oil content in Thymus vulgaris L.: a case of polymorphism in a biosynthetic chain. Genetica 69(3):227–231
Acknowledgements
The authors thank the Federal Ministry of Education and Research and Dr. Junghanns GmbH for the financing of the project, Dr. K. Pistrick (Institute of Plant Genetics and Crop Plant Research, Gatersleben) for assistance in taxonomical assignment and all institutes involved for the supply of the thyme populations.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Mewes, S., Krüger, H. & Pank, F. Physiological, morphological, chemical and genomic diversities of different origins of thyme (Thymus vulgaris L.). Genet Resour Crop Evol 55, 1303–1311 (2008). https://doi.org/10.1007/s10722-008-9329-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10722-008-9329-7