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Genetic diversity and gene-pool subdivisions of diploid D-genome Aegilops tauschii Coss. (Poaceae) in Iran as revealed by AFLP

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Abstract

The diploid goatgrass Aegilops tauschii is considered the D-genome donor of bread wheat and has probably a centre of diversity in north of Iran. In order to measure the genetic diversity of and the relationships among different populations, varieties and subspecies belonging to Ae. tauschii in Iran, DNA was extracted from 48 accessions of Ae. tauschii collected across the geographic range of the species in the Country and the genetic diversity was assessed using AFLPs based on eight PstI/MseI +3 primer pairs resulted in 277 bands, 198 of which were polymorphic. High level polymorphism was detected, with an average of polymorphism rate of 0.715; relatively low genetic similarity (0.455) between accessions and significant difference between the lowest (0.179) and the highest genetic similarity (0.817). The Iranian Ae. tauschii populations showed high level of genetic diversity. The populations studied were divided into two groups: one group was mainly representing Northern populations collected from Southern Caspian Sea shore and the other group was mainly representing Northeast and Northwest populations. Based on the results of this study, it can be suggested that Ae. tauschii possesses two separate gene-pools in Iran: Northern and Northeastern–Northwestern. Considering the needs for introducing new characteristics and alleles for wheat improvement purposes, Ae. tauschii Iranian gene-pool is assumed to be of high importance for more investigation in the future.

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References

  • Barrett BA, Kidwell KK (1998) AFLP-based genetic diversity assessment among wheat cultivars from the pacific northwest. Crop Sci 38:1261–1271

    CAS  Google Scholar 

  • Bassam BJ, Caetano-Anolles G, Greesshoff PM (1991) Fast and sensitive silver staining of DNA in polyacrylamide gels. Ann Biochem 19:680–683

    Google Scholar 

  • Chen J, Devanand PS, Norman DJ, Henny RJ, Chao CT (2004) Genetic relationships of Aglaonema species and cultivars inferred from AFLP markers. Ann Bot 93:157–166

    Article  PubMed  CAS  Google Scholar 

  • Dudnikov AJ (2000) Multivariate analysis of genetic variation in Aegilops tauschii from the world germplasm collection. Genet Resour Crop Evol 47:185–190

    Article  Google Scholar 

  • Dvorak J, Luo M-C, Yng Z-L, Zhang H-B (1998) The structure of the Aegilops tauschii genepool and the evolution of hexaploid wheat. Theor Appl Genet 97:657–670

    Article  CAS  Google Scholar 

  • Eig A (1929) Monographisch-kritische Übersicht der Gattung Aegilops. Feddes Repertorium Specierum Novarum Regni Vegetabilis Beih 55:1–228

    Google Scholar 

  • Gawel NJ, Jarret RL (1991) A modified CTAB extraction procedure for Musa and Ipomoea. Plant Mol Biol Rep 9(3):262–266

    Article  CAS  Google Scholar 

  • Gill BS, Raup WI, Sharma HC, Browder LE, Hatchett JH, Harvey TL, Moseman JG, Waines JG (1986) Resistance in Aegilops tauschii to wheat leaf rust, wheat powdery mildew, greenbug, and Hessian fly. Plant Dis 70:550–556

    Article  Google Scholar 

  • Gororo NN, Eagles HA, Eastwood RF, Nicolas ME, Flood RG (2002) Use of Triticum tauschii to improve yield of wheat in low-yielding environments. Euphytica 123:241–254

    Article  Google Scholar 

  • Hammer K (1980) Vorarbeiten zur monographischen Darstellung von Wildpflanzensortimenten: Aegilops L. Kulturpflanze 28:33–180

    Article  Google Scholar 

  • Hazen SP, Leroy P, Ward RW (2002) AFLP in Triticum aestivum L.: patterns of genetic diversity and genome distribution. Euphytica 125:89–102

    Article  CAS  Google Scholar 

  • Jaaska V (1981) Aspartate aminotransferase and alcohol dehydrogenase isoenzyme: intraspecific differentiation in Aegilops tauschii and the origin of the D genome polyploids in the wheat group. Plant Syst Evol 137:259–273

    Article  CAS  Google Scholar 

  • Jaccard P (1908) Nouvelles rescherches sur la distribution florale. Bull Soc Vaud Sci Nat 44:223–270, cited and implemented in NTSYSpc program, version 2.02e

    Google Scholar 

  • Jones CJ, Edwards KJ, Castaglione S, Winfield MO, Sala F, Wiel C, Bredemeijer G, Vosman B, Matthes M, Maly A, Brettschneider R, Bettini P, Buiatti M, Maestri E, Malcevschi A, Marmiroli N, Aert R, Volckaert G, Rueda J, Linaacero R, Vazque A, Karp A (1997) Reproducibility testing of RAPD, AFLP and SSR markers in plants by a network of European laboratories. Mol Breed 3:381–390

    Article  CAS  Google Scholar 

  • Kerber ER, Dyck PL (1969) Inheritance in hexaploid wheat of leaf rust resistance and other characters derived from Aegilops squarrosa. Can J Genet Cytol 11:639–647

    Google Scholar 

  • Knaggs P, Ambrose MJ, Reader S, Miller TE (2000) Morphological characterization and evaluation of the subdivision of Aegilops tauschii Coss. Wheat Inf Serv 91:15–19

    Google Scholar 

  • Le HT, Reicosky DA, Olien CR, Cress CE (1986) Freezing hardiness of some accessions of Triticum tauschii and Triticum turgidum var. durum. Can J Plant Sci 66:893–899

    Article  Google Scholar 

  • Lelley T, Stachel M, Grausgruber H, Vollmann J (2000) Analysis of relationships between Aegilops tauschii and the D genome of wheat utilizing microsatellites. Genome 43(4):661–668

    Article  PubMed  CAS  Google Scholar 

  • Limin AE, Fowler DB (1981) Cold hardiness of some wild relatives of hexaploid wheat. Can J Bot 59:572–573

    Article  Google Scholar 

  • Lubbers EL, Gill KS, Cox TS, Gill BS (1991) Variation of molecular markers among geographically diverse accessions of Triticum tauschii. Genome 34:354–361

    Google Scholar 

  • MacKill DJ, Zhang Z, Redona ED, Colowit PM (1996) Level of polymorphism and genetic mapping of AFLP markers in rice. Genome 39:969–977

    Article  PubMed  CAS  Google Scholar 

  • Malik R, Smith CM, Brown-Guedira GL, Harvey TL, Gill BS (2003) Assessment of Aegilops tauschii for resistance to biotypes of wheat Curl Mite (Acari: Eriophydae). J Econ Entomol 96(4):1329–1333

    Article  PubMed  Google Scholar 

  • Margis R, Felix D, Caldas JF, Salgueiro F, De Araujo DSD, Breyne P, Van Montagu M, De Oliviera D, Margis-Pinheiro M (2002) Genetic differentiation among three neighboring Brazil-cherry (Eugenia uniflora L.) populations within the Brazilian Atlantic rain forest. Biodivers Conserv 11:149–163

    Article  Google Scholar 

  • Nakai Y (1979) Isosyme variation in Aegilops and Triticum, IV The origin of the common wheats revealed from the study on esterase isozymes in synthesized wheats. Jpn J Genet 54:175–189

    Article  Google Scholar 

  • Nishikawa K, Furuta Y, Wada T (1980) Genetic studies on alpha-amylase isozymes in wheat. III. Intraspecific variation in Aegilops squarrosa and the birthplace of hexaploid wheat. Jpn J Genet 55:325–336

    Article  Google Scholar 

  • Pestsova E, Korzun V, Goncharov NP, Hammer K, Ganal MW, Röeder MS (2000) Microsatellite analysis of Aegilops tauschii germplasm. Theor Appl Genet 101:100–106

    Article  CAS  Google Scholar 

  • Rejesus R, van Ginkel M, Smale M (1996) Wheat breeders’ perspectives on genetic diversity and germplasm use: findings from an international survey. Plant Var Seeds 9(3):129–147

    Google Scholar 

  • Rohlf FJ (1998) NTSYS-PC numerical taxonomy and multivariate analysis system, version 2.02. Exter Publications, Setauket, NY

    Google Scholar 

  • Saeidi H, Rahiminejad MR, Vallian S, Heslop-Harrison JS (2006) Biodiversity of diploid D-genome Aegilops tauschii Coss. in Iran measured using microsatellites. Genet Resour Crop Evol 53:1477–1484

    Article  Google Scholar 

  • Shah SH, Gorham J, Forster BP, Wyn Jones RG (1987) Salt tolerance in the Triticeae: The contribution of the D genome to cation selectivity in hexaploid wheat. J Exp Bot 38:254–269

    Article  CAS  Google Scholar 

  • Slageren MW (1994) Wild wheats: a monograph of Aegilops L. and Amblyopyrum (Jaub. & Spach) Eig. Wageningen Agricultural University

  • Tanaka M (1983) Geographical distribution of Aegilops species based on the collections at the Plant Germ-plasm Institute, Kyoto University. Proc. 6th international wheat genetics symposium, Kyoto, Japan, pp 1009–1024

  • Tang T, Zhong Y, Jian S, Shi S (2003) Genetic diversity of Hibiscus tiliaceus (Malvaceae) in China assessed using AFLP markers. Ann Bot 92:409–414

    Article  PubMed  CAS  Google Scholar 

  • Tanksley SD, McCouch SR (1997) Seed banks and molecular maps: unlocking genetic potential from the wild. Science 277:1063–1066

    Article  PubMed  CAS  Google Scholar 

  • Tsunewaki K (1966) Comparative gene analysis of common wheat and its ancestral species. II. Waxiness, growth habit and awnedness. Jpn J Bot 19:175–229

    Google Scholar 

  • Vavilov NI (1940) The theory of the origin of cultivated plants after Darwin. Nauka (Science) 2:55–75

    Google Scholar 

  • Voorrips RE, Jongerius MC, Kanne HJ (1997) Mapping of two genes for resistance to clubroot (Plasmodiophora brassicae) in a population of doubled haploid lines of Brassica oleracea by means of RFLP and AFLP markers. Theor Appl Genet 94:75–82

    Article  CAS  PubMed  Google Scholar 

  • Vos P, Hogres R, Bleeker M, Reijans M, Lee T, Hornes M, Frilters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA finger printing. Nucleic Acids Res 23:4407–4414

    Article  PubMed  CAS  Google Scholar 

  • Zhu J, Gale MD, Quarrie S, Jackson MT, Bryan GJ (1998) AFLP markers for the study of rice biodiversity. Theor Appl Genet 96:602–611

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran

    Hojjatollah Saeidi & Mohammad Reza Rahiminejad

  2. Department of Agricultural Biotechnology, Isfahan University of Technology, Isfahan, Iran

    Badradin Ebrahim Sayed Tabatabaei, Mehdi Rahimmalek & Majid Talebi-Badaf

  3. Department of Agronomy and Plant Breeding, Faculty of Agriculture, Azad University of Khorasgan, Isfahan, Iran

    Mehdi Rahimmalek

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  1. Hojjatollah Saeidi
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  2. Badradin Ebrahim Sayed Tabatabaei
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  3. Mehdi Rahimmalek
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  4. Majid Talebi-Badaf
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  5. Mohammad Reza Rahiminejad
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Correspondence to Mohammad Reza Rahiminejad.

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Saeidi, H., Sayed Tabatabaei, B.E., Rahimmalek, M. et al. Genetic diversity and gene-pool subdivisions of diploid D-genome Aegilops tauschii Coss. (Poaceae) in Iran as revealed by AFLP. Genet Resour Crop Evol 55, 1231–1238 (2008). https://doi.org/10.1007/s10722-008-9323-0

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  • DOI: https://doi.org/10.1007/s10722-008-9323-0

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