Abstract
This work studied the combined use of gliadins and SSRs to analyse inter- and intra-accession variability of the Spanish collection of cultivated einkorn (Triticum monococcum L. ssp. monococcum) maintained at the CRF-INIA. In general, gliadin loci presented higher discrimination power than SSRs, reflecting the high variability of the gliadins. The loci on chromosome 6A were the most polymorphic with similar PIC values for both marker systems, showing that these markers are very useful for genetic variability studies in wheat. The gliadin results indicated that the Spanish einkorn collection possessed high genetic diversity, being the differentiation large between varieties and small within them. Some associations between gliadin alleles and geographical and agro-morphological data were found. Agro-morphological relations were also observed in the clusters of the SSRs dendrogram. A high concordance was found between gliadins and SSRs for genotype identification. In addition, both systems provide complementary information to resolve the different cases of intra-accession variability not detected at the agro-morphological level, and to identify separately all the genotypes analysed. The combined use of both genetic markers is an excellent tool for genetic resource evaluation in addition to agro-morphological evaluation.
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Aguiriano E, Ruiz M, Fité R, Carrillo JM (2006) Analysis of genetic variability in a sample of the durum wheat Spanish collection based on gliadin markers. Genet Resour Crop Evol 53:1543–1552
Alvarez JB, Moral A, Martín LM (2006) Polymorphism and genetic diversity for the seed storage proteins in Spanish cultivated einkorn wheat (Triticum monococcum L. ssp. monococcum). Genet Resour Crop Evol 53:1061–1067
Anderson JA, Churchill GA, Antrique JE, Tanksley SD, Sorrels ME (1993) Optimising parental selection for genetic linkage maps. Genome 36:181–188
Bohn M, Utz HF, Melchinger AE (1999) Genetic similarities among winter wheat cultivars determined on the basis of RFLPs, AFLPs, and SSRs and their use for predicting progeny variance. Crop Sci 39:228–237
Castagna R, Maga G, Perenzin M, Heun M, Salamini F (1994) RFLP-based genetic relationship of Einkorn wheats. Theor Appl Genet 88:818–823
Ciaffi M, Dominici L, Lafiandra D (1997) Gliadin polymorphism in wild and cultivated einkorn wheats. Theor Appl Genet 94:68–74
Dorofeev VF, Korovina ON (eds) (1979) Wheat. Flora of Cultivated Plants (Russ., Engl. summary). ‘Kolos’, Leningrad
Flaksberger KA (1935) Wheat. Flora of Cultivated Plants (Russ) Vol I, Leningrad
Fedak G (1985) Alien species as sources of physiological traits for wheat improvement. Euphytica 34:673–680
Ghosh S, Karanjawala ZE, Hauser ER, Ally D, Knapp JI, Rayman JB, Musick A, Tannenbaum J, Te C, Shapiro S (1997) Methods for precise sizing, automated binning of alleles, and reduction of error rates in large-scale genotyping using fluorescently labeled dinucleotide markers. Genome Res 7:165–178
Goldstein DB, Pollock DD (1997) Launching microsatellites: a review of mutation processes and method for phylogenetic inference. Genetics 139:463–471
Hammer K, Filatenko AA, Korzun V (2000) Microsatellite markers – a new tool for distinguishing diploid wheat species. Genet Resour Crop Evol 47:497–505
Hardy OJ, Vekemans X (2002) SPAGeDi: a versatile computer program to analyse spatial genetic structure at the individual or population levels. Mol Ecol Notes 2:618–620
Huang XQ, Börner A, Röder MS, Ganal MW (2002) Assessing genetic diversity of wheat (Titicum aestivum L.) germplasm using microsatellite markers. Theor Appl Genet 106:699–707
IBPGR (1985) Revised Descriptor list for wheat (Triticum ssp). International Board for Plant Genetic Resources, Rome, Italy
Kudryavtsev AM, Boggini G, Benedettelli S, Illichevskii NN (1996) Gliadin polymorphism and genetic diversity of modern Italian durum wheat. J Genet Breed 50:239–248
Kuspira J, Maclagan J, Bhambhani RN, Sadasivaiah RS, Kim NS (1989) Genetic and cytogenetic analyses of the A genome of T. monococcum L. V. Inheritance and linkage relationships of genes determining the expression of 12 qualitative characters. Genome 32:869–881
Lafiandra D, Kasarda DD (1985) One and two-dimensional (two-pH) polyacrylamide gel electrophoresis in a single gel: separation of wheat proteins. Cereal Chem 62:314–319
Le Corre V, Bernad M (1995) Assessment of the type and degree of restriction fragment length polymorphism (RFLP) in diploid species of the genus Triticum. Theor Appl Genet 90:1063–1067
Mantel N (1967) The detection of disease clustering and a generalized regression approach. Cancer Res 27:209–220
Medini M, Hamza S, Rebai A, Baum M (2005) Analysis of gentic diversity in Tunisian durum wheat cultivars and related wild species by SSR and AFLP markers. Genet Resour Crop Evol 52:21–31
Metakovsky EV, Baboev SK (1992) Polymorphism and inheritance of gliadin polypeptides in T. monococcum L. Theor Appl Genet 84:971–978
Metakovsky EV, Novoselskaya AY, Kopus MM, Sobko TA, Sozinov AA (1984) Blocks of gliadin components in winter wheat detected by one-dimensional polyacrylamide gel electrophoresis. Theor Appl Genet 67:559–568
Nei M (1973) Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci USA 70:3321–3323
Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023
Rogers JS (1972) Measures of genetic similarity and genetic distance. In: Studies in Genetics VII. University of Texas, Austin, TX, pp 145–153
Rohlf FJ (1992) NTSYS-pc Numerical taxonomy and multivariate analysis system. Exeter software, NewYork
Ruiz M, Rodriguez-Quijano M, Metakovsky EV, Vazquez F, Carrillo JM (2002) Polymorphism, variation and genetic identity of Spanish common wheat germplasm based on gliadin alleles. Field Crops Res 79:185–196
Saghai-Maroof MA, Soliman K, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphism in barley: Mendelian inheritance, chromosomal location, and population dynamics. PNAS 81:8014–8018
Saponaro C, Pogna NE, Castagna R, Pasquín M, Cacciatori P, Redaelli R (1995) Allelic variation at the Gli-A1 m, Gli-A2 m and Glu-A1 m loci and breadmaking quality in diploid wheat Triticum monococcum. Genet Res Camb 66:127–137
Szabó AT, Hammer K (1996) Notes on the taxonomy of farro: Triticum monococcum, T. dicoccon and T. spelta. In: Padulosi S, Hammer K, Heller J (eds) Hulled wheats. Proc. First Int. Workshop. Castelvecchio Pascoli, pp 2–40
The TT (1973) Chromosome location of genes conditioning stem rest resistance transferred from diploid to hexaploid wheat. Nature New Biol 241:256
Vallega V (1992) Agronomical performance and breeding value of selected strains of diploid wheat, Triticum monococcum L. Euphytica 61:13–23
Vierling RA, Nguyen HT (1992) Use of RAPD markers to determine the genetic diversity of diploid wheat genotypes. Theor Appl Genet 84:835–838
Wright S (1978) Evolution and the genetics of populations, vol 4. Variability within and among natural populations. University of Chicago Press, Chicago
Acknowledgements
This work was supported by the grant RFP2004-018 from the INIA of Spain and the AGL 2003-6382 from Comision Interministerial de Ciencia y Tecnología (CICYT) of Spain. E. Aguiriano has received a pre-doctoral scholarship from the INIA.
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Ruiz, M., Aguiriano, E., Fité, R. et al. Combined use of gliadins and SSRs to analyse the genetic variability of the Spanish collection of cultivated diploid wheat (Triticum monococcum L. ssp. monococcum). Genet Resour Crop Evol 54, 1849–1860 (2007). https://doi.org/10.1007/s10722-007-9208-7
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DOI: https://doi.org/10.1007/s10722-007-9208-7