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Analysis of genetic diversity in the endangered tropical tree species Hagenia abyssinica using ISSR markers

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Abstract

Genetic diversity within and among 12 populations of the dioecious tropical tree species Hagenia abyssinica (Bruce) J.F. Gmel. in Ethiopia was examined with eight inter simple sequence repeat (ISSR) primers. A total of 104 clearly scorable bands were generated, among which 84 (81%) were polymorphic. Jaccard similarity coefficient was calculated for pairwise comparisons among all 120 individuals and ranged from 0.30 to 0.88 while average within-population similarity ranged from 0.53 to 0.66. Within-population variability was estimated as percentage polymorphic loci (ranging from 52% to 87%), Shannon’s information index (0.30–0.50) and Nei’s genetic diversity (0.21–0.35). The highest variability values were obtained for one recently planted population and for one wild population growing in an undisturbed primary forest area. Significant overall differentiation among populations was detected by both Shannon’s information index (0.26) and G ST (0.25). Relatedness among samples was estimated with a principal coordinate analysis, and relatedness among populations was estimated with a cluster analysis (UPGMA). A Mantel test indicated a significant association between genetic and geographic distances, and an autocorrelation analysis showed significant evidence of gene flow over distances up to 30 km. This study is the first of its kind for H. abyssinica, which has decreased recently in Ethiopia and now must be regarded as an endangered species. Both within-population and between-population diversity estimates are typical of outcrossing, longlived and late successional species, suggesting that recent anthropogenic disturbances have not yet had much impact on population genetic parameters. DNA marker data can, however, be used to identify the most suitable sites for in situ conservation and for collection of material for establishment of genebanks and plant improvement programs.

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Acknowledgements

This work was supported by BIO-EARN (East African Regional Programme and Research Network for Biotechnology, Biosafety and Biotechnology Policy Development) funded by SIDA/SAREC, Sweden. The authors acknowledge Department of Biology, Addis Ababa University for the help during sample collections from the field.

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, Addis Ababa University, 1176, Addis Ababa, Ethiopia

    Tileye Feyissa

  2. Balsgård – Department of Crop Science, Swedish University of Agricultural Sciences, Fjälkestadsvägen 459, SE-291 94, Kristianstad, Sweden

    Hilde Nybom

  3. Department of Phanerogamic Botany, Swedish Museum of Natural History, 50007, SE-104 05, Stockholm, Sweden

    Igor V. Bartish

  4. Department of Crop Science, Swedish University of Agricultural Sciences, 44, SE-230 53, Alnarp, Sweden

    Tileye Feyissa & Margareta Welander

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  1. Tileye Feyissa
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  2. Hilde Nybom
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  3. Igor V. Bartish
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  4. Margareta Welander
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Correspondence to Tileye Feyissa.

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Feyissa, T., Nybom, H., Bartish, I.V. et al. Analysis of genetic diversity in the endangered tropical tree species Hagenia abyssinica using ISSR markers. Genet Resour Crop Evol 54, 947–958 (2007). https://doi.org/10.1007/s10722-006-9155-8

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