Abstract
The potential use of RAPD markers in identifying genuine intergeneric hybrids of Saccharum was examined in 12 hybrids of Saccharum with Erianthus spp., Sorghum bicolor and Zea mays. In the three groups of hybrids examined, RAPD markers could precisely identify the true hybrids of Saccharum officinarum × Sorghum bicolor and Saccharum officinarum × Zea mays. Though Erianthus had a very distinct RAPD profile and as many as 107 Erianthus-specific bands were identified, a decisive profile that can identify all the hybrids could not be obtained. The strong molecular differentiation between S.officinarum and Erianthus was not adequately resolved in the RAPD profiles of their hybrid progenies which suggest that more refined molecular techniques will be required for the characterization of Saccharum–Erianthus hybrids.
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K. Alix F.C. Baurens F. Paulet J.C. Glaszmann A. D’Hont (1998) ArticleTitleIsolation and characterization of a satellite DNA family in the Saccharum complex Genome 41 854–864 Occurrence Handle10.1139/gen-41-6-854 Occurrence Handle1:CAS:528:DyaK1MXotValug%3D%3D Occurrence Handle9924794
K. Alix F. Paulet J.C. Glaszmann A. D’Hont (1999) ArticleTitleInter-Alu like sequences in Saccharum complex Theor. Appl. Genet. 99 962–968 Occurrence Handle10.1007/s001220051403 Occurrence Handle1:CAS:528:DyaK1MXotVCis7o%3D
P. Besse C.L. Mc Intyre N. Berding (1996) ArticleTitleRibosomal DNA variations in Erianthus, a wild sugarcane relative (Andropogonae–Saccharinae) Theor. Appl. Genet. 92 733–743 Occurrence Handle1:CAS:528:DyaK28XltFOjs74%3D
P. Besse C.L. Mc Intyre (1998) ArticleTitleIsolation and characterization of repeated DNA sequences from Erianthus spp. (Saccharinae:Andropogonae) Genome 41 408–416 Occurrence Handle10.1139/gen-41-3-408 Occurrence Handle1:CAS:528:DyaK1cXlvVCrsb0%3D
A. D’Hont P.S. Rao P. Feldmann L. Grivet N. Islam-Faridi P. Taylor J.C. Glaszmann (1995) ArticleTitleIdentification and characterization of sugarcane intergeneric hybrids, Saccharum officinarum × Erianthus arundinaceuswith molecular markers and DNA in situ hybridization Theor. Appl. Genet. 91 320–326 Occurrence Handle1:CAS:528:DyaK2MXotl2qtLY%3D
E.K. Janakiammal (1938) ArticleTitleA Saccharum–Zea cross Nature 142 618–619
E.K. Janakiammal (1941) ArticleTitleIntergeneric hybrids of Saccharum J. Genet. 41 217–253
Janakiammal E.K. and Singh T.S.N. 1936. A preliminary work on a new Saccharum–Sorghum hybrid. Indian J. Agric. Sci. 1105–1106.
H.W. Li C.S. Loh C.L. Lee (1948) ArticleTitleHybrids between Saccharum officinarum, Miscanthus japonicus and S. spontaneum Bot. Bull. Acad. Sin. (Taipei) 2 147–160
N.V. Nair (1999) ArticleTitleProduction and cytomorphological analysis of intergeneric hybrids of Sorghum × Saccharum Euphytica 108 187–191 Occurrence Handle10.1023/A:1003633015836
S. Price (1967) ArticleTitleInterspecific hybridization in sugarcane Breeding Proc. Int. Soc. Sugarcane Technol. 12 1021–1026
R. Thomas T.S. Venkatraman (1930) ArticleTitleSugarcane-Sorghum hybrids Agric. J. India 25 164
V. Walbot (1988) ArticleTitlePreparation of DNA from single rice seedlings Rice Genet. Newsl. 5 149–151
J.G.K. Williams A.R. Kubelik K.J. Livak J.A. Rafalski S.V. Tingey (1990) ArticleTitleDNA polymorphisms amplified by arbitrary primers are useful as genetic markers Nucleic Acids Res. 18 6531–6535 Occurrence Handle1:CAS:528:DyaK3MXjslWmsA%3D%3D Occurrence Handle1979162
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Nair, N.V., Selvi, A., Sreenivasan, T.V. et al. Characterization of Intergeneric Hybrids of Saccharum using Molecular Markers. Genet Resour Crop Evol 53, 163–169 (2006). https://doi.org/10.1007/s10722-004-1810-3
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DOI: https://doi.org/10.1007/s10722-004-1810-3