Skip to main content
SpringerLink
Log in

Structural characterization and biological activities of a new polysaccharide isolated from Morchella Sextelata

  • Original Article
  • Published:
Glycoconjugate Journal Aims and scope Submit manuscript

Abstract

Morchella is the famous medicinal fungi in the ascomycetes. In this study, a new water-soluble polysaccharide (MSP-3-1) with an average molecular weight of 2.35 × 107 Da was extracted and purified from fruiting bodies of cultivated M. Sextelata. The structural characterization and biological activities of purified polysaccharide was further investigated. The results indicated that MSP-3-1 was mainly a α-glucan, mainly consisting of mannose (Man), glucose (Glc) and galactose (Gal) in a ratio of 5.10: 91.39: 3.51. Its surface morphology exhibited irregular lamellar structures with small voids. And the particle size analysis showed that MSP-3-1 was the homogeneous nanoparticle in water solution. Furthermore, the antioxidant activity analysis showed that MSP-3-1 possessed certain scavenging activity against hydroxyl radicals, DPPH radicals and ABTS radicals in a dose-dependent manner. Immunological tests suggested that MSP-3-1 could significantly promote the proliferation, phagocytosis and nitric oxide (NO) production of macrophage RAW264.7. Thus, our results will provide a theoretical basis for the development and utilization of Morchella Sextelata polysaccharides as an immunmodulatory component in functional foods.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

Similar content being viewed by others

Data availability

All data generated or analysed during this study are included in this published article.

References

  1. Tietel, Z., Masaphy, S.: True morels (Morchella)-nutritional and phytochemical composition, health benefits and flavor: A review. Crit. Rev. Food. Sci. Nutr. 58(11), 1888–1901 (2018). https://doi.org/10.1080/10408398.2017.1285269

    Article  CAS  PubMed  Google Scholar 

  2. Xiong, C., Li, P., Luo, Q., Yan, J., Zhang, J., Jin, X., Huang, W.: Effect of γ-irradiation on the structure and antioxidant activity of polysaccharide isolated from the fruiting bodies of Morchella sextelata. Biosci. Rep. 40(9), BSR0194522 (2020). https://doi.org/10.1042/BSR20194522

  3. Wen, Y., Peng, D., Li, C., Hu, X., Bi, S., Song, L., Peng, B., Zhu, J., Chen, Y., Yu, R.: A new polysaccharide isolated from Morchella importuna fruiting bodies and its immunoregulatory mechanism. Int. J. Biol. Macromol. 137, 8–19 (2019). https://doi.org/10.1016/j.ijbiomac.2019.06.171

    Article  CAS  PubMed  Google Scholar 

  4. Badshah, S.L., Riaz, A., Muhammad, A., Tel Cayan, G., Cayan, F., Emin Duru, M., Ahmad, N., Emwas, A.H., Jaremko, M.: Isolation, Characterization, and Medicinal Potential of Polysaccharides of Morchella esculenta. Molecules. 26(5), 1459 (2021). https://doi.org/10.3390/molecules26051459

  5. Cheng, H., Huang, G.: Extraction, characterisation and antioxidant activity of Allium sativum polysaccharide. Int. J. Biol. Macromol. 114, 415–419 (2018). https://doi.org/10.1016/j.ijbiomac.2018.03.156

    Article  CAS  PubMed  Google Scholar 

  6. Huang, X., Nie, S.: The structure of mushroom polysaccharides and their beneficial role in health. Food. Funct. 6(10), 3205–3217 (2015). https://doi.org/10.1039/c5fo00678c

    Article  CAS  PubMed  Google Scholar 

  7. Yan, M.X., Mao, W.J., Liu, X., Wang, S.Y., Xia, Z., Cao, S.J., Li, J., Qin, L., Xian, H.L.: Extracellular polysaccharide with novel structure and antioxidant property produced by the deep-sea fungus Aspergillus versicolor N2bc. Carbohydr. Polym. 147, 272–281 (2016). https://doi.org/10.1016/j.carbpol.2016.03.090

    Article  CAS  PubMed  Google Scholar 

  8. Sun, H., Zhang, J., Chen, F., Chen, X., Zhou, Z., Wang, H.: Activation of RAW264.7 macrophages by the polysaccharide from the roots of Actinidia eriantha and its molecular mechanisms. Carbohydr. Polym. 121, 388–402 (2015). https://doi.org/10.1016/j.carbpol.2014.12.023

  9. He, P., Dong, Z., Wang, Q., Zhan, Q.P., Zhang, M.M., Wu, H.: Structural Characterization and Immunomodulatory Activity of a Polysaccharide from Eurycoma longifolia. J. Nat. Prod. 82(2), 169–176 (2019). https://doi.org/10.1021/acs.jnatprod.8b00238

    Article  CAS  PubMed  Google Scholar 

  10. Rong, L., Li, G., Zhang, Y., Xiao, Y., Qiao, Y., Yang, M., Wei, L., Bi, H., Gao, T.: Structure and immunomodulatory activity of a water-soluble alpha-glucan from Hirsutella sinensis mycelia. Int J Biol Macromol 189, 857–868 (2021). https://doi.org/10.1016/j.ijbiomac.2021.08.185

    Article  CAS  PubMed  Google Scholar 

  11. Wu, J.-Y., Siu, K.-C., Geng, P.: Bioactive Ingredients and Medicinal Values of Grifola frondosa (Maitake). Foods 10(1), 95 (2021). https://doi.org/10.3390/foods10010095

  12. Wang, J., Hu, S., Nie, S., Yu, Q., Xie, M.: Reviews on Mechanisms of In Vitro Antioxidant Activity of Polysaccharides. Oxid. Med. Cell. Longev. 2016, 5692852 (2016). https://doi.org/10.1155/2016/5692852

    Article  CAS  PubMed  Google Scholar 

  13. Zhang, H., Zou, P., Zhao, H., Qiu, J., Regenstein, J.M., Yang, X.: Isolation, purification, structure and antioxidant activity of polysaccharide from pinecones of Pinus koraiensis. Carbohydr. Polym. 251, 117078 (2021). https://doi.org/10.1016/j.carbpol.2020.117078

    Article  CAS  PubMed  Google Scholar 

  14. Pan, Q., Sun, Y., Li, X., Zeng, B., Chen, D.: Extraction, structural characterization, and antioxidant and immunomodulatory activities of a polysaccharide from Notarchus leachii freeri eggs. Bioorg. Chem. 116, 105275 (2021). https://doi.org/10.1016/j.bioorg.2021.105275

    Article  CAS  PubMed  Google Scholar 

  15. Liu, J., Wang, Y., Wu, J., Georgiev, M.I., Xu, B., Wong, K.H., Bai, W., Tian, L.: Isolation, Structural Properties, and Bioactivities of Polysaccharides from Mushrooms Termitomyces: A Review. J. Agric. Food. Chem. 70(1), 21-33(2022). https://doi.org/10.1021/acs.jafc.1c06443

    Article  PubMed  Google Scholar 

  16. Anusiya, G., Gowthama Prabu, U., Yamini, N.V., Sivarajasekar, N., Rambabu, K., Bharath, G., Banat, F.: A review of the therapeutic and biological effects of edible and wild mushrooms. Bioengineered 12(2), 11239–11268 (2021). https://doi.org/10.1080/21655979.2021.2001183

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Wen, Y., Bi, S., Hu, X., Yang, J., Li, C., Li, H., Yu, D.B., Zhu, J., Song, L., Yu, R.: Structural characterization and immunomodulatory mechanisms of two novel glucans from Morchella importuna fruiting bodies. Int. J. Biol. Macromol. 183, 145–157 (2021). https://doi.org/10.1016/j.ijbiomac.2021.04.084

    Article  CAS  PubMed  Google Scholar 

  18. Cai, Z.N., Li, W., Mehmood, S., Pan, W.J., Wang, Y., Meng, F.J., Wang, X.F., Lu, Y.M., Chen, Y.: Structural characterization, in vitro and in vivo antioxidant activities of a heteropolysaccharide from the fruiting bodies of Morchella esculenta. Carbohydr. Polym. 195, 29–38 (2018). https://doi.org/10.1016/j.carbpol.2018.04.069

    Article  CAS  PubMed  Google Scholar 

  19. Meng, X., Che, C., Zhang, J., Gong, Z., Si, M., Yang, G., Cao, L., Liu, J.: Structural characterization and immunomodulating activities of polysaccharides from a newly collected wild Morchella sextelata. Int. J. Biol. Macromol. 129, 608–614 (2019). https://doi.org/10.1016/j.ijbiomac.2019.01.226

    Article  CAS  PubMed  Google Scholar 

  20. Liu, Q., Ma, H., Zhang, Y., Dong, C.: Artificial cultivation of true morels: current state, issues and perspectives. Crit. Rev. Biotechnol. 38(2), 259–271 (2018). https://doi.org/10.1080/07388551.2017.1333082

    Article  PubMed  Google Scholar 

  21. Zhang, F., Zhang, X., Guo, S., Cao, F., Zhang, X., Wang, Y., Liu, J., Qian, B., Yan, Y., Chen, P., Xu, C., Liu, C., Qian, D., Duan, J.A.: An acidic heteropolysaccharide from Lycii fructus: Purification, characterization, neurotrophic and neuroprotective activities in vitro. Carbohydr. Polym. 249, 116894 (2020). https://doi.org/10.1016/j.carbpol.2020.116894

    Article  CAS  PubMed  Google Scholar 

  22. Yan, L., Xiong, C., Xu, P., Zhu, J., Yang, Z., Ren, H., Luo, Q.: Structural characterization and in vitro antitumor activity of A polysaccharide from Artemisia annua L. (Huang Huahao). Carbohydr. Polym. 213, 361–369 (2019). https://doi.org/10.1016/j.carbpol.2019.02.081

  23. Gu, J., Zhang, H., Zhang, J., Wen, C., Zhou, J., Yao, H., He, Y., Ma, H., Duan, Y.: Optimization, characterization, rheological study and immune activities of polysaccharide from Sagittaria sagittifolia L. Carbohydr. Polym. 246, 116595(2020). https://doi.org/10.1016/j.carbpol.2020.116595

  24. Zhang, L., Hu, Y., Duan, X., Tang, T., Shen, Y., Hu, B., Liu, A., Chen, H., Li, C., Liu, Y.: Characterization and antioxidant activities of polysaccharides from thirteen boletus mushrooms. Int. J. Biol. Macromol. 113, 1–7 (2018). https://doi.org/10.1016/j.ijbiomac.2018.02.084

    Article  CAS  PubMed  Google Scholar 

  25. Guo, M.Z., Meng, M., Duan, S.Q., Feng, C.C., Wang, C.L.: Structure characterization, physicochemical property and immunomodulatory activity on RAW264.7 cells of a novel triple-helix polysaccharide from Craterellus cornucopioides. Int. J. Biol. Macromol. 126, 796–804 (2019). https://doi.org/10.1016/j.ijbiomac.2018.12.246

  26. Evans, K.O., Skory, C., Compton, D.L., Cormier, R., Cote, G.L., Kim, S., Appell, M.: Development and Physical Characterization of alpha-Glucan Nanoparticles. Molecules. 25(17), 3807 (2020). https://doi.org/10.3390/molecules25173807

  27. He, P., Geng, L., Mao, D., Xu, C.: Production, characterization and antioxidant activity of exopolysaccharides from submerged culture of Morchella crassipes. Bioprocess. Biosyst. Eng. 35(8), 1325–1332 (2012). https://doi.org/10.1007/s00449-012-0720-6

    Article  CAS  PubMed  Google Scholar 

  28. Gu, J., Zhang, H., Wen, C., Zhang, J., He, Y., Ma, H., Duan, Y.: Purification, characterization, antioxidant and immunological activity of polysaccharide from Sagittaria sagittifolia L. Food. Res. Int. 136, 109345 (2020). https://doi.org/10.1016/j.foodres.2020.109345

    Article  CAS  PubMed  Google Scholar 

  29. Huang, Y., Nan, L., Xiao, C., Ji, Q., Li, K., Wei, Q., Liu, Y., Bao, G.: Optimum Preparation Method for Self-Assembled PEGylation Nano-Adjuvant Based on Rehmannia glutinosa Polysaccharide and Its Immunological Effect on Macrophages. Int. J. Nanomed. 14, 9361–9375 (2019). https://doi.org/10.2147/IJN.S221398

    Article  CAS  Google Scholar 

  30. Nie, C., Zhu, P., Ma, S., Wang, M., Hu, Y.: Purification, characterization and immunomodulatory activity of polysaccharides from stem lettuce. Carbohydr. Polym. 188, 236–242 (2018). https://doi.org/10.1016/j.carbpol.2018.02.009

    Article  CAS  PubMed  Google Scholar 

  31. Wang, Y., Zhang, Y., Shao, J., Ren, X., Jia, J., Li, B.: Study on the immunomodulatory activity of a novel polysaccharide from the lichen Umbilicaria Esculenta. Int. J. Biol. Macromol. 121, 846–851 (2019). https://doi.org/10.1016/j.ijbiomac.2018.10.080

    Article  CAS  PubMed  Google Scholar 

  32. Hu, H.B., Liang, H.P., Li, H.M., Yuan, R.N., Sun, J., Zhang, L.L., Han, M.H., Wu, Y.: Isolation, purification, characterization and antioxidant activity of polysaccharides from the stem barks of Acanthopanax leucorrhizus. Carbohydr. Polym. 196, 359–367 (2018). https://doi.org/10.1016/j.carbpol.2018.05.028

    Article  CAS  PubMed  Google Scholar 

  33. Liang, X.X., Gao, Y.Y., Pan, Y., Zou, Y.F., He, M., He, C.L., Li, L.X., Yin, Z.Q., Lv, C.: Purification, chemical characterization and antioxidant activities of polysaccharides isolated from Mycena dendrobii. Carbohydr. Polym. 203, 45–51 (2019). https://doi.org/10.1016/j.carbpol.2018.09.046

    Article  CAS  PubMed  Google Scholar 

  34. Ma, J.S., Liu, H., Han, C.R., Zeng, S.J., Xu, X.J., Lu, D.J., He, H.J.: Extraction, characterization and antioxidant activity of polysaccharide from Pouteria campechiana seed. Carbohydr. Polym. 229, 115409 (2020). https://doi.org/10.1016/j.carbpol.2019.115409

    Article  CAS  PubMed  Google Scholar 

  35. Liang, X., Gao, Y., Fei, W., Zou, Y., He, M., Yin, L., Yuan, Z., Yin, Z., Zhang, W.: Chemical characterization and antioxidant activities of polysaccharides isolated from the stems of Parthenocissus tricuspidata. Int. J. Biol. Macromol. 119, 70–78 (2018). https://doi.org/10.1016/j.ijbiomac.2018.07.131

    Article  CAS  PubMed  Google Scholar 

  36. Liu, Y., Zhao, Y., Yang, Y., Tang, Q., Zhou, S., Wu, D., Zhang, J.: Structural characteristics and hypoglycemic activity of polysaccharides from Coprinus comatus. Bioact. Carbohydr. Diet. Fibre. 2(2), 164–169 (2013). https://doi.org/10.1016/j.bcdf.2013.10.001

    Article  CAS  Google Scholar 

  37. Miao, M., Ma, Y., Jiang, B., Huang, C., Li, X., Cui, S.W., Zhang, T.: Structural investigation of a neutral extracellular glucan from Lactobacillus reuteri SK24.003. Carbohydr. Polym. 106, 384–392 (2014). https://doi.org/10.1016/j.carbpol.2014.01.047

  38. Hui, H., Jin, H., Li, X., Yang, X., Cui, H., Xin, A., Zhao, R., Qin, B.: Purification, characterization and antioxidant activities of a polysaccharide from the roots of Lilium davidii var. unicolor Cotton. Int. J. Biol. Macromol. 135, 1208–1216 (2019). https://doi.org/10.1016/j.ijbiomac.2019.06.030

  39. Charoenwongpaiboon, T., Wangpaiboon, K., Pichyangkura, R., Nepogodiev, S.A., Wonganan, P., Mahalapbutr, P., Field, R.A.: Characterization of a nanoparticulate exopolysaccharide from Leuconostoc holzapfelii KM01 and its potential application in drug encapsulation. Int. J. Biol. Macromol. 187, 690–698 (2021). https://doi.org/10.1016/j.ijbiomac.2021.07.174

    Article  CAS  PubMed  Google Scholar 

  40. Thambiraj, S.R., Phillips, M., Koyyalamudi, S.R., Reddy, N.: Yellow lupin (Lupinus luteus L.) polysaccharides: Antioxidant, immunomodulatory and prebiotic activities and their structural characterisation. Food. Chem. 267, 319–328 (2018). https://doi.org/10.1016/j.foodchem.2018.02.111

  41. Wang, K., Guo, J., Cheng, J., Zhao, X., Ma, B., Yang, X., Shao, H.: Ultrasound-assisted extraction of polysaccharide from spent Lentinus edodes substrate: Process optimization, precipitation, structural characterization and antioxidant activity. Int. J. Biol. Macromol. 191, 1038–1045 (2021). https://doi.org/10.1016/j.ijbiomac.2021.09.174

    Article  CAS  PubMed  Google Scholar 

  42. Zhang, M., Wu, W., Ren, Y., Li, X., Tang, Y., Min, T., Lai, F., Wu, H.: Structural Characterization of a Novel Polysaccharide from Lepidium meyenii (Maca) and Analysis of Its Regulatory Function in Macrophage Polarization in vitro. J. Agric. Food. Chem. 65(6), 1146–1157 (2017). https://doi.org/10.1021/acs.jafc.6b05218

    Article  CAS  PubMed  Google Scholar 

  43. Pautz, A., Art, J., Hahn, S., Nowag, S., Voss, C., Kleinert, H.: Regulation of the expression of inducible nitric oxide synthase. Nitric. Oxide. 23(2), 75–93 (2010). https://doi.org/10.1016/j.niox.2010.04.007

    Article  CAS  PubMed  Google Scholar 

  44. He, Y., Liu, C., Chen, Y., Ji, A., Shen, Z., Xi, T., Yao, Q.: Isolation and structural characterization of a novel polysaccharide prepared from Arca subcrenata Lischke. J. Biosci. Bioeng. 104(2), 111–116 (2007). https://doi.org/10.1263/jbb.104.111

    Article  CAS  PubMed  Google Scholar 

  45. Yuan, Q., Zhao, L., Cha, Q., Sun, Y., Ye, H., Zeng, X.: Structural Characterization and Immunostimulatory Activity of a Homogeneous Polysaccharide from Sinonovacula constricta. J, Agric. Food. Chem. 63(36), 7986–7994 (2015). https://doi.org/10.1021/acs.jafc.5b03306

    Article  CAS  Google Scholar 

Download references

Funding

This work was supported by the Project of Applied Basic Research in Wuhan (Grant No. 2020020601012297) and the Fund for China Agriculture Research System of Edible Fungi (Grant No. CARS-20).

Author information

Author notes

  1. Menghua Shi, Hanyu Zheng & Ruifang Ren

    Present address: National R&D Center for Se-Rich Agricultural Products Processing, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China

Authors and Affiliations

  1. School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430023, China

    Zuo Zhang, Menghua Shi, Hanyu Zheng & Ruifang Ren

  2. National R&D Center for Se-Rich Agricultural Products Processing, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China

    Shaopeng Zhang

  3. Institute of Vegetable, Wuhan Academy of Agricultural Sciences, Wuhan, 430065, China

    Xiaolong Ma

Authors
  1. Zuo Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Menghua Shi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hanyu Zheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ruifang Ren
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Shaopeng Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Xiaolong Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Shaopeng Zhang or Xiaolong Ma.

Ethics declarations

Conflict of interests

There are no conflicts of interest to declare.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Zuo Zhang and Menghua Shi equally contributed to the work.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhang, Z., Shi, M., Zheng, H. et al. Structural characterization and biological activities of a new polysaccharide isolated from Morchella Sextelata. Glycoconj J 39, 369–380 (2022). https://doi.org/10.1007/s10719-022-10058-8

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10719-022-10058-8

Keywords

Search

Navigation