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Enhancement of Edwardsiella piscicida infection, biofilm formation, and motility caused by N-acetylneuraminate lyase

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Abstract

Sialic acid and its catabolism are involved in bacterial pathogenicity. N-acetylneuraminate lyase (NAL), which catalyzes the reversible aldol cleavage of sialic acid to form N-acetyl-D-mannosamine in the first step of sialic acid degradation, has been recently investigated to elucidate whether NAL enhances bacterial virulence; however, the role of NAL in bacterial pathogenicity remains unclear. In the present study, we demonstrated that the existence of two enzymes in Edwardsiella piscicida, referred to as dihydrodipicolinate synthase (DHDPS) and NAL, induced the cleavage/condensation activity toward sialic acids such as N-acetylneuraminic acid, N-glycolylneuraminic acid and 3-deoxy-D-glycero-D-galacto-non-2-ulopyranosonic acid. NAL enhanced cellular infection in vitro and suppressed the survival rate in zebrafish larvae in bath-infection in vivo, whereas DHDPS did not. Furthermore, NAL strongly activated the expression of E. piscicida phenotypes such as biofilm formation and motility, whereas DHDPS did not. Besides, the gene expression level of nanK, nanE, and glmU were up-regulated in the NAL-overexpressing strain, along with an increase in the total amount of N-acetylglucosamine.

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Abbreviations

DHDPS:

Dihydrodipicolinate synthase

GlcNAc:

N-Acetylglucosamine

KDN:

3-Deoxy-D-glycero-D-galacto-non-2-ulopyranosonic acid

Man:

Mannose

ManNAc:

N-Acetylmannosamine

NAL:

N-Acetylneuraminate lyase

Neu5Ac:

N-Acetylneuraminic acid

Neu5Gc:

N-Glycolylneuraminic acid

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Acknowledgements

We appreciate the technical assistance and useful discussions of Dr. Taiki Futagami, Yuko Futagami, Dr. Kazuki Oishi, and Dr. Akinobu Honda. The financial support by JSPS KAKENHI Grant Number 19K06223 is gratefully acknowledged.

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Authors and Affiliations

  1. The United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima, Japan

    Linh Khanh Vo, Masaharu Komatsu & Kazuhiro Shiozaki

  2. Department of Food Life Sciences, Faculty of Fisheries, Kagoshima University, Kagoshima, Japan

    Nhung Thi Tran, Yurina Kubo, Daichi Sahashi, Masaharu Komatsu & Kazuhiro Shiozaki

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  1. Linh Khanh Vo
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Correspondence to Kazuhiro Shiozaki.

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Vo, L.K., Tran, N.T., Kubo, Y. et al. Enhancement of Edwardsiella piscicida infection, biofilm formation, and motility caused by N-acetylneuraminate lyase. Glycoconj J 39, 429–442 (2022). https://doi.org/10.1007/s10719-022-10045-z

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