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Vimentin is important in the neural differentiation of PC12 cells promoted by sialylation

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Abstract

Sialic acid modification is a kind of post-translational modification. To investigate the regulation effect of sialic acid on neural differentiation, we used CycloManN propanyl perac (CycloManN pro), a metabolic precursor of sialic acid, to treat PC12 cells. We noted that CycloManN pro indeed robustly promoted global sialylation detected by MAL II lectin blot in PC12 cells. Simultaneously, we interestingly found that the neurite outgrowth of PC12 cells was significantly promoted by the CycloManN pro treatment. The profile analysis of sialylated proteins showed that a protein band at 55KD was greatly enhanced especially in PC12L cells after CycloManN pro treatment. After enrichment with lectin MAL II, the proteins in this band were analyzed by mass spectrometry. The results showed that 23 proteins were in the band, but the score of vimentin was the highest among them. To investigate further the role of vimentin in the process of neurite differentiation, vimentin construct was transfected into PC12 cells. We interestingly observed that ectopic expression of vimentin significantly enhanced the neurite outgrowth induced by CycloManN pro. However, after three potential glycosylation sites (Ser-7, Thr-33, Ser-34:) of vimentin were mutated to alanine, overexpression of the mutated vimentin completely lost the enhancement activity for the neural differentiation even in the presence of CycloManN pro. Taken together, our study demonstrated that vimentin was important in the induction of neural differentiation by CycloManN pro.

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Acknowledgments

This work was supported by grants from the Natural Science Foundation of China (81271505, 31570800 81571359 and 31400689), the Joint Key Scientific Research Program, Zhejiang Province & Ministry of Public Health, P.R. China (WKJ2012-2-018) and Hangzhou Medical College Academic Incubation Program (00004D11401).

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Authors and Affiliations

  1. Department of Children’s Health Care, The Second Affiliated Hospital & Yuying Children’s Hospital, Wenzhou Medical University, 109 West Xueyuan road, Wenzhou, 325003, China

    Li Hui Wu, Xiao Ting Shao, Jun Xia Guo, Hui Sun, Qi Chen & Jing Pan

  2. Department of Clinical Medicine, Hangzhou Medical College, Hangzhou, China

    Li Hui Wu

  3. Department of Biochemistry and Molecular Biology, Key Lab of Glycoconjugate Research, Ministry of Public Health, School of Basic Medical Sciences, Fudan University, 138 Yi Xue Yuan road, Shanghai, 200032, China

    Hui Sun, Qi Chen, Jing Pan, Qian Qian Cai, Yi Wei Dong, Zhong Yi Chen, Xiao Mei Yan & Xing Zhong Wu

  4. Department of Neonatology, Shanghai first maternity and Child care Hospital, School of Medicine, Tongji University, Shanghai, 200126, China

    Min Peng

  5. New York State Institute for Basic Research in Developmental Disabilities, New York, USA

    Nanbert Zhong

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  1. Li Hui Wu
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Correspondence to Li Hui Wu or Xing Zhong Wu.

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Wu, L.H., Shao, X.T., Guo, J.X. et al. Vimentin is important in the neural differentiation of PC12 cells promoted by sialylation. Glycoconj J 34, 51–59 (2017). https://doi.org/10.1007/s10719-016-9727-6

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