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An α-glucan isolated from root of Isatis Indigotica, its structure and adjuvant activity

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Abstract

The root of Isatis indigotica is a traditional Chinese herbal medicine. An α-glucan (IIP-A-1) was firstly isolated from the roots. In this study we elucidated the chemical structure of IIP-A-1 and determined its adjuvant activity by co-immunizing mice with H1N1 influenza virus split and recombinant hepatitis B surface antigen (HBsAg), respectively. The polysaccharide was pretreated with periodate oxidation, Smith degradation and methylation in order to analyze its structure using GC, HPGPC, FT-IR, NMR and GC-MS. The adjuvant effect was evaluated by determining the antibody titers of serum against H1N1 influenza and HBsAg using ELISA. The proliferation and TNF-α secretion of macrophages administrated with different dose of IIP-A-1 were measured in vitro. The results of this study revealed that IIP-A-1 was an α-glucan with the molecular weight of 3,600 Da. The backbone was α-(1 → 4)-D-glucan with (1 → 6) branch chain. The α-glucan could significantly enhance the immune response of mice immunized with H1N1 influenza or HBsAg in vivo and exert good dose-dependent effects on the proliferation and the TNF-α secretion of macrophages in vitro. These results supported that IIP-A-1 was expected to be an efficacious adjuvant candidate for prophylactic and therapeutic vaccines.

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Abbreviations

AAALAC:

Accreditation of Laboratory Animal Care International

CMC:

1-cyclohexyl–3-(2-morpholinoethyl) carbodiimide metho-p-toluenesulfonate

EI-MS:

electron-impact mass spectrometry

ELISA:

enzyme linked immunosorbent assays

FBS:

fetal bovine serum

FMDV:

foot-and-mouth disease virus

GC:

gas chromatography

GLC:

gas–liquid chromatography and electron-impact mass spectrometry

HBsAg:

hepatitis B virus surface antigen

HPGPC:

high performance gel permeation chromatography

NF-κB:

activating nuclear factor κB

NK:

natural killer

OD:

optical density

PRRS:

porcine reproductive and respiratory syndrome

TFA:

trifluoroacetic acid

TMB:

tetramethyl benzidine

TNF-α:

tumor necrosis factor α.

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Acknowledgments

This work was supported by the funds of New Drug Creation Manufacturing Program (No. 2012ZX09301001-003 and 2012ZX09301003-001).

Conflict of interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. Beijing Institute of Pharmacology and Toxicology, Beijing, 100850, China

    Junjie Shan, Ting Zhu, Peiyuan Jia, Wensheng Qu, Qian Li, Junhua Wu, Hao Ma, Song Wen & Yuxia Wang

  2. Department of Gastroenterology, Chinese PLA Postgraduate Medical School, Beijing, 100853, China

    Guohui Sun

  3. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100190, China

    Jinwei Ren

Authors
  1. Junjie Shan
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  2. Guohui Sun
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  3. Jinwei Ren
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  4. Ting Zhu
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  5. Peiyuan Jia
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  6. Wensheng Qu
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  7. Qian Li
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  8. Junhua Wu
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  9. Hao Ma
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  10. Song Wen
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  11. Yuxia Wang
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Corresponding author

Correspondence to Yuxia Wang.

Additional information

Junjie Shan and Guohui Sun contributed equally to this work.

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Shan, J., Sun, G., Ren, J. et al. An α-glucan isolated from root of Isatis Indigotica, its structure and adjuvant activity. Glycoconj J 31, 317–326 (2014). https://doi.org/10.1007/s10719-014-9525-y

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  • DOI: https://doi.org/10.1007/s10719-014-9525-y

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