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Self-recognition of high-mannose type glycans mediating adhesion of embryonal fibroblasts

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Abstract

High-mannose type N-linked glycan with 6 mannosyl residues, termed "M6Gn2", displayed clear binding to the same M6Gn2, conjugated with ceramide mimetic (cer-m) and incorporated in liposome, or coated on polystyrene plates. However, the conjugate of M6Gn2-cer-m did not interact with complex-type N-linked glycan with various structures having multiple GlcNAc termini, conjugated with cer-m. The following observations indicate that hamster embryonic fibroblast NIL-2 K cells display homotypic autoadhesion, mediated through the self-recognition capability of high-mannose type glycans expressed on these cells: (i) NIL-2 K cells display clear binding to lectins capable of binding to high-mannose type glycans (e.g., ConA), but not to other lectins capable of binding to other carbohydrates (e.g. GS-II). (ii) NIL-2 K cells adhere strongly to plates coated with M6Gn2-cer-m, but not to plates coated with complex-type N-linked glycans having multiple GlcNAc termini, conjugated with cer-m; (iii) degree of NIL-2 K cell adhesion to plates coated with M6Gn2-cer-m showed a clear dose-dependence on the amount of M6Gn2-cer-m; and (iv) the degree of NIL-2 K adhesion to plates coated with M6Gn2-cer-m was inhibited in a dose-dependent manner by α1,4-L-mannonolactone, the specific inhibitor in high-mannose type glycans addition. These data indicate that adhesion of NIL-2 K is mediated by self-aggregation of high mannose type glycan. Further studies are to be addressed on auto-adhesion of other types of cells based on self interaction of high mannose type glycans.

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Abbreviations

CB:

cellobiose (Glcβ1-4Glc)

cer-m:

ceramide mimetic (tetradecylhexadecane)

CCI:

carbohydrate-to-carbohydrate interaction

C/M/W:

a mixture of chloroform methanol and water in a defined volume ratio as indicated case by case

ConA:

Concanavalin A

Glc:

glucose

GlcNAc:

N-acetylglucosamine

GS-II:

Griffonia simplicifolia-II lectin

HPLC:

high performance liquid chromatography

Lex :

Galβ1-4[Fucα1-3]GlcNAcβ1-3Gal

M/W:

methanol and water mixture

NH2cer-m:

aminoceramide mimetic

ODS column:

Shim-pack HRC-octadecyl silica column

Os:

oligosaccharide

PC:

1,2-dimyristoyl-sn-3-phosphocholine

TLC:

thin-layer chromatography

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Acknowledgments

This study was supported by NIH/ NIGMS grant GM070593, and NIH/ NCI R01 CA42505 (to S.H.), and by Grants in Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (to H.Y. and K.K.). The authors like to thank Steve Anderson and Wai Cheu Lai for help in preparation of the manuscript and figures.

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  1. Seon-Joo Yoon

    Present address: Department of New Drug Discovery, Neopharm Co., Ltd., Daejeon, 305-510, Republic of Korea

Authors and Affiliations

  1. Division of Biomembrane Research, Pacific Northwest Research Institute, and Department of Global Health, University of Washington, Seattle, WA, 98122, USA

    Seon-Joo Yoon, Natalia Utkina & Sen-itiroh Hakomori

  2. N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky pr. 47, Moscow, 119991, Russia

    Natalia Utkina

  3. Depart of Chemistry, University of Washington, Seattle, WA, 98195, USA

    Martin Sadilek

  4. Graduate School of Pharmaceutical Sciences, Nagoya City University, Tanabe-dori 3-1, Mizuho-ku, Nagoya, 467-8603, Japan

    Hirokazu Yagi & Koichi Kato

  5. Okazaki Institute for Integrative Bioscience National Institutes of Natural Sciences, 5-1 Higashiyama, Myodaiji, Okazaki, 444-8787, Japan

    Koichi Kato

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Yoon, SJ., Utkina, N., Sadilek, M. et al. Self-recognition of high-mannose type glycans mediating adhesion of embryonal fibroblasts. Glycoconj J 30, 485–496 (2013). https://doi.org/10.1007/s10719-012-9449-3

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