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Bacterial species-characteristic profiles of molecular species, and the antigenicity of phospholipids and glycolipids in symbiotic Lactobacillus, Staphylococcus and Streptococcus species

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Abstract

Human symbiotic bacteria, Lactobacillus reuteri (LR) in the intestines, Staphylococcus epidermidis (SE) in skin and Streptococcus salivalis (SS) in the oral cavity, contain dihexaosyl diglycerides (DH-DG) in concentrations equivalent to those of phosphatidyl glycerol (PG) and cardiolipin (CL), together with mono- to tetrahexaosyl DGs. The molecular species, as the combination of fatty acids in the DG moiety, were revealed to be bacterial species-characteristic, but to be similar between glycolipids and phospholipids in individual bacteria, the major ones being 16:0 and cy19:0 for LR, ai15:0 and ai17:0 for SE, and 16:0 and 18:1 for SS, respectively. The carbohydrate structures of DH-DGs were also bacterial species-characteristic, being Galα1-2Glcα for LR, Glcβ1-6Glcβ for SE, and Glcα1-2Glcα for SS, respectively. Also, bacterial glycolipids were revealed to provide antigenic determinants characteristic of bacterial species on immunization of rabbits with the respective bacteria. Anti-L. johnsonii antiserum intensely reacted with tri- and tetrahexaosyl DGs, in which Galα was bound to DH-DG through an α1-6 linkage, as well as with DH-DG from LR. Although anti-SE antiserum preferentially reacted with DH-DG from SE, anti-SS antiserum reacted with DH-DG from SS and, to a lesser extent, with DH-DGs from LR and SE. But, both anti-SE and anti-SS antiserum did not react at all with monohexaosyl DG or glycosphingolipids with the same carbohydrates at the nonreducing terminals. In addition, 75 % of human sera, irrespective of the ABO blood group, were found to contain IgM to tri- and tetrahexaosyl DGs from LR, but not to DH-DGs from LR, SE and SS.

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Abbreviations

CL:

cardiolipin

DG:

diglycerides

MH-DG:

monohexaosyl diglycerides

DH-DG:

dihexaosyl diglycerides

PA:

phosphatidic acid

PG:

phosphatidyl glycerol

TH-DG:

trihexaosyl diglycerides

TetH-DG:

tetrahexaosyl diglycerides

cy:

cyclopropane

ai:

anteiso

LJ:

Lactobacillus johnsonii

LR:

Lactobacillus reuteri

SE:

Staphylococcus epidermidis

SS:

Streptococcus salivalis

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science and Technology, Kinki University, 3-4-1 Kowakae, Higashiosaka, Osaka, 577-8502, Japan

    Masao Iwamori, Masanori Nakasa, Kentaro Yamazaki & Yuriko Iwamori

  2. Department of Obstetrics and Gynecology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan

    Kyoko Tanaka & Daisuke Aoki

  3. Animal Models of Human Diseases, National Institute of Biomedical Innovation, 7-6-8 Asagi-Saito, Ibaraki, Osaka, 567-0085, Japan

    Shigeki Adachi & Taisei Nomura

Authors
  1. Masao Iwamori
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  2. Masanori Nakasa
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  3. Kentaro Yamazaki
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  4. Yuriko Iwamori
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  5. Kyoko Tanaka
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  6. Daisuke Aoki
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  7. Shigeki Adachi
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  8. Taisei Nomura
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Correspondence to Masao Iwamori.

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Iwamori, M., Nakasa, M., Yamazaki, K. et al. Bacterial species-characteristic profiles of molecular species, and the antigenicity of phospholipids and glycolipids in symbiotic Lactobacillus, Staphylococcus and Streptococcus species. Glycoconj J 29, 199–209 (2012). https://doi.org/10.1007/s10719-012-9393-2

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  • DOI: https://doi.org/10.1007/s10719-012-9393-2

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