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Contribution of glycolipids to species-specific antigens on erythrocytes of several animal species as to recognition of antigens with rabbit anti-glycolipids and anti-erythrocyte antisera

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Abstract

Because anti-glycolipid antibodies are involved in the onset of several neurological diseases, the reactivity of glycolipids on erythrocytes and the probability of generating the antibodies were determined to clarify the contribution of glycolipids as antigens. Anti-erythrocyte antisera reacted with the following glycolipids in a species-specific manner, i.e. blood group A-active glycolipid for man, Forssman glycolipid for sheep, Gg3Cer for guinea pig, and Gg4Cer and fucosyl GM1 for rat, and the hemolytic activities of the anti-erythrocyte antisera were attenuated by absorption of the antisera with liposomes prepared from the lipids of erythrocytes to the following levels, 94.5% for man, 24.5% for sheep, 17.5% for guinea pig, and 54.5% for rat. These species-specific glycolipids on erythrocytes reacted well with the respective anti-glycolipid antisera, but Gb4Cer in man and GM1 in rat were shown to be cryptic on immunization with erythrocytes, indicating that the contribution of glycolipids as erythrocyte antigens differs among animal species.

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Abbreviations

SRBC:

Sheep red blood cells

GRBC:

Guinea pig red blood cells

RRBC:

Rat red blood cells

HRBC:

Human red blood cells

CF:

Complement fixation

ELISA:

Enzyme-linked immunosorbent assay

GVB:

Gelatin-veronal buffer

FGM1:

Fucosyl GM1

SDS-PAGE:

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science and Technology, Kinki University, 3-4-1 Kowakae, Higashiosaka, Osaka, 577-8502, Japan

    Masao Iwamori, Makoto Murata, Mariko Toyoda & Yuriko Iwamori

Authors
  1. Masao Iwamori
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  2. Makoto Murata
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  3. Mariko Toyoda
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  4. Yuriko Iwamori
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Corresponding author

Correspondence to Masao Iwamori.

Additional information

The glycolipid nomenclature is based on the recommendations of the IUPAC-IUB Commission on Biochemical Nomenclature [1]. The ganglioside nomenclature of Svennerholm is employed throughout [2].

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Iwamori, M., Murata, M., Toyoda, M. et al. Contribution of glycolipids to species-specific antigens on erythrocytes of several animal species as to recognition of antigens with rabbit anti-glycolipids and anti-erythrocyte antisera. Glycoconj J 26, 467–476 (2009). https://doi.org/10.1007/s10719-008-9197-6

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  • DOI: https://doi.org/10.1007/s10719-008-9197-6

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