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E-cadherin core fucosylation regulates nuclear β-catenin accumulation in lung cancer cells

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Abstract

E-cadherin expressed highly in 95C and lowly in 95D lung cancer cells which were from the same patient, but core-fucosylated E-cadherin highly expressed in 95D cells. Therefore, Fut8 and Fut8-RNAi constructs were transfected into 95C and 95D cells, respectively. In Fut8-transfectants, reduction of nuclear β-catenin was noted when E-cadherin was core-fucosylated, while accumulation of nuclear β-catenin was observed in Fut8-RNAi transfectants. In E-cadherin-negative MDA-MB-231 cells either Fut8 or Fut8-RNAi transfection couldn't affect nuclear β-catenin. However, cotransfection of E-cadherin with Fut8 caused nuclear β-catenin reduction. Furthermore, enhanced binding of E-cadheirn with β-catenin as well as α-catenin were observed in Fut8-transfectants, and reduction of tyrosine 654 phosphorylation on β-catenin and its transcriptional activity were also noted at the same time. Overall, the current results suggested that core-fucosylated E-cadherin regulated nuclear β-catenin accumulation in lung cancer cells.

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Abbreviations

LCA:

lens culinaris agglutinin

Fut8:

α1,6-fucosyltransferase

siFut8:

Fut RNAi

IP:

Immunoprecipitation

TCF:

T-cell factor

95D cells:

giant cell carcinoma of lung with highly metastatic potential

95C cells:

giant cell carcinoma of lung with low metastatic potential

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Acknowledgement

This work was supported by Natural Science Foundation of China (30070183, 30570414), Shanghai Leading Academic Discipline Project (B110), and Wenzhou Scientific and Technological Bureau (H2006023).

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, 138 Yi Xue Yuan Road, Shanghai, 200032, China

    Ping Hu, Bizhi Shi, Fei Geng, Chunyi Zhang & Xing Zhong Wu

  2. Wenzhou Medical College, Zhejiang, 325035, China

    Wei Wu

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  1. Ping Hu
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  2. Bizhi Shi
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  3. Fei Geng
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  4. Chunyi Zhang
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  5. Wei Wu
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  6. Xing Zhong Wu
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Correspondence to Xing Zhong Wu.

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Hu, P., Shi, B., Geng, F. et al. E-cadherin core fucosylation regulates nuclear β-catenin accumulation in lung cancer cells. Glycoconj J 25, 843–850 (2008). https://doi.org/10.1007/s10719-008-9144-6

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  • DOI: https://doi.org/10.1007/s10719-008-9144-6

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