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Analyses of Fusarium wilt race 3 resistance in Upland cotton (Gossypium hirsutum L.)

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Abstract

Fusarium wilt [Fusarium oxysporum f.sp. vasinfectum (FOV) Atk. Sny & Hans] represents a serious threat to cotton (Gossypium spp.) production. For the last few decades, the FOV pathogen has become a significant problem in Uzbekistan causing severe wilt disease and yield losses of G. hirsutum L. cultivars. We present the first genetic analyses of FOV race 3 resistance on Uzbek Cotton Germplasm with a series of field and greenhouse artificial inoculation-evaluations and inheritance studies. The field experiments were conducted in two different sites: the experimental station in Zangiota region—Environment (Env) 1 and the Institute of Cotton Breeding (Env-2, Tashkent province). The Env-1 was known to be free of FOV while the Env-2 was known to be a heavily FOV infested soil. In both (Env-1 and Env-2) of these sites, field soil was inoculated with FOV race 3. F2 and an F3 Upland populations (“Mebane B1” × “11970”) were observed with a large phenotypic variance for plant survival and FOV disease severity within populations and among control or check Upland accessions. Wilt symptoms among studied F2 individuals and F3 families significantly differed depending on test type and evaluation site. Distribution of Mendelian rations of susceptible (S) and resistant (R) phenotypes were 1S:1R field Env-1 and 3S:1R field Env-2 in the F2 population, and 1S:3R greenhouse site in the F3 population. The different segregation distribution of the Uzbek populations may be explained by differences in FOV inoculum level and environmental conditions during assays. However, genetic analysis indicated a recessive single gene action under high inoculum levels or disease pressure for FOV race 3 resistance. Uzbek germplasm may be more susceptible than expected to FOV race 3, and sources of resistance to FOV may be limited under the FOV inoculum levels present in highly-infested fields making the breeding process more complex.

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Acknowledgments

The authors thank the Office of International Research Programs, US Department of Agriculture (USDA) for funding this study under research grant UZB2-31016-TA-09 and U.S. Civilian Research & Development Foundation (CRDF) for project coordination. We thank also the Academy of Sciences of Uzbekistan and Committee for Coordination of Science and Technology Development of Uzbekistan, Cabinet of Ministers of the Republic of Uzbekistan for continual in-house support of the reserach projects. The USDA is an equal opportunity employer.

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Authors and Affiliations

  1. Center of Genomics and Bioinformatics, Academy of Sciences of Uzbekistan, Ministry of Agriculture & Water Resources of Uzbekistan, and ‘Uzpakhtasanoat’ Association, University street-2, Qibray Region, Tashkent District, 111215, Uzbekistan

    Alisher A. Abdullaev, Ilkhom B. Salakhutdinov, Sharof Sh. Egamberdiev, Azoda T. Adilova & Ibrokhim Y. Abdurakhmonov

  2. Institute of Genetics and Plant Experimental Biology, Academy of Sciences of Uzbekistan, Yuqori Yuz, Qibray Region, Tashkent District, 111226, Uzbekistan

    Alisher A. Abdullaev, Zarif Kuryazov, Ludmila A. Glukhova & Sofiya M. Rizaeva

  3. USDA, ARS, PA, Cropping Systems Research Laboratory, Plant Stress and Germplasm Development Research, Lubbock, TX, 79415, USA

    Mauricio Ulloa

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  1. Alisher A. Abdullaev
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  2. Ilkhom B. Salakhutdinov
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  3. Sharof Sh. Egamberdiev
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  4. Zarif Kuryazov
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  5. Ludmila A. Glukhova
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  6. Azoda T. Adilova
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  8. Mauricio Ulloa
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  9. Ibrokhim Y. Abdurakhmonov
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Correspondence to Ibrokhim Y. Abdurakhmonov.

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Abdullaev, A.A., Salakhutdinov, I.B., Egamberdiev, S.S. et al. Analyses of Fusarium wilt race 3 resistance in Upland cotton (Gossypium hirsutum L.). Genetica 143, 385–392 (2015). https://doi.org/10.1007/s10709-015-9837-2

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