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Identification and distribution of Puroindoline b-2 variant gene homologs in Hordeum

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Abstract

The barley hordoindoline genes (Hina and Hinb) are homologous to the wheat puroindoline genes (Pina and Pinb). These genes are involved in grain hardness, which is an important quality for barley processing. We identified novel variants of Hina and Hinb in 10 wild Hordeum species (H. bogdanii, H. brachyantherum, H. bulbosum, H. chilense, H. comosum, H. marinum, H. murinum, H. patagonicum, H. pusillum, and H. roshevitzii) covering all Hordeum genomes and preliminarily named them Hinc. These nucleotide sequences were highly similar to those of Puroindoline b-2 variant genes (Pinb-2v) and were located on chromosome 7I in H. chilense. The Hinc genes in H. bogdanii, H. bulbosum, H. patagonicum, and H. roshevitzii were pseudogenes possessing in-frame stop codons. We also found a partial Hinc sequence in H. murinum. This gene was not found in cultivated barley and H. vulgare subsp. spontaneum. The phylogenetic tree of Gsp-1, Hin, and Pin genes demonstrates that Hinc and Pinb-2v genes formed one cluster. Therefore, we considered that Hinc and Pinb-2v genes shared a common ancestral gene and were homologous to each other. We also studied the evolutional process of Gsp-1, Hin, and Pin genes. Our results suggested that Gsp-1 might be the most closely related to a putative ancestral gene on Ha locus.

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Acknowledgments

This work was supported by the Bio-oriented Technology Research Advancement Institution. We express our sincere gratitude to Dr. Shin Taketa for providing material support. We also grateful to Dr. Yoichi Hayakawa and Ms. Kimiko Suginohara for their helpful technical assistance.

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Authors and Affiliations

  1. Western Region Agricultural Research Center, NARO, 6-12-1 Nishifukatsu, Fukuyama, 721-8514, Japan

    Yohei Terasawa, Kanenori Takata & Tatsuya M. Ikeda

  2. Laboratory of Plant Genetics and Breeding, Faculty of Agriculture, Saga University, Honjyo 1, Saga, 840-8502, Japan

    Yohei Terasawa & Toyoaki Anai

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  1. Yohei Terasawa
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  2. Kanenori Takata
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  3. Toyoaki Anai
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  4. Tatsuya M. Ikeda
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Correspondence to Tatsuya M. Ikeda.

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Terasawa, Y., Takata, K., Anai, T. et al. Identification and distribution of Puroindoline b-2 variant gene homologs in Hordeum . Genetica 141, 359–368 (2013). https://doi.org/10.1007/s10709-013-9735-4

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  • DOI: https://doi.org/10.1007/s10709-013-9735-4

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