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Origin and molecular organization of supernumerary chromosomes of Prochilodus lineatus (Characiformes, Prochilodontidae) obtained by DNA probes

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Abstract

In Prochilodus lineatus B-chromosomes are visualized as reduced size extra elements identified as microchromosomes and are variable in morphology and number. We describe the specific total probe (B-chromosome probe) in P. lineatus obtained by chromosome microdissection and a whole genomic probe (genomic probe) from an individual without B-chromosome. The specific B-chromosome was scraped and processed to obtain DNA with amplification by DOP-PCR, and so did the genomic probe DNA. Fluorescence in situ hybridization using the B-chromosome probe labeled with dUTP-Tetramethyl-rhodamine and the genomic probe labeled with digoxigenin-FITC permitted to establish that in this species supernumerary chromosomes with varying number and morphology had different structure of chromatin when compared to that of the regular chromosomes or A complement, since only these extra elements were labeled in the metaphases. The present findings suggest that modifications in the chromatin structure of B-chromosomes to differentiate them from the A chromosomes could occur along their dispersion in the individuals of the population.

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Acknowledgments

This work was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) and Centro de Pesquisa e Gestão de Recursos Pesqueiros Continentais (CEPTA/ICMBio). F.P.F. was granted with a fellowship from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP).

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Authors and Affiliations

  1. Laboratório de Genética de Peixes, Departamento Ciências Biológicas, Faculdade de Ciências, Universidade Estadual Paulista (UNESP), Campus de Bauru, Av. Engenheiro Luiz Edmundo C. Coube, s/n, 17033-360, Bauru, SP, Brazil

    Tatiana Aparecida Voltolin, Jehud Bortolozzi & Fábio Porto-Foresti

  2. Escuela de Ciencia y Tecnología, Universidad Nacional de San Martín, Campus Miguelete, 1650, San Martín, Buenos Aires, Argentina

    Alejandro Laudicina

  3. Centro Nacional de Pesquisa e Conservação de Peixes Continentais, CEPTA/ICMBio, Pirassununga, SP, Brazil

    José Augusto Senhorini

  4. Departamento de Morfologia, Instituto de Biociências, Universidade Estadual Paulista, Botucatu, SP, Brazil

    Cláudio Oliveira & Fausto Foresti

Authors
  1. Tatiana Aparecida Voltolin
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  2. Alejandro Laudicina
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  3. José Augusto Senhorini
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  4. Jehud Bortolozzi
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  5. Cláudio Oliveira
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  6. Fausto Foresti
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  7. Fábio Porto-Foresti
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Correspondence to Fábio Porto-Foresti.

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Voltolin, T.A., Laudicina, A., Senhorini, J.A. et al. Origin and molecular organization of supernumerary chromosomes of Prochilodus lineatus (Characiformes, Prochilodontidae) obtained by DNA probes. Genetica 138, 1133–1139 (2010). https://doi.org/10.1007/s10709-010-9502-8

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  • DOI: https://doi.org/10.1007/s10709-010-9502-8

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