Abstract
A single NOR-bearing chromosome pair was identified by silver nitrate staining in a previous study of the fish Prochilodus argenteus from the S ã o Francisco River (MG, Brazil), with a third metacentric chromosome sporadically bearing active NOR. The present study focused on an analysis of the chromosomal localization of both the major (45S) and the minor (5S) rRNA genes using FISH. The use of the 18S rDNA probe confirmed the previous Ag-NOR sites interstitially located in a large metacentric pair and also identified up to three other sites located in the telomeric regions of distinct chromosomes, characterizing an interindividual variation of these sites. In addition, the 5S rDNA site was revealed adjacent to the major NOR site, identified at the end of the large Ag-NOR bearing metacentric chromosome. In a few metaphases, an additional weak hybridization signal was observed in a third chromosome, possibly indicating the presence of another 5S rDNA cluster. Despite a lower karyotype diversification (2n=54 and FN=108) often observed among species of Prochilodontidae, variations involving both 45S and 5S rRNA genes could play an important role in their chromosome diversification.
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Bertollo, L.A.C., C.S. Takahashi & O. Moreira-Filho, 1978. Cytotaxonomic consideration on Hoplias lacerdae (Pisces, Erythrinidae). Rev. Brasil. Genet. 1(2): 103-120.
Born, G.G. & L.A.C. Bertollo, 2000. An XX/XY sex chromo-some system in a sh species, Hoplias malabaricus, with a polymorphic NOR-bearing X chromosome. Chrom. Res. 8: 111-118.
Castro, R.M.C., 1990. Revisão taxonômica da família Proc-hilodontidae (Ostariophysi, Characiformes). PhD thesis, Universidade de São Paulo, Ribeirão Preto, Brazil.
Castro, J., A. Viñas, L. Sánchez & P. Martínez, 1996. Characterization of an atypical NOR site polymorphism in brown trout (Salmo trutta )with Ag-and CMA3-staining, and fluorescent in situ hybridization. Cytogenet. Cell Genet. 75: 234-239.
Childs, G., R. Maxson, R.H. Cohn & L. Kedes, 1981. Orphons: dispersed genetic elements derived from tandem repetitive genes of eucaryotes. Cell 23(3): 651-663.
Drouin, G. & M. Muniz de Sá, 1995. The concerted evolution of 5S ribosomal genes linked to the repeat units of other multigene families. Mol. Biol. Evol. 12: 481-493.
Feldberg, E., J.I.R. Porto & L.A.C. Bertollo, 1992. Karyotype evolution in Curimatidae (Teleostei, Characiformes)of Amazon region. I. Studies on the genera Curimata, Psec-trogaster, Steindachnerina and Curimatella. Rev. Bras. Genet. 15(2): 369-383.
Ferro, D.A.M., D.M. Néo, O. Moreira-Filho & L.A.C. Bertollo, 2001. Nucleolar organizing regions, 18S and 5S rDNA in Astyanax scabripinnis (Pisces, Characidae): pop-ulations distribution and functional diversity. Genetica 110: 55-62.
Flecker, A.S., 1996. Ecosystem engineering by a dominant detritivore in a diverse tropical stream. Ecology 77: 1845-1854.
Foresti, F., L.F. Almeida-Toledo & S.A. Toledo-Filho, 1981. Polymorphic nature of nucleolus organizer region in fishes. Cytogenet. Cell Genet. 31: 137-144.
Fujiwara, A., S. Abe, E. Yamaha, F. Yamasaki & M.C. Yoshida, 1998. Chromosomal localization and heterochro-matin association of ribosomal RNA genes loci and silver stained nucleolar organizer regions in salmonid fishes. Chrom. Res. 6: 463-471.
Galetti, Jr. P.M., 1998. Chromosome diversity in Neotropical fishes: NOR studies. Ital. J. Zool. 65(Suppl.): 53-56.
Galetti, Jr. P.M., F. Foresti, L.A.C. Bertollo & O. Moreira-Filho, 1981. Karyotipic similarity in three genera (Lepori-nus, Leporellus and Schizodon )of the family Anostomidae (Pisces, Teleostei). Braz. J. Genet. 4: 11-15.
Galetti, Jr. P.M., A.C.G. Cesar & P.C. Venere, 1991. Hetero-chromatin and NORs variability in Leporinus sh (Anos-tomidae, Characiformes). Caryologia 44(3-4): 287-292.
Howell, W.M. & D.A Black, 1980. Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia 36: 1014-1015.
Jesus, C.M. & O. Moreira-Filho, 2000. Karyotypes of three species of Parodon (Teleostei: Parodontidae). Ichthyol. Explor. Freshwaters 11(1): 75-80.
Jesus, C.M. & O. Moreira-Filho, 2003. Chromosomal location of 5S and 18S rRNA genes in Prochilodus lineatus (Char-aciformes, Prochilodontidae). Caryologia 56(3): 281-287.
Long, E.O. & I.D. David, 1980. Repeated genes in eukaryotes. Annu. Rev. Biochem. 49: 727-764.
Lucchini, S., I. Nardi, G. Barsacchi, R. Batistoni & F. Andronico, 1993. Molecular cytogenetics of the ribosomal (18S +28S and 5S)DNA loci in primitive and advanced urodele amphibians. Genome 36: 762-773.
Mago-Leccia, E., 1972. Consideraciones sobre la sistematica de la familia Prochilodontidae (Osteichthyes, Cypriniformes), con una sinopsia de las especies de Venezuela. Acta Biol. Venez. 8(1): 35-96.
Martínez, P., A. Viñas, C. Bouza, J. Arias, R. Amaro & L. Sánchez, 1991. Cytogenetical characterization of hatchery stocks and natural populations of Sea and Brown trout from northwestern Spain. Heredity 66: 9-17.
Martínez, J.L., P. Morán, S. Garcia-Vásquez & A.M. Pendás, 1996. Chromosomal localization of the major and 5S rRNA genes in the European eel (Anguilla anguilla). Cytogenet. Cell Genet. 73: 149-152.
Martins, C. & P.M. Galetti Jr., 1998. Karyotype similarity between two sympatric Schizodon sh species (Anostomi-dae, Characiformes)from the Paraguay River basin. Gen. Mol. Biol. 21(3): 355-360.
Martins, C. & P.M. Galetti Jr., 1999. Chromosomal stability of 5S rDNA clusters in Leporinus sh (Anostomidae, Charac-iformes). Chrom. Res. 7: 363-367.
Martins, C. & P.M. Galetti Jr., 2000. Conservative distribution of 5S rDNA loci in Schizodon (Pisces, Anostomidae) chromosomes. Chrom. Res. 44: 903-910.
Martins, C. & P.M. Galetti Jr., 2001a. Two 5S rDNA arrays in Neotropical sh species: is it a general rule for shes? Genetica 111: 439-446.
Martins, C. & P.M. Galetti Jr., 2001b. Organization of 5S rDNA in species of the sh Leporinus: two di.erent genomic locations are characterized by distinct nontran-scribed spacers. Genome 44: 903-910.
Miller, O.J., D.A. Miller, V.G. Dev, R. Tantravahi & C.M. Croce, 1976. Expression of human and suppression of mouse nucleolus organizer activity in mouse-human so-matic cell hybrids. Proc. Antl. Acad. Sci. USA 73: 4531-4535.
Morán, P., J.L. Martínez, E. Garcia-Vásquez & A.M. Pendás, 1996. Sex linkage of 5S rDNA in rainbow trout (Oncorhyn-chus mykiss). Cytogenet. Cell Genet. 75: 145-150.
Moreira-Filho, O., L.A.C. Bertollo & P.M. Galetti Jr., 1984. Structure and variability of nucleolar organizer regions in Parodontidae fish. Can. J. Gen. Cytol. 26(5): 564-568.
Murakami, M. & H. Fujitani, 1998. Characterization of repetitive DNA sequences carrying 5S rDNA of the triploid ginbuna (Japanese silver crucian carp, Carassius auratus langsdor). Genes Genet. Syst. 73: 9-20.
Paiva, M.R., 1983. Peixes e pescas de águas interiores do Brasil. Editerra Editorial, Brasília, 158p.
Pauls, E. & L.A.C. Bertollo, 1983. Evidence for a system supernumerary chromosomes in Prochilodus scrofa Steind-achner, 1881 (Pisces-Prochilodontidae). Caryologia 36(4): 307-314.
Pauls, E. & L.A.C. Bertollo, 1990. Distribution of supernu-merary chromosome system and aspects of karyotypic evolution in the genus Prochilodus (Pisces, Prochilodonti-dae). Genetica 81: 117-123.
Pendás, A.M., P. Morán & E. García-Vázquez, 1993. Multi-chromosomal localization of ribosomal RNA genes and heterochomatin association in brown trout. Chrom. Res. 1: 63-67.
Pendás, A.M., P. Morán, J.P. Freije & E. García-Vásquez, 1994. Chromosomal mapping and nucleotide sequence of two tandem repeats of Atlantic salmon 5S rDNA. Cytogenet. Cell Genet. 67: 31-36.
Schmid, M., L. Vitelli & R. Batistoni, 1987. Chromosome banding in Amphibia. IV. Constitutive heterochromatin, nucleolus organizers, 18S +28S and 5S ribosomal RNA 243. genes in Ascaphidae, Pipidae, Discoglossidae and Pelobat-idae. Chromosoma 95: 271-284.
Suzuki, H., S. Sakurai & Y. Matsuda, 1996. Rat rDNA spacer sequences and chromosomal assignment of the genes to the extreme terminal region of chromosome 19. Cytogenet. Cell Genet. 72: 1-4.
Venere, P.C. & P.M. Galetti Jr., 1989. Chromosome evolution and phylogenetic relationships of some Neotropical Char-aciformes of the family Curimatidae. Brazil. J. Genet. 12(1): 17-25.
Vicente, V.E., C.M. Jesus & O. Moreira-Filho, 2001. Chromo-somal localization of 5S and 18S rRNA genes in three Parodon species (Pisces, Parodontidae). Caryologia 54(4): 365-369.
Viñas, A., C. Gómez, P. Martínez & L. Sánchez, 1996. Localization of rDNA genes in European eel (Anguilla anguilla )by FISH. Genome 39: 1220-1223.
Vitelli, L., R. Batistoni, F. Andronico, I. Nardi & G. Barsacchi-Pilone, 1982. Chromosomal localization of 18S +28S and 5S ribosomal RNA genes in evolutionary divergent anuran amphibians. Chromosoma 84: 475-491.
Wasko, A.P., C. Martins, J.M. Wright & P.M. Galetti Jr., 2001. Molecular organization of 5S rDNA in shes of the genus Brycon. Genome 44: 893-902.
White, T.J., T. Bruns, S. Lee & J. Taylor, 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. in PCR Protocols: a Guide to Methods and Applications. Academic Press Inc., pp. 315-322.
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Hatanaka, T., Galetti, P.M. Mapping of the 18S and 5S ribosomal RNA genes in the fish Prochilodus argenteus Agassiz, 1829 (Characiformes, Prochilodontidae). Genetica 122, 239–244 (2004). https://doi.org/10.1007/s10709-004-2039-y
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DOI: https://doi.org/10.1007/s10709-004-2039-y